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Third generation enzyme linked immunosorbent assay

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The third-generation enzyme-linked immunosorbent assay (ELISA) is a laboratory technique used to detect and quantify specific proteins, antibodies, or other molecules in a sample. It utilizes enzymes to amplify a signal, allowing for highly sensitive and accurate measurements. The core function of this ELISA is to provide a quantitative analysis of the target analyte in the sample.

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Lab products found in correlation

Radioimmunoassay, by Abbott (1 mentions) Versant hcv rna quantitative 3.0 assay, by Siemens (1 mentions)

2 protocols using third generation enzyme linked immunosorbent assay

1

Hepatitis C and B Viral Serology

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Hepatitis C virus antibodies and hepatitis B surface antigen were examined at study entry. Hepatitis C virus antibodies were detected with a third-generation enzyme-linked immunosorbent assay (Abbott Laboratories, North Chicago, IL). Hepatitis B surface antigen was detected by radioimmunoassay (Abbott Laboratories).
Kawamura Y., Ikeda K., Arase Y., Sorin Y., Fukushima T., Kunimoto H., Hosaka T., Kobayashi M., Saitoh S., Sezaki H., Akuta N., Suzuki F., Suzuki Y, & Kumada H. (2015). New discriminant score to predict the fibrotic stage of non-alcoholic steatohepatitis in Japan. Hepatology International, 9(2), 269-277.
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2

Serum HCV Antibody and RNA Detection

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Serum HCV antibodies were detected by second-generation enzyme-linked immunosorbent assay and then confirmed by third-generation enzyme-linked immunosorbent assay (Abbott Lab, Chicago, IL, USA) in the first 18 patients enrolled in this study. The last method was consistently employed in the remaining 42 patients. Serum HCV RNA was determined by RT-PCR (Roche Diagnostics, Branchburg, NJ, USA) and quantified with the Versant HCV RNA quantitative 3.0 assay (Siemens Healthcare, Erlangen, Germany). HCV genotyping was performed with INNO-LiPA (Innogenetics NV, Ghent, Belgium).
Sansonno D., Russi S., Sansonno S., Pavone F, & Dammacco F. (2015). Thymic stromal lymphopoietin in hepatitis C virus-related cryoglobulinemic vasculitis: gene expression level and protein distribution. Arthritis Research & Therapy, 17(1), 62.
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