To prepare bacterial cellulose, the Gluconacetobacter hansenii GH-1/2008 strain from the collection of VKPM B-10547 (Gause Institute of New Antibiotics, Moscow, Russia) was used. The strain is non-toxic and non-pathogenic to humans [40 ]. The used medium was composed of (g/L): sucrose (20.0), peptone (5.0), yeast extract (5.0), Na2HPO4 (2.7) and citric acid monohydrate (1.15). The inoculum was prepared by growing G. hansenii on this medium with the use of a rotary shaker ThermoStable IS-20 DAIHAN Scientific Co., Seoul, Korea at 30 °C for 3 days. Upon completion of the cultivation, the prepared bacterial cellulose films were separated from the culture broth via filtration, repeatedly washed with distilled water to remove the medium components, treated with 1.0 M NaOH solution at 80 °C for 2 h to remove cells and other impurities immobilized on the films, and finally washed with distilled water until a neutral pH of wash liquid was reached. The detailed preparation of the BC films was described elsewhere [41 (link)].
The degree of BC polymerization was determined by the intrinsic viscosity measurement of its solutions in cadoxen according to ASTM D1795-96 and ASTM D4243-99 standards [42 (link)]. The average value of polymerization degree was 900.
The degree of BC polymerization was determined by the intrinsic viscosity measurement of its solutions in cadoxen according to ASTM D1795-96 and ASTM D4243-99 standards [42 (link)]. The average value of polymerization degree was 900.