Intravitreal injection was performed at 24 weeks of diabetes. All mice were immunosuppressed through intraperitoneal injection using cyclosporine (30 mg/kg) daily for 7 days before intravitreal injection and then biweekly for the next 3 weeks. The pupils were dilated using 1% atropine sulfate ophthalmic solution (AKORN) and 2.5% phenylephrine hydrochloride (Paragon BioTeck Inc.). Mice were anesthetized using 2.5% isoflurane (Fluriso, VetOne). A cell suspension containing 1 × 105 KNA+ cells in 1 μl of saline was slowly injected into the vitreous cavity through the sclera/choroid using a 32-gauge beveled needle (Hamilton Company, Reno, NV) (64 (link)). Another cohort of mice receiving 1 μl of saline injection into the vitreous cavity was used as control. Topical triple antibiotic ointment (Allegan) was applied to the injected eye.
32 gauge beveled needle
Manufactured by Hamilton Company
The 32-gauge beveled needle is a small, precision medical instrument used for various laboratory and healthcare applications. It features a thin, tapered design with a beveled tip, allowing for accurate and minimally invasive procedures. The needle's dimensions and construction make it suitable for a range of delicate tasks, but its specific intended use should be determined by qualified professionals.
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Lab products found in correlation
2 protocols using 32 gauge beveled needle
1
Intravitreal Injection of KNA+ Cells
2
Intracameral Adeno-Associated Virus Delivery
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Three-month-old C57BL/6J wild-type or FP receptor knockout mice previously generated by our laboratory [39 (link)] were anesthetized with an intraperitoneal injection of ketamine/xylazine/acepromazine (80/6/1 mg/kg body weight) for intracameral injections. Mice were subsequently placed on a dissecting microscope to visualize the anterior chamber. A 32 gauge beveled needle (Hamilton Company, Reno, NV) containing the AAV2 construct (1 μL; 3 x 1012 VG/mL) or 1 μL phosphate buffered saline (PBS) was placed parallel to the cornea and inserted anterior to the limbus into the anterior chamber. Care was taken to avoid iris trauma as well as avoidance of the corneal endothelium and anterior lens capsule. The injected volume was administered into the anterior chamber over 2 seconds. The needle was then slowly removed to minimize reflux.
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