Anti human lc3 4e12

Manufactured by MBL Life Science

Anti-human LC3 (4E12) is a monoclonal antibody that specifically binds to the human microtubule-associated protein 1 light chain 3 (LC3). LC3 is a key protein involved in the process of autophagy, a cellular mechanism for the degradation and recycling of damaged or unwanted cellular components.

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Lab products found in correlation

Rapamycin, by InvivoGen (1 mentions) Carbamazepine, by Bio-Techne (1 mentions) Bafilomycin a1, by InvivoGen (1 mentions) Everolimus, by InvivoGen (1 mentions) Goat anti mouse igg1 af488, by Thermo Fisher Scientific (1 mentions) Dnase type 1, by Worthington (1 mentions) Dispase 2, by Roche (1 mentions) Collagenase d, by Roche (1 mentions) Lightning link rapid antibody labeling kit, by Abcam (1 mentions) Facscanto 2, by BD (1 mentions)

Close spelling variants of this product

Anti-LC3

2 protocols using anti human lc3 4e12

Multiparametric Flow Cytometry Analysis

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The following antibodies were used: anti-human CD1a (HI149-APC; BD Pharmigen), CD3 (UCTH1-APC-FIRE750; Biolegend), anti-human CD8a (HIT8a-PerCP-Cy5.5), anti-human CD14 (HCD14-PE-Dazzle594), anti-human CD11c (B-ly6-PE-Cy7), anti-HIV-1 capsid protein p24 (KC57-PE; Beckman Coulter), anti-human LC3 (4E12; MBL Life Science), goat anti-mouse IgG1 (AF488; Invitrogen A-21121).
The following reagents were used: bafilomycin A1 (Invivogen), carbamazepine (Tocris), everolimus (Invivogen), rapamycin (Invivogen), Zidovudine (AZT; NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH), paraformaldehyde (8%, Electron Microscopy Sciences, Aurion), dispase II (Roche Diagnostics), ethylenediaminetetraacetic acid (EDTA; Sigma-Aldrich), 2 mM 1,4-dithiothreitol (DTT; Sigma-Aldrich), Collagenase D (Roche), DNAse type I (Worthington Biochemical Corporation).

Cloherty A.P., van Teijlingen N.H., Eisden T.J., van Hamme J.L., Rader A.G., Geijtenbeek T.B., Schreurs R.R, & Ribeiro C.M. (2021). Autophagy-enhancing drugs limit mucosal HIV-1 acquisition and suppress viral replication ex vivo. Scientific Reports, 11, 4767.

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Quantifying Autophagy in Dengue-Infected Cells

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Cells were either left untreated or pre-treated with bafilomycin A1 (In vivogen tlrl-baf, 50nM) for 4h followed by infection with DENV-2/16681 (MOI=6) for 48h. Quantification of intracellular LC3 II levels by saponin extraction was performed as described before (26, (link)27, (link)52, (link)58) . In assays using dengue-infected DCs, intracellular staining of LC3-II was performed using a two-step staining with anti-human LC3 (4E12; MBL Life Science) followed by goat anti-mouse IgG1 (AF488; Invitrogen A-21121). In assays using non-infected genotyped DCs, intracellular staining of LC3-II was performed using a single-step staining with anti-LC3 monoclonal antibody (4E12; MBL Life Science) directly conjugated to AF488 using the Lightning-Link ® Rapid Antibody Labeling kit (Expedeon), according to the manufacturer's instructions. Intracellular LC3-II levels were quantified using flow cytometry (FACSCanto II, BD Biosciences).

Cloherty A.P.M., Rader A.G., Patel K.S., Eisden T.T.H.D., van Piggelen S., Schreurs R.R.C.E, & Ribeiro C.M.S. (2024). Dengue virus exploits autophagy vesicles and secretory pathways to promote transmission by human dendritic cells. Frontiers in immunology, 15.

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