T47D (ATCC® HTB-133™), MCF7 (ATCC® HTB-22™) and MDA-MB-231 (ATCC® HTB-26™) breast cancer cell lines were obtained from the American Type Culture Collection (Manassas, VA). All cancer cell lines were grown in DMEM/F12 culture medium (Sigma, St. Louis, MO, USA) supplemented with 10% FBS (Sigma), penicillin (Sigma) (100 IU/mL), and streptomycin (Sigma) (100 μg/mL) at 37°C in a humidified atmosphere with 5% CO2.
T47d atcc htb 133
Manufactured by American Type Culture Collection
Sourced in United States
T47D (ATCC® HTB-133™) is a cell line derived from a human breast ductal carcinoma. It is commonly used in research related to breast cancer.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Penicillin, by Merck Group (2 mentions)
Streptomycin, by Merck Group (2 mentions)
Dmem f12 culture medium, by Merck Group (1 mentions)
Mcf 7 atcc htb 22, by American Type Culture Collection (1 mentions)
Mda mb 231 atcc htb 26, by American Type Culture Collection (1 mentions)
Mda mb 468, by Merck Group (1 mentions)
Dmem nutrient f 12 ham dmem f12 culture medium, by Merck Group (1 mentions)
Mcf 7, by Merck Group (1 mentions)
Mda mb 231, by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
Bt 549 atcc htb 122, by American Type Culture Collection (1 mentions)
2 protocols using t47d atcc htb 133
1
Breast Cancer Cell Line Culturing
2
Culturing Human Breast Cancer Cell Lines
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T47D (ATCC® HTB-133™), MCF7 (ATCC® HTB-22™), MDA-MB-231 (ATCC® CRM-HTB-26™), MDA-MB-468 (ATCC® HTB-132™) and BT-549 (ATCC® HTB-122™) human BC cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). T47D, MCF7, MDA-MB-231 and MDA-MB-468 cancer cells were grown in DMEM/Nutrient F-12 Ham (DMEM/F12) culture medium (Sigma) with 10% FBS (Sigma), penicillin (100 IU/mL) (Sigma) and streptomycin (100 μg/mL) (Sigma). BT-549 cells were maintained in Roswell Park Memorial Institute Medium (RPMI) culture medium (ATTC) with 0.023 U/mL insulin (Sigma), 10% FBS (ATTC), penicillin (100 IU/mL) (Sigma) and streptomycin (100 μg/mL) (Sigma). Mycoplasma-free cultures were maintained in a humidified atmosphere with 5% CO2 at 37°C.
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