This study was approved by the Ethics Committee of Tianjin Medical University Cancer Institute and Hospital. All guardians have signed the informed consent. The clinical samples used in this study were all obtained from department of pediatric oncology. SK-N-SH, SK-N-BE(2), Hela, Du145, and HEK293T cell lines were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). SK-N-SH, SK-N-BE(2), Hela and four transfected cell lines, sh-control/SK-N-SH, sh-PLK4/SK-N-SH, sh-control/SK-N-BE(2), and sh-PLK4/SK-N-BE(2), were all cultured in minimum essential medium (MEM; Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, HyClone, USA). The Du145 and HEK293T cell lines was cultured in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% FBS (HyClone). All cells were supplemented with 1% penicillin–streptomycin solution (PS, HyClone) and cultured in a 5% CO2 and humidified incubator maintained at 37 °C.
Sk n sh
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
SK-N-SH is a cell line derived from a human neuroblastoma. It is used for research purposes in neurobiology and cancer biology.
Automatically generated - may contain errors
Lab products found in correlation
Imr 32, by National Collection of Authenticated Cell Cultures (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Hek293t, by Thermo Fisher Scientific (2 mentions)
Sk n sh, by Thermo Fisher Scientific (2 mentions)
Sh sy5y, by National Collection of Authenticated Cell Cultures (2 mentions)
Sk n as, by American Type Culture Collection (2 mentions)
Hek293t, by National Collection of Authenticated Cell Cultures (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Du145, by National Collection of Authenticated Cell Cultures (1 mentions)
Du145, by Thermo Fisher Scientific (1 mentions)
Sk n be 2, by Thermo Fisher Scientific (1 mentions)
Sk n be, by Thermo Fisher Scientific (1 mentions)
Sk n be 2, by American Type Culture Collection (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Sk n as, by Thermo Fisher Scientific (1 mentions)
Sk n be 2 c, by American Type Culture Collection (1 mentions)
Sk n be 2 c, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
6 protocols using sk n sh
1
Ethical Cell Culture Practices for Cancer Research
2
Neuroblastoma Clinical and Cell Line Study
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Patients diagnosed with neuroblastoma in Xin Hua Hospital, affiliated with Shanghai Jiao Tong University School of Medicine, in the period of Sep. 2016 to July 2019, were enrolled to collect clinical information and tumor samples for further investigation. All patients were treated according to the Chinese Children Cancer Group-NB-2014 (CCCG-NB-2014) protocol [13 ]. This study was approved by the Ethics Committee of Xin Hua Hospital affiliated with Shanghai Jiao Tong University School of Medicine. The cell lines SK-N-SH, IMR-32, SH-SY5Y, and 293T were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). SK-N-BE(2) and SK-N-AS were purchased from ATCC (Manassas, USA). SK-N-SH, IMR-32, SK-N-BE(2), and 293T were all cultured in DMEM supplemented with 10% fetal bovine serum(FBS) and 1% penicillin-streptomycin solution. SH-SY5Y and SK-N-AS cell lines were cultured in a 1:1 mixture of MEM and F12 Medium with 1% Gluta-max, 1% Sodium pyruvate, 1% NEAA, 1% penicillin-streptomycin solution, and 10% FBS. The mediums and FBS were all purchased from Gibco, USA. All cells grew in a humidified incubator with 5% CO2 at 37°C.
3
Culturing Neuroblastoma Cell Lines
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The SK-N-BE(2)C, CHLA20, HEK293T cell lines were obtained from the American Type Culture Collection (ATCC) (Manassas, VA), and the SK-N-AS, SK-N-SH, SK-N-BE(2), KP-N-NS, and IMR-32 cell lines were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). All of the cell lines were tested routinely for mycoplasma contamination by PCR assay (Vazyme). SK-N-BE(2)C and SK-N-SH cell lines were cultured in F12/DMEM 1:1 (Gibco, Life Technologies) containing 10% fetal bovine serum (FBS) (Gibco, Life Technologies), 1% penicillin and streptomycin (Gibco, Life Technologies), and HEK293T and SK-N-AS were cultured in DMEM containing 10% FBS and 1% penicillin and streptomycin. All of the cell lines were cultured at 37°C in a humidified incubator with 5% CO2.
4
Culturing NB Cell Lines
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Six NB cell lines (SK-N-BE, GNP, SH-SY5Y, IMR-32, LAN-1, and SK-N-SH) were provided by the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The cells were cultured in RPMI-1640 medium (HyClone Laboratories Inc., Logan, Utah, USA) containing 10% fetal bovine serum (FBS, Life Technologies, cat. no. 10270) and 1% penicillin/streptomycin (Gibco, Gaithersburg, MD, USA) at 37°C in an incubator with 5% CO2.
5
Transfecting Human Neuroblastoma Cell Lines
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Human neuroblastoma cell lines (SK-N-BE [2 (link)], SK-N-AS, SH-SY5Y) were obtained from ATCC (Manassas, USA), SK-N-SH, IMR-32 were from Chinese Academy of Sciences Cell Bank (Shanghai, China). All of the cells were routinely maintained in a 1:1 mixture of Eagle’s Minimum Essential Medium and Ham’s nutrient mixture F-12 Medium supplemented with 10% fetal bovine serum (Gibco, USA) at 37 °C with 5% CO2. The human siRNA MCM6 and the non-targeting siRNA (sequences was in Table S1 ) were purchased from Riobio Biotechnology (Guangzhou, China). The cells were transfected with siRNA at a final concentration of 60 nM for 24 h performing in Lipofectamine™ RNAimax (Thermo Fisher Scientific, USA). To be specific, cultured the cells in a 6-well plate and performed transfection when the cell density was about 30%. In 200 ul Opti MEM I medium, mixed 0.12 nmol siRNA with 8 μl transfection reagent and incubated for 5 min, added to one well of a 6-well plate, and then add 2 ml complete medium. After 24 h of incubation, the medium was replaced with a standard medium and the cells were ready for further experiments.
6
Cultivation of Human Neuroblastoma Cell Lines
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The human neuroblastoma cell lines SH-SY5Y, SK-N-BE2, and SK-N-SH were obtained from the Chinese Academy of Sciences Cell Bank (Shanghai, China). Cell cultivation followed the procedures outlined in a prior study [28 ].
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