Briefly, adult rats or mice were anesthetized by intraperitoneal injection of tribromoethanol (300 mg/kg; Sigma). After complete anesthesia, each rat or mouse was connected to a rodent ventilator (MouseVent, Kent Scientific Corp., Torrington, CT, USA). Under fully-controlled ventilation, a thoracotomy was made in the left intercostal space between the third and fourth ribs, and the heart was exposed by removal of the pericardium. The LAD coronary artery was permanently ligated using a 6-0 nylon suture. The thoracic cavity and skin were immediately closed with 4-0 suture, and the operation field was smeared with Iodophor and injected with 10,000 units of penicillin solution to prevent infection. Body temperature was maintained at 37°C on a heating pad until complete recovery. Sham-operated animals were subjected to the same procedures except that the suture was passed under the LAD but not tied.
Mousevent
Manufactured by Kent Scientific
Sourced in United States
MouseVent is a ventilator designed for small animal research. It provides precise control over respiratory parameters to facilitate physiological studies. The device features adjustable tidal volume, respiratory rate, and inspiratory/expiratory ratios to accommodate a range of experimental needs.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using mousevent
1
Surgical Induction of Myocardial Infarction in Rodents
2
Rat Model of Myocardial Infarction
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The rat MI model was created by ligation of the LAD coronary artery in male wild-type rats at the age of 8–10 weeks as previously described (Du et al., 2022 (link)). Briefly, each rat was anesthetized by intraperitoneal injection of tribromoethanol (300 mg/kg; Sigma-Aldrich, St Louis, MO, USA). After complete anesthesia, each rat was immobilized in the supine position, intubated and connected to a small-animal ventilator on an operating table (MouseVent, Kent Scientific Corp., Torrington, CT, USA). Under controlled ventilation, the thoracotomy was made in the left intercostal space between the third and fourth ribs, and the heart was exposed by removing the pericardium. The LAD coronary artery was permanently ligated using a 6-0 non-absorbable surgical suture, and the chest and skin were immediately sutured. The rat was finally removed from the ventilator and kept warm until revived. Rats with sham surgery were subjected to the same procedure without performing the ligation.
3
Myocardial Ischemia-Reperfusion Injury Model
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Three hours before surgery, adult male Sprague–Dawley rats (8 weeks old) were administered DSF (50 mg/kg) by oral gavage. The rats were then anesthetized by intraperitoneal injection of tribromoethanol (300 mg/kg; Sigma-Aldrich) and ventilated on a rodent ventilator (MouseVent, Kent Scientific Corp., Torrington, CT, USA). A thoracotomy between the third and fourth ribs was performed to expose the heart. Myocardial IR was induced by tightening a slipknot around the left anterior descending coronary artery using a 6–0 nylon suture for 30 min/45 min and then loosening it (reperfusion for 24 h/28 d) before collecting the heart.
4
Myocardial Infarction Model in Rodents
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The MI model was created by ligation of the LAD coronary artery in male rats or mice at the matched age of 8–10 weeks as previously described and without reperfusion22 (link). Briefly, each animal was anesthetized by intraperitoneal injection of pentobarbital sodium (30 mg/kg). After complete anesthesia, animals were immobilized in the supine position on an operating table, intubated, and connected to a small-animal ventilator (MouseVent, Kent Scientific Corp., Torrington, CT, USA). Under fully-controlled ventilation, a thoracotomy was made in the left intercostal space between the third and fourth ribs, and the heart exposed by removal of the pericardium. The LAD coronary artery was permanently ligated using a 6–0 (for rats) or 7–0 (for mice) non-absorbable surgical suture, and the chest and skin immediately closed. Animals were finally removed from the ventilator and kept warm until completely revived. Animals with sham surgery were subjected to the same procedure without performing the LAD ligation step.
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