Ldh cytotoxicity assay kit

Anti-LC3B antibody was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The Go6976 and bafilomycin A1 (BAF1) were obtained from Selleck, Inc. (Houston, TX, USA). Anti-phospho-PKC-α antibody was purchased from Millipore (Billerica, MA, USA). Diacylglycerol (DAG) enzyme-linked immunosorbent assay (ELISA) kit was purchased from USCN, Inc. (Wuhan, China). MitoTracker^® Red CMXRos was purchased from Invitrogen, Inc. (Paisley, UK). Bromodeoxyuridine (BRDU) was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Anti-PKC-α antibody was purchased from Bioworld Technology, Inc. (St. Louis Park, MN, USA). Anti-Mfn1, Mfn2, Opa1, Nix, Parkin and ULK1 antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Alexa Fluor 488 was purchased from Jackson ImmunoResearch Inc. (West Grove, PA, USA). Anti-FUNDC1 polyclonal antibody was purchased from Aviva PLC, Inc. (San Diego, CA, USA). Cell counting kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). LDH cytotoxicity assay kit was purchased from Keygen Biotech, Co. (Nanjing, China).

Anti-microtubule-associated protein-light chain 3 (LC3)B (cat. no. 2775) and anti-cleaved caspase-3 (cat. no. 9662) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Alexa Fluor 488 (cat. no. 705-546-147) was purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA). Cell counting Kit-8 (CCK-8; cat. no. WO97/38985) was purchased from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). The LDH Cytotoxicity Assay kit was purchased from Nanjing KeyGen Biotech Co., Ltd (Nanjing, China). A triglyceride (TG) assay kit (GPO-POD; cat. no. E1003) was purchased from (Applygen Technologies Inc., Beijing, China). The in situ cell death detection kit (cat. no. 11684795910) was purchased from Sigma-Aldrich; (Merck KGaA, Darmstadt, Germany). Anti-Atg5 antibody (cat. no. ab227132) was purchased from Abcam (Cambridge, UK). Recombinant PEDF was synthesized by Cusabio Biotech Co., Ltd. (Hubei, China). The special inhibitor of autophagy 3-methyladenine (3-MA; cat. no. KGATGR006; Nanjing KeyGen Biotech Co., Ltd.) was donated from Dr Xiaofang Yang (Laboratory of Clinical and Experimental Pathology, Xuzhou Medical University, Jiangsu, China).