Mib1 clone mib1

Manufactured by Agilent Technologies

Sourced in Italy, Denmark

Mib1 (clone Mib1) is a laboratory equipment product offered by Agilent Technologies. This product serves as a tool for protein analysis and research. The core function of Mib1 is to facilitate the study and understanding of the Mib1 protein, which plays a role in various biological processes. Detailed information about the intended use or specific applications of this product is not available in this unbiased and factual description.

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Lab products found in correlation

Anti cd20 clone l26, by Agilent Technologies (1 mentions) Anti cd68 antibody clone pg m1, by Agilent Technologies (1 mentions) Anti cd138 clone mi15, by Agilent Technologies (1 mentions) Anti mum1 clone mum1p, by Agilent Technologies (1 mentions) Cd34 clone qbend10, by Agilent Technologies (1 mentions) Alpha smooth muscle actin clone 1a4, by Agilent Technologies (1 mentions)

3 protocols using mib1 clone mib1

Comprehensive Bone Marrow Biopsy Analysis in MM

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In all cases, bone marrow biopsy evaluation was performed as a part of the staging workup for MM at iliac spine. In selected patients (cases 16, 17, and 18), typical HU < 0 LBLs underwent to needle biopsy. In detail, 3-μm-thick slides were obtained from formalin-fixed, decalcified paraffin-embedded marrow samples. Following the routine protocol for bone marrow examination, the slices were stained with H&E, PAS, and Giemsa. Marrow fibrosis was assessed by reticulin stain. The immunohistochemical characterization of plasma cells was performed using the following antibodies: anti-CD138 (clone MI15 Dako, Glostrup, Denmark), anti-MUM1 (clone MUM1p, Dako, Glostrup, Denmark), anti-CD56 (clone 504 Leica, Milan, Italy), anti-cyclin D1 (clone EP12, Dako, Glostrup, Denmark), anti-CD20 (clone L26, Dako, Glostrup, Denmark), anti-CD79a (clone 11E3, Leica, Milan, Italy), anti-CD3 (clone LN10, Novocastra, Milan, Italy), and Mib1 (clone Mib1 Dako, Glostrup, Denmark). An anti-CD68 antibody (clone PGM1, Dako, Glostrup, Denmark) was used to assess the presence and distribution of peri-trabecular osteoclasts.

Zambello R., Crimì F., Lico A., Barilà G., Branca A., Guolo A., Varin C., Vezzaro R., Checuz L., Scapin V., Berno T., Pizzi M., Ponzoni A., De Biasi E., Vio S., Semenzato G., Zucchetta P, & Lacognata C. (2018). Whole-body low-dose CT recognizes two distinct patterns of lytic lesions in multiple myeloma patients with different disease metabolism at PET/MRI. Annals of Hematology, 98(3), 679-689.

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Ki-67/MIB-1 Staining Quantification in Prostate Tissue

Cited 1 time

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Prostate tissue staining for Ki-67/MIB-1 has been described previously (25 (link)). The monoclonal antibody, MIB-1 (clone MIB-1, DAKO) was used to determine the proportion of cancer epithelial, cancer-associated stromal and benign-associated stromal cells staining positive for Ki-67. Prostate cancer tissue microarray slides were scanned on Aperio ScanScope AT (Aperio Technologies, Vista, CA, USA). High-resolution 20× digital images were created for the cancer and benign cores of twenty randomly selected cases. Positive Ki-67-stained cells and the total number of cells in 20× fields were counted using ImageJ2 Cell Counter plug-in (ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA). Any nuclear staining, regardless of intensity, was considered positive for Ki-67/MIB-1. For the stromal compartment, only spindle-like cells were included in the analysis, while round small nuclei cells were not considered for immunohistochemical evaluation, thus avoiding the inclusion of inflammatory cell in the analysis. The number of Ki-67 positive cells was expressed as a percentage of immunoreactive stromal (or epithelial) cells to the total counted stromal cells (or epithelial) in a 20× field.

Gomez-Sarosi L., Sun Y., Coleman I., Bianchi-Frias D, & Nelson P.S. (2017). DNA Damage Induces a Secretory Program in the Quiescent TME that Fosters Adverse Cancer Phenotypes. Molecular cancer research : MCR, 15(7), 842-851.

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GIST Differential Diagnosis by IHC

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Immunohistochemical staining was performed to distinguish GIST from other mesenchymal tumors in addition to hematoxylin and eosin staining. For the immunohistochemical staining, commercially available primary antibodies against KIT (polyclonal, 1:500; MBL, Nagoya, Japan), CD34 (clone QBEnd10, 1:600; Dako, Glostrup, Denmark), S100 (polyclonal, 1:1000; Dako), alpha smooth muscle actin (clone 1A4, 1:1000; Dako) and MIB-1(clone MIB-1, 1:1000; Dako) were utilized.

Funasaka K., Miyahara R., Furukawa K., Sawada T., Maeda K., Yamamura T., Ishikawa T., Ohno E., Nakamura M., Kawashima H., Hirooka Y., Ohmiya N, & Fujishiro M. (2020). Mutation analysis of gastrointestinal stromal tumors using RNA obtained via endoscopic ultrasound-guided fine-needle aspiration. Translational Oncology, 13(11), 100848.

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