Alp assay kit

SAOS-2 cells were seeded on PEEK samples, which were not BSA blocked, at a concentration of 5 × 10⁴ cells/cm², and cultured in growth media at 37 °C in 5% CO₂ until confluence. At confluence, the media in selected samples were augmented with osteogenic supplements for the duration of the assay. The media were changed every 48 h during culture and 24 h before testing for ALP activity.
At 1, 7, 10, and 14 days post-confluence, extracellular ALP activity was measured with an ALP assay kit (Abnova). The resulting absorbance was then quantified by comparison to a standard curve of known ALP activity.

ALP activity was determined by the ALP assay kit (Abnova) according to the manufacturer’s instructions. On the test day, the cells were disrupted and corrected by total protein content with the BCA assay kit (KeyGEN,). The absorbance was read at 405 nm in a microplate reader (MK3, Thermo).