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L165041

Manufactured by Bio-Techne
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Sourced in United Kingdom

The L165041 is a lab equipment product manufactured by Bio-Techne. It is a device designed for use in laboratory settings, but the core function of the product is not available in a concise, unbiased, and factual manner without extrapolation.

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Lab products found in correlation

Gw0742, by Bio-Techne (2 mentions) Hepg2 cell, by American Type Culture Collection (2 mentions) Hek293a cell line, by Thermo Fisher Scientific (1 mentions) Aml12 cells, by American Type Culture Collection (1 mentions) Wy14643, by Merck Group (1 mentions) Ifn β, by BioLegend (1 mentions) Lps ek, by InvivoGen (1 mentions) Legendplex mouse inflammation panel, by BioLegend (1 mentions) Tnf α, by BioLegend (1 mentions) Poly 1 c, by Merck Group (1 mentions) Ifn γ, by BioLegend (1 mentions) Anti ldlr antibody, by Abcam (1 mentions) Actinomycin d, by Merck Group (1 mentions) Anti β actin, by Merck Group (1 mentions)

3 protocols using l165041

1

Comparative Analysis of Hepatic Cell Lines

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Human hepatoma HepG2 cells and mouse hepatoma AML12 cells were obtained from ATCC. HEK293A cell line was obtained from Invitrogen. Hamster primary hepatocytes were isolated from male golden Syrian hamsters in our laboratory. Human primary hepatocytes were obtained from Invitrogen. Mouse primary hepatocytes were isolated from male C57BL/6 J mice at San Francisco General Hospital Liver Center. L165041 and GW0742 were purchased from TOCRIS Biosciences (Bristol, UK). Cholesterol, 25-hydroxylcholesterl and WY-14643 were purchased from Sigma, and 6α-ethyl-chenodeoxycholic acid (6ECDCA) and 24(S)-hydroxylcholesterol (24-OHC) were purchased from Abcam (Cambridge, MA).
Kan C.F., Singh A.B., Dong B., Shende V.R, & Liu J. (2015). PPARδ activation induces hepatic long-chain acyl-CoA synthetase 4 expression in vivo and in vitro. Biochimica et biophysica acta, 1851(5), 577-587.
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2

TLR Ligand Stimulation of Immune Cells

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50,000–100,000 pMACs or 100,000 BMDCs or RAW264.7 cells were plated in 100 μl complete media in 96 well plates. Cells were stimulated with the TLR ligands LPS (derived from Escherichia coli K12; LPS-EK, Invivogen), PolyI:C (Sigma), CpG (class C ODN 2395, Miltenyi) or alive E. coli (DH5α) at the indicated concentrations. Where stated, cells were cultured overnight with 25 μM SSO (Abcam), 5 μg/ml CD36 blocking antibody (JC63.1, Abcam), 2 μg/ml αCD1d (19G11, InVivoMab) or isotype control, or 10 μM L165,041 (Tocris) prior to stimulation. BMDCs were also stimulated for 6 h with 0.1 ng/ml IFN-β (Biolegend), 0.05 ng/ml TNF-α (Biolegend) or 20 ng/ml IFN-γ (Biolegend). Cytokines were measured in the supernatant of the culture by cytometric bead array (LEGENDplex™ Mouse Inflammation Panel, Biolegend) and mRNA was measured by qPCR.
Brailey P.M., Evans L., López-Rodríguez J.C., Sinadinos A., Tyrrel V., Kelly G., O’Donnell V., Ghazal P., John S, & Barral P. (2022). CD1d-dependent rewiring of lipid metabolism in macrophages regulates innate immune responses. Nature Communications, 13, 6723.
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3

Hepatocyte Isolation and Cell Culture

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Human hepatoma HepG2 cells were obtained from ATCC. L165041 and GW0742 were purchased from TOCRIS Biosciences (Bristol, UK). Actinomycin D and anti-β-actin were purchased from Sigma. Anti-LDLR antibody was obtained from BioVision (Mountain View, CA). Mouse primary hepatocytes were isolated from male C57BL/6J mice at San Francisco General Hospital Liver Center.
Shende V.R., Singh A.B, & Liu J. (2015). A Novel Peroxisome Proliferator Response Element Modulates Hepatic Low Density Lipoprotein Receptor Gene Transcription in Response to PPARδ Activation. The Biochemical journal, 472(3), 275-286.
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