Genomic DNA and total RNA from tumor and adjacent liver parenchyma were extracted using the ZR-Duet DNA and RNA MiniPrep Plus kit (Zymo Research) following the manufacturer's instructions. Prior to extraction, biopsies were crushed in liquid nitrogen to facilitate lysis. Total RNA of 15 patients without HCC was extracted using Trizol (Thermo Fisher Scientific) according to the manufacturer's instructions. Extracted DNA was quantified using the Qubit Fluorometer (Invitrogen). Extracted RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), and RNA quality/integrity was assessed with an Agilent 2100 BioAnalyzer using RNA 6000 Nano Kit (Agilent Technologies).
Zr duet dna and rna miniprep plus kit
Manufactured by Zymo Research
Sourced in Germany
The ZR-Duet DNA and RNA MiniPrep Plus kit is a lab equipment product designed for the simultaneous isolation and purification of both DNA and RNA from a single biological sample. The kit utilizes a quick and efficient spin-column based method to extract and purify nucleic acids from a variety of sample types.
Automatically generated - may contain errors
Lab products found in correlation
Qubit fluorometer, by Thermo Fisher Scientific (4 mentions)
Trizol, by Thermo Fisher Scientific (4 mentions)
Agilent 2100 bioanalyzer, by Agilent Technologies (2 mentions)
Rna 6000 nano kit, by Agilent Technologies (2 mentions)
Dneasy blood tissue kit, by Qiagen (2 mentions)
Nanodrop 2000, by Thermo Fisher Scientific (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (2 mentions)
Dneasy blood and tissue kit, by Qiagen (1 mentions)
5 protocols using zr duet dna and rna miniprep plus kit
1
Extraction and Quantification of Genomic DNA and RNA
2
Extraction of Genomic DNA and RNA from Tissue Biopsies
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Genomic DNA and total RNA from biopsies from the discovery cohort were extracted using the ZR‐Duet DNA and RNA MiniPrep Plus Kit (Zymo Research, Freiburg im Breisgau, Germany) following the manufacturer’s instructions. Prior to extraction, biopsies were crushed in liquid nitrogen to facilitate lysis. Extracted DNA was quantified using the Qubit Fluorometer (Invitrogen, Waltham, MA, USA) [17 (link)]. DNA from peripheral blood leucocytes (‘buffy coat’) was extracted using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. For the validation cohort, DNA was extracted using the ChargeSwitch Genomic DNA Mini Tissue Kit (Invitrogen) following the manufacturer’s instructions [8 (link)].
3
Tumor Organoid DNA Extraction
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Genomic DNA from tumor organoids was extracted using the DNeasy Blood & Tissue kit (Qiagen) following the manufacturer’s instructions. Genomic DNA and total RNA from biopsies were extracted using the ZR-Duet DNA and RNA MiniPrep Plus kit (Zymo Research) following the manufacturer’s instructions. Prior to extraction, biopsies were crushed in liquid nitrogen to facilitate lysis. Extracted DNA was quantified using the Qubit Fluorometer (Invitrogen).
4
Extraction of Genomic DNA and RNA
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Genomic DNA and total RNA from biopsies were extracted using the ZR-Duet DNA and RNA MiniPrep Plus kit (Zymo Research) following the manufacturer’s instructions. Before extraction, biopsies were crushed in liquid nitrogen to facilitate lysis. Genomic DNA from organoids was extracted using the DNeasy Blood & Tissue kit (Qiagen) following the manufacturer’s instructions. Total RNA from organoids was extracted using TRIzol reagent (Thermo Fisher Scientific).
5
Comprehensive Tissue Extraction Protocol
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Genomic DNA and total RNA from tumor and adjacent liver parenchyma were extracted using the ZR-Duet DNA and RNA MiniPrep Plus kit (Zymo Research) following the manufacturer’s instructions. Prior to extraction, biopsies were crushed in liquid nitrogen to facilitate lysis. Total RNA of 15 patients without HCC was extracted using Trizol (Thermo Fisher Scientific) according to the manufacturer’s instructions. Extracted DNA was quantified using the Qubit Fluorometer (Invitrogen). Extracted RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), and RNA quality/integrity was assessed with an Agilent 2100 BioAnalyzer using RNA 6000 Nano Kit (Agilent Technologies).
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