Rat PCC PC12 cells were acquired from American Type Culture Collection ATCC (Manassas, VA, USA) and cultured in our laboratory. Dulbecco's Modifi ed Eagle Medium (DMEM) was acquired from Mediatech (Herndon, VA, USA). Fetal bovine serum (FBS) and 6-OHDA (6-Hydroxydopamine) were acquired from Sigma-Aldrich (St Louis, MO, USA). All primary antibodies (antiβ-actin, anti-pAKT, anti-PTEN, anti-AKT) were acquired from Cell Signaling Technology (Boston, MA, USA).
Rat pcc pc12 cells
Manufactured by American Type Culture Collection
Sourced in United States
Rat PCC PC12 cells are a widely used cell line derived from pheochromocytoma cells of the rat adrenal medulla. These cells exhibit many characteristics of sympathetic neurons and are commonly used in neuroscience research to study neuronal differentiation, signaling pathways, and other cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Anti β actin, by Cell Signaling Technology (2 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Anti pten, by Cell Signaling Technology (1 mentions)
6 hydroxydopamine 6 ohda, by Merck Group (1 mentions)
Anti cyclin b1, by Cell Signaling Technology (1 mentions)
Nvp auy922, by Selleck Chemicals (1 mentions)
Anti heat shock protein 70 hsp70, by Cell Signaling Technology (1 mentions)
Anti bax, by Cell Signaling Technology (1 mentions)
Anti phospho mek, by Cell Signaling Technology (1 mentions)
Horse serum, by Merck Group (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Anti mek, by Cell Signaling Technology (1 mentions)
17 aag, by Selleck Chemicals (1 mentions)
2 protocols using rat pcc pc12 cells
1
Rat PCC PC12 Cell Culture and Analysis
2
Rat PC12 Cells: HSP90 Inhibitor Effects
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Rat PCC PC12 cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% horse serum, 5% fetal bovine serum (FBS; Sigma-Aldrich, St Louis, MO, USA), and antibiotic/antimycotic at 37°C with 5% CO2. The HSP90 inhibitor, NVP-AUY922, and 17-AAG were purchased from Selleck Chemicals (Houston, TX, USA) and solubilized in dimethyl sulfoxide (DMSO) to a concentration of 1 mM/mL before use. Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Tokyo, Japan). All primary antibodies (anti-β-actin, anti-cyclin B1, anti-Bax, anti-heat-shock protein 70 (HSP70), anti-phospho-AKT (S473), anti-AKT, anti-phospho-ERK1/2 (T202/Y204), anti-ERK1/2, anti-phospho-MEK, and anti-MEK) were acquired from Cell Signaling Technology (Boston, MA, USA).
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