Jc 1 stain kit

McCoy's 5A medium, fetal bovine serum (FBS), penicillin-streptomycin and trypsin-EDTA were obtained from Gibco (Grand Island, NY, USA). NAC, Ac-DEVD-CHO, DAPI and AO&EB, DCFH-DA, JC-1 stain kit, Cell and Tissue mitochondria isolation kits, Caspase-8 activity assay kit and BCA protein assay kit were obtained from Beyotime Biotech (Jiangsu, China). Nuclear-Cytosol kit was obtained from Applygen Technologies Inc (Beijing, China). Annexin V-FITC/PI assay kit, ROS and TNF-α ELISA kits were purchased from KeyGEN Biotech (Nanjing, China). Antibodies against Bax (^#2772), Bcl-2 (^#4223), Cytochrome c (^#11940), Caspase-3 (^#9662), cleaved Caspase-3 (^#9661), Caspase-8 (^#4790), cleaved Caspase-8 (^#9496), Caspase-9 (^#9508), NF-κB (p65, ^#8242) and Histone H3 (^#3638) were purchased from Cell Signalling Technology (Danvers, MA, USA). Anti-β-actin and anti-GAPDH antibodies, anti-mouse and anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibodies, 5-Fluorouracil (5-FU), MTT and standard sugar were purchased from Sigma (St. Louis, MO, USA). All other chemicals used were of analytical grade.

Human Burkitt lymphoma cell lines Raji and Ramos were donated by Jiangsu Key Laboratory of the Noncoding RNA Foundation and Clinical Transformation of Yangzhou University. The RPMI 1640 cell culture medium (GIBCO) and fetal bovine serum (FBS) (Hyclone Laboratories Inc.) were purchased from Abbkine Scientific (China). CCK8 was also obtained from Abbkine Scientific (China). A hematoxylin-eosin staining kit was purchased from Guangdong Baso (China). A cell cycle kit was purchased from KeyGEN BioTECH (China). The EdU staining kit, JC-1 stain kit, and Annexin V-PI kit were purchased from Shanghai Beyotime (China). Monoclonal antibodies against Bcl-2, BAX, Cleaved-caspase-3, and Bcl-xL were purchased from Cell Signaling Technology (United States). The β-actin antibody was obtained from Sigma-Aldrich (United States).