Anti gpcr gpr49 lgr5

Antibodies used include: anti-CD44 (Cell Signaling Technology, Danvers, MA); anti-DCLK1, anti-CD45, anti-EpCAM, anti-CK19 and anti-GPCR GPR49 (Lgr5) (Abcam, Cambridge, MA); anti-AnxA2 (BD Biosciences, Carlsbad, CA), and anti-β-actin (total) (Sigma, St Louis, MO). Anti-PG antibody was generated in our laboratory as described [27 (link)). Alexa Fluor-594 and Alexa Fluor-488 coupled secondary IgG were from Invitrogen (Carlsbad, CA). Three kits from Stem Cell Technologies were used: 1) RosetteSep™ Human CD45 Depletion Cocktail (#1522), 2) RosetteSep™ Human Circulating Epithelial Tumor Cell Enrichment Cocktail (#15127), and 3) EasySep™ Human Whole Blood CD45 Depletion Kit (#18289), (Vancouver, Canada).

Antibodies used in this study include: anti-DCLK1, anti-PCNA and anti-GPCR GPR49 (Lgr5) (Abcam, Cambridge, MA); anti-E-cadherin (Cell Signaling, Boston, MA); anti-active caspase-3 (Millipore, Temecula, CA) and anti-β-actin (total) (Sigma, St Louis, MO). Alexa Fluor-594 and Alexa Fluor-488 coupled secondary IgG were purchased from Invitrogen (Carlsbad, CA). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) was purchased from Life Technologies (Grand Island, NY). Saline (0.9% Sodium Chloride Injection, USP) was purchased from Hospira (Lake Forest, IL). Thrombin peptide TP508, a 23 amino acid peptide AGYKPDEGKRGDACEGDSGGPFV, also known as rusalatide acetate or Chrysalin®, was synthesized and purified (GMP manufactured >96% purity) by American Peptide Company (Sunnyvale, CA) and provided by Chrysalis BioTherapeutics, Inc. (Galveston, TX).