Vitrification solution

Manufactured by Kitazato

Sourced in Japan

Kitazato vitrification solution is a cryoprotective medium used for the vitrification of cells or tissue samples. It is designed to facilitate the rapid cooling and warming of samples to preserve their structural and functional integrity during cryopreservation.

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Lab products found in correlation

Cryotop, by Kitazato (1 mentions) Thawing solution, by Kitazato (1 mentions)

5 protocols using vitrification solution

Efficient Cryopreservation of Embryos

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Embryos were cryopreserved on day 3, 5, or 6 of embryo culture. The embryos were
placed into equilibrium solution (Kitazato Corporation, Tokyo, Japan) for 6
minutes in room temperature, transferred to vitrification solution (Kitazato
Corporation) for 30 s, and then loaded on a Cryotop (Kitazato Corporation) and
plunged into liquid nitrogen within 60 s, for no longer than 90 s after initial
exposure to vitrification solution. For thawing, the Cryotop was removed from
liquid nitrogen and placed immediately into thawing solution (Kitazato
Corporation) at 37°C for 1 minute, followed by a three-step rehydration
protocol: dilution solution for 3 minutes, followed by two steps of washing
solution for 5 minutes, respectively. The embryos were then transferred into a
droplet of blastocyst medium in a pre-balanced culture dish in 37°C and 6.0%
CO₂.

Qi Q., Luo J., Wang Y, & Xie Q. (2020). Effects of artificial cycles with and without gonadotropin-releasing hormone agonist pretreatment on frozen embryo transfer outcomes. The Journal of International Medical Research, 48(6), 0300060520918474.

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Vitrification of Human Embryos for PGD

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Three days after ICSI, the embryos moved into microdrops of biopsy media (LG PGD BIOPSY medium, Life Global) that were under mineral oil. Zona pellucida drilling was performed mechanically and one blastomere was biopsied for PGD analysis. The embryo was transferred into a drop for embryo vitrification. The embryo was maintained into an equilibration solution (Kitazato BioPharma Co., Ltd., Japan) for 15 min at room temperature (20-27°C), after an initial shrinkage and recovery. Subsequently, the embryo was aspirated and placed in the vitrification solution (Kitazato BioPharma Co., Ltd., Japan) at room temperature for a period of 1 min and then placed on a cryotop with a minimum volume of VS solution (24). Cryotop quickly plunged them into liquid nitrogen.

Dorfeshan P., Ghaffari Novin M., Salehi M, & Farifteh F. (2019). Expression of miR-302 in human embryo derived from in-vitro matured oocyte. International Journal of Reproductive Biomedicine, 17(6), 405-412.

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Blastocyst Vitrification and Transfer

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Blastocysts were vitrified after biopsy using Kitazato vitrification solution (Kitazato Biopharma Co., Ltd., Shizuoka, Japan) and closed high-security vitrification straws (Cryo Bio System, L’Aigle, France). After warming and dilution, blastocysts were cultured in blastocyst medium for 1–2 h. The chromosomally normal blastocysts and surviving re-expanded blastocysts with high morphological grades were selected for preferential transfer. No more than two blastocysts were transferred, and a single blastocyst transfer to each patient with well-cryopreserved embryos was recommended.

Cheng D., Hu L., Gong F., Yuan S., Luo K., Wu X., Xie P., Lu C., Lu G., Tan Y.Q, & Lin G. (2021). Clinical outcomes following preimplantation genetic testing and microdissecting junction region in couples with balanced chromosome rearrangement. Journal of Assisted Reproduction and Genetics, 38(3), 735-742.

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Vitrification and Transfer of Chromosomally Normal Blastocysts

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Blastocysts were vitrified after the biopsy using Kitazato vitrification solution (Kitazato Biopharma Co. Ltd. Shizuoka. Japan) and closed High Security Vitrification straws (Cryo Bio System, France). Each blastocyst was stored in an individual straw. After warming and dilution, blastocysts were cultured in blastocyst medium for 1–2 h. Only chromosomally normal/balanced blastocysts were selected for warming, and the surviving re-expanded blastocysts with high morphological grades were selected for transfer. Luteal support was applied in cryopreserved embryo transfer (CET) cycles. Warmed blastocysts were transferred either 5 days after ovulation during a natural menstrual cycle or 5 days after the initiation of ovulation via progesterone administration. Briefly, 6 mg Estradiol Valerate was started from Day 3 for 10–15 days, then luteal support was applied when a satisfactory endometrial development (thickness ≥ 8 mm) was confirmed with ultrasound. No more than two blastocysts were transferred, and single blastocyst transfer to each patient with well-cryopreserved embryos was recommended.

Hu L., Cheng D., Gong F., Lu C., Tan Y., Luo K., Wu X., He W., Xie P., Feng T., Yang K., Lu G, & Lin G. (2016). Reciprocal Translocation Carrier Diagnosis in Preimplantation Human Embryos. EBioMedicine, 14, 139-147.

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Vitrification and Warming of Biopsied Blastocysts

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Blastocysts were vitrified after the biopsy using Kitazato vitrification solution (Kitazato Biopharma Co. Ltd. Shizuoka. Japan) and closed High Security Vitrification straws (Cryo Bio System, France). The vitrification and warming procedure was carried out according to the protocol recommended in the Kitazato vitrification kit. Each blastocyst was stored in an individual straw. After warming and dilution, blastocysts were cultured in blastocyst medium for 1–2 hours. We selected the chromosomally normal/balanced blastocysts for warming, and the surviving re-expanded blastocysts with high morphology grade were selected for transfer.

Tan Y., Yin X., Zhang S., Jiang H., Tan K., Li J., Xiong B., Gong F., Zhang C., Pan X., Chen F., Chen S., Gong C., Lu C., Luo K., Gu Y., Zhang X., Wang W., Xu X., Vajta G., Bolund L., Yang H., Lu G., Du Y, & Lin G. (2014). Clinical outcome of preimplantation genetic diagnosis and screening using next generation sequencing. GigaScience, 3, 30.

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