125i udr

Manufactured by PerkinElmer

Sourced in Denmark

125I-UdR is a radioactive tracer compound used for research purposes. It consists of the nucleoside uridine labeled with the radioactive isotope iodine-125. This compound can be utilized in various scientific applications that require the monitoring or detection of cellular processes involving DNA synthesis.

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Lab products found in correlation

De52 cellulose, by Cytiva (1 mentions) Iodogen, by Merck Group (1 mentions)

2 protocols using 125i udr

Radiolabeled Tracers for Glioma Studies

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¹²⁵I-UdR was either purchased from Perkin Elmer (Skovlunde, Denmark) or prepared essentially as described by Wang et al. 17 (link) using trimethylstannyl-2'-deoxyuridine as precursor. The final product was sterilized by passing the eluate through a 0.22 µm sterile filter into a capped vial. MTX (Sigma-Aldrich, Copenhagen, Denmark) and TMZ (Sigma-Aldrich) was prepared according to the manufacturer's instructions. MTX (1 mM) and TMZ (129 mM) were stored at -20 °C and diluted in fresh medium for in vitro studies right before each use.
The amino-acid tracer [¹¹C]methylaminoisobutyric acid ([¹¹C]MeAIB) was prepared as described previously 18 .

Thisgaard H., Halle B., Aaberg-Jessen C., Olsen B.B., Therkelsen A.S., Dam J.H., Langkjær N., Munthe S., Någren K., Høilund-Carlsen P.F, & Kristensen B.W. (2016). Highly Effective Auger-Electron Therapy in an Orthotopic Glioblastoma Xenograft Model using Convection-Enhanced Delivery. Theranostics, 6(12), 2278-2291.

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Radiolabeling and Targeting of Monoclonal Antibodies

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To target the cytoplasm, ¹²⁵I was coupled to the internalizing mouse m225 mAb. ¹²⁵I-labeled m225 mAb is internalized within the cytoplasm after binding to its receptor (HER1). For cell surface localization, ¹²⁵I was coupled to the noninternalizing mouse IgG1k mAb 35A7. For the CEA Gold 2 epitope (50 (link)), 35A7 is specific. The nonspecific PX antibody was used as a control (irrelevant antibody). PX is an IgG1 mAb purified from mouse myeloma P3-X63 cells (30 (link)). The three mAbs were obtained from mouse hybridoma ascites fluids by ammonium sulfate precipitation followed by ion exchange chromatography on DE52 cellulose (Whatman, Balston, United Kingdom).
Antibody radiolabeling was performed according to the IODO-GEN (1,3,4,6-tetrachloro-3α, 6α-diphenylglycoluryl) (Sigma-Aldrich, St. Louis, MO) method (50 (link)). ¹²⁵I-mAb-specific activity was 370 MBq/mg of protein, and immunoreactivity was 60%–95%. All experiments used ¹²⁵I-mAb activities ≤4 MBq/ml. ¹²⁵I was targeted to the nucleus using the thymidine base analogue ¹²⁵I-UdR at a specific activity of 81.4 TBq/mmol. ¹²⁵I-UdR was purchased from PerkinElmer (Waltham, MA).

Paillas S., Ladjohounlou R., Lozza C., Pichard A., Boudousq V., Jarlier M., Sevestre S., Le Blay M., Deshayes E., Sosabowski J., Chardès T., Navarro-Teulon I., Mairs R.J, & Pouget J.P. (2016). Localized Irradiation of Cell Membrane by Auger Electrons Is Cytotoxic Through Oxidative Stress-Mediated Nontargeted Effects. Antioxidants & Redox Signaling, 25(8), 467-484.

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