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Sigenome smartpool sirna targeting

Manufactured by Horizon Discovery
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SiGENOME SMARTpool siRNA targeting is a lab equipment product designed for gene silencing experiments. It consists of a pool of pre-designed and validated small interfering RNAs (siRNAs) targeting a specific gene of interest. The core function of this product is to facilitate efficient and specific knockdown of target gene expression in various cell lines and experimental models.

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Lab products found in correlation

Dharmafect 1 reagent, by Horizon Discovery (2 mentions) Sigenome non targeting sirna pool 1, by Horizon Discovery (2 mentions) On targetplus smartpool sirna targeting, by Horizon Discovery (1 mentions) On targetplus non targeting pool, by Horizon Discovery (1 mentions)

Close spelling variants of this product

SMARTpool (292 citations) SMARTpool siRNA (284 citations) SiGENOME SMARTpool (203 citations) SiRNAs (161 citations) SiGENOME SMARTpool siRNA (134 citations) SiGENOME siRNA (20 citations) SiRNA targeting (10 citations)

3 protocols using sigenome smartpool sirna targeting

1

FYN Knockdown Assay Protocol

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SiGENOME SMARTpool siRNA targeting FYN and siGENOME Non-Targeting siRNA Pool #1 (Dharmacon) were used at 50 nM. Transfection was performed using Dharmafect 1 reagent. Transfection efficiency was confirmed to be ≥90% using siGENOME positive control targeting GAPDH (Supporting Information Fig. S5c). RNA extraction and process outgrowth assay were performed 96h after transfection.
Nelson M., Millican-Slater R., Forrest L.C, & Brackenbury W.J. (2014). The sodium channel β1 subunit mediates outgrowth of neurite-like processes on breast cancer cells and promotes tumour growth and metastasis. International Journal of Cancer. Journal International du Cancer, 135(10), 2338-2351.
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2

Investigating FYN gene function via siRNA

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SiGENOME SMARTpool siRNA targeting FYN and siGE-NOME Non-Targeting siRNA Pool #1 (Dharmacon) were used at 50 nM. Transfection was performed using Dharmafect 1 reagent. Transfection efficiency was confirmed to be ≥90% using siGENOME positive control targeting GAPDH (Supporting Information Fig. S5c). RNA extraction and process outgrowth assay were performed 96h after transfection.
Nelson M., Millican-Slater R., Forrest L.C, & Brackenbury W.J. (2014). The sodium channel β1 subunit mediates outgrowth of neurite-like processes on breast cancer cells and promotes tumour growth and metastasis. International Journal of Cancer. Journal International du Cancer, 135(10), 2338-2351.
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3

Investigating PPARγ, UBR5, and ATMIN Depletion

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ON-TARGETplus SMARTpool siRNA targeting PPARγ
(L-003436-00-0005; Dharmacon) was used to deplete PPARγ for all
experiments. For verifying γH2AX suppression, individual siRNA of the
pool were used (#7, J-003436-07-0005; #8, J-003436-08-0005; #9,
J-003436-09-0005, Dharmacon). For depleting UBR5, we used siGENOME SMARTpool
siRNA targeting UBR5 (M-007189-02-0010, Dharmacon). For depleting ATMIN, we
used ON-TARGETplus SMARTpool siRNA targeting ATMIN (L-020304-01-0010,
Dharmacon). ON-TARGETplus non-targeting pool (D-001810-10-05, Dharmacon) was
used for all siControl transfections.
Li C.G., Mahon C., Sweeney N.M., Verschueren E., Kantamani V., Li D., Hennigs J.K., Marciano D.P., Diebold I., Abu-Halawa O., Elliott M., Sa S., Guo F., Wang L., Cao A., Guignabert C., Sollier J., Nickel N.P., Kaschwich M., Cimprich K.A, & Rabinovitch M. (2019). PPARγ Interaction with UBR5/ATMIN Promotes DNA Repairto Maintain Endothelial Homeostasis. Cell reports, 26(5), 1333-1343.e7.
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