Bay 11 7082

The reagents 4-MU (CID: 5280567), ketotifen fumarate (CID: 5282408), Evans blue (CID: 9409), toluidine blue (CID: 7083), and NF-κB inhibitor BAY 11-7082 (CID: 5353431) were purchased from Meilun Biotechnology Co., Ltd. (Dalian, China). ERK inhibitor U0126 (CID: 3006531), JNK inhibitor SP600125 (CID: 8515), and p38 inhibitor SB203580 (CID: 176155) were purchased from MedChem Express (Monmouth Junction, NJ, USA). DNP-HSA, monoclonal DNP-specific IgE and OVA were obtained from Sigma-Aldrich (St. Louis, MO, USA). Aluminum adjuvant was purchased from Thermo Scientific (Waltham, MA, USA). Rabbit monoclonal IgG primary antibodies against JNK (c-Jun N-terminal kinase), phosphorylated (p)-JNK (Thr183/Tyr185), p-SYK (spleen tyrosine kinase, Tyr525/ Tyr526), ERK (extracellular signal-regulated kinase), p-ERK (Thr202/Tyr204), p38, p-p38 (Thr180/Tyr182), and anti-GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (Cell Signaling Technology, Danvers, MA). We bought rabbit monoclonal primary antibodies targeting SYK, p65, and IκBα from Abcam (Cambridge, MA) and bought IgGκ BP-horse radish peroxidase (HRP), anti-rabbit IgG-HRP, and monoclonal IgG anti-p-p65 (Ser311) primary antibodies from Santa Cruz Biotechnology (Dallas, TX, USA).

Dulbecco’s Modified Eagle Medium (DMEM) and Roswell Park Memorial Institute (RPMI) 1640 were purchased from KeyGEN BioTECH (Nanjing, China). Fetal bovine serum (FBS) was obtained from GIBCO (New York, NY, USA). Juglone (a inhibitor of Pin1), DMSO, and oil red O were purchased from Sigma-Aldrich (St. Louis, MO, USA). BAY11-7082 and oxLDL were obtained from Meilunbio (Dalian, China). Calcein-AM was purchased from YEASEN (Shanghai, China). The primary antibodies against VCAM-1 and F4/80 were obtained from Abcam (Cambridge, UK). Rabbit antibodies against MCP-1, ERK1/2, p-ERK1/2, JNK, p-JNK, p65, and p-p65 were purchased from Cell Signaling Technology (Beverly, MA, USA). Rabbit anti-Pin1 antibody was obtained from Proteintech (Chicago, IL, USA). HRP-conjugated antibody against β-actin was purchased from Kangchen Biotechnology (Shanghai, China).