TNF‐α (H052), IL‐1β (H002), IL‐6 (H007), and IL‐18 (H015) levels from mice kidney tissue and HK‐2 cells were measured using inflammation ELISA Kits (Nanjing Jiancheng Bioengineering Research Institute Co., Ltd, Nanjing, China). MDA (A003‐1‐2), SOD (A001‐3‐2), GSH (A006‐2‐1), and GSH‐PX (A005‐1‐2) levels from mice kidney tissue and HK‐2 cells were measured using MDA, SOD, GSH, and GSH‐PX kits according to the methods of manufacturer instructions (Nanjing Jiancheng Bioengineering Research Institute Co., Ltd, Nanjing, China). Absorbance was measured using UniCel DxC 600 Chemistry Analyzer (Beckman Coulter, CA, USA). ROS production from HK‐2 cells was assessed using an Olympus FluoView1000 confocal laser scanning microscope (using Ex/Em λ = 480 nm/535 nm).
Unicel dxc 600 chemistry analyzer
Manufactured by Beckman Coulter
Sourced in United States
The UniCel DxC 600 Chemistry Analyzer is a diagnostic instrument designed for automated clinical chemistry analysis. It performs a variety of routine and specialized tests on patient samples to aid in medical diagnosis and treatment monitoring.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using unicel dxc 600 chemistry analyzer
1
Inflammatory and Oxidative Markers in Kidney Tissue and Cells
2
Metabolic Profiling of Mouse Urine and Plasma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were placed in a 24-hours metabolic cage for urine collection and urinary albumin levels were measured by a mouse albumin ELISA kit (Bethyl Laboratories, Montgomery, TX, USA). Urinary creatinine levels were assayed by a Creatinine Assay Kit (DICT-500; BioAssay Systems, CA, USA). The ratio of urinary albumin to creatinine was calculated to correct the difference in urine volume and expressed in micrograms per milligram. Before being killed, blood of mice was collected through retro-orbital vein under avertin anesthesia. Plasma cholesterol and triglyceride levels were determined using a UniCel DxC 600 Chemistry Analyzer (Beckman Coulter, CA, USA).
3
Renal Function Evaluation in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Body weight and renal weight measurements of mice were accurate to 0.01 g with electronic scales. Kidney-to-body weight ratio was calculated as left or right kidney weight (g) divided by the body weight (kg). After anesthesia with avertin, heparin-anticoagulated blood of mice was collected from the angular vein. BUN, TCHO, and triglyceride levels in plasma were determined with a UniCel DxC 600 Chemistry Analyzer (Beckman Coulter). 24 hours’ urine was collected in the metabolic cage, and urinary albumin concentration was measured by a mouse albumin ELISA kit (Bethyl Laboratories). Urinary creatinine level was measured by a Creatinine Assay Kit (DICT-500; BioAssay Systems). uACR was calculated as urinary albumin (μg) divided by urinary creatinine (mg).
4
Biomarkers of Inflammation and Tissue Damage
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Laboratory values (concentration of leukocytes, C-reactive protein (CRP), lactate dehydrogenase (LDH), creatine phosphokinase (CPK), and aspartate aminotransferase (AST)) were regarded as surrogate markers for inflammation and tissue destruction. The values of blood samples obtained from the day of bacterial sampling were observed. If no blood had been taken on the day of bacterial sampling, data from samples obtained on the preceding or the following days were used, a maximum of three days before or after bacterial sampling. The concentrations of CRP, LDH, CPK, and AST were determined using the Alinity analyzer (Abbott, Abbott Park, IL, USA) whereas the concentration of leukocytes was measured with the UniCel DxC 600 Chemistry Analyzer (Beckman Coulter Inc., Brea, CA, USA). The laboratory of Ingolstadt Hospital is certified in accordance with the national accreditation body of the Federal Republic of Germany (Deutsche Akkreditierungsstelle; DAkkS; registration number D-ML-19856-01-00).
5
Serum Isolation and Liver Enzymes Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood was collected immediately after decapitation and serum was separated by centrifugation at 6000× g for 15 min (MIKRO 200R, Hettich®), and the supernatant was kept at −80 °C until analysis. AST and ALT activities were determined according to the International Federation of Clinical Chemistry [31 (link)] procedure with the Beckman Coulter UniCel® DxC600 Chemistry Analyzer.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!