Fly heads were collected from flies anaesthetized by CO2 and fixed immediately with 4% paraformaldehyde in PBS overnight at 4°C. Heads were embedded in paraffin wax, and 5-µm sections were made by microtome sectioning. Sections were then mounted on slides and stained by H&E staining. Sections were imaged on a Leica Axioplan 206 microscope using a 5× 0.15 NA Plan-Neofluar objective. Scanning electron microscopy (SEM) was performed on fly heads that were fixed in glutaraldehyde/paraformaldehyde, washed, and then fixed again with 1% osmium tetroxide. Samples were then dehydrated in gradually increasing concentrations of ethanol with a final dehydrant of hexamethyldiasilazane and air-dried before being imaged on a Hitachi TM-1000 tabletop scanning electron microscope.
Tm 1000 tabletop
Manufactured by Hitachi
The TM-1000 is a tabletop microscope designed by Hitachi. It is a versatile instrument that allows for the observation and analysis of various samples. The TM-1000 is capable of providing high-resolution images and delivering essential data for research and industrial applications.
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Lab products found in correlation
3 protocols using tm 1000 tabletop
1
Fly Head Histology and SEM
2
Microscopic Analysis of C. clariventris Nests
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Parts from three C. clariventris nests (≈0.5 dm3) were collected and transported to the laboratory and then frozen. Pieces of intact inner nest walls were observed using a scanning electron microscope (Hitachi TM‐1000 Tabletop).
3
Comprehensive Characterization of Mordenite Samples
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X-ray diffraction was performed in a Philips diffractometer X′ PERT using the radiation CuKα (λ = 0.154 nm). The percentage of mordenite present in the samples was calculated by measuring the area of the main mordenite peaks concerning the total area of the diffractogram of each sample. Nitrogen adsorption–desorption isotherms were measured at −196 °C using the micrometrics ASAP 2420 and BET calculation. Scanning electron microscopy studies were performed on a Hitachi TM-1000 Tabletop equipped with EDX. In these cases, the samples were observed without coating. A higher resolution SEM was employed to image synthetic mordenite. A Philips XL30 S-FEG was employed using Cr coating of the sample (Quorum. model Q150T s). X-ray fluorescence was carried out using a Philips WDXRF spectrometer (PW 1404) equipped with a collimator to reduce the angle of divergence of the x-rays. A Perkin-Elmer TGA7 thermobalance was used for the thermogravimetric analyses in a range of 25 to 900 °C using a heating ramp of 20 °C/min in an atmosphere of air.
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