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Rabbit anti ser505 phospho dakt

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Rabbit anti-Ser505-phospho-dAKT is a primary antibody that specifically recognizes the phosphorylated form of Drosophila AKT (dAKT) at serine 505. It can be used to detect and quantify the levels of phosphorylated dAKT in various applications such as Western blotting and immunohistochemistry.

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Lab products found in correlation

Rabbit anti akt, by Cell Signaling Technology (3 mentions) Polyacrylamide gel, by Bio-Rad (3 mentions) Pvdf membrane, by Bio-Rad (3 mentions) Sds page sample buffer, by Bio-Rad (3 mentions) P0191, by Teknova (3 mentions) Hybridoma bank ab 579794, by Developmental Studies Hybridoma Bank (3 mentions) Anti mouse and anti rabbit igg horseradish peroxidase conjugated secondary antibodies, by Cell Signaling Technology (3 mentions)

3 protocols using rabbit anti ser505 phospho dakt

1

Quantifying Drosophila AKT Signaling

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Ten whole female flies (5–7 days old) were homogenized in PBS (Teknova P0191). Protein samples were reduced and denatured in SDS-PAGE sample buffer (BioRad 161–0747) for 20 min 95°C, separated on a 4–20% polyacrylamide gel (BioRad 456–1096) and transferred to a PVDF membrane (BioRad 1704272). Extracts were immunoblotted with rabbit anti-AKT (1:1000, Cell Signaling Technologies 9272), rabbit anti-Ser505-phospho-dAKT (1:1000, Cell Signaling Technologies 4054), mouse anti-β-Tubulin (1:2500, Developmental Studies Hybridoma Bank AB_579794), anti-mouse and anti-rabbit IgG horseradish peroxidase-conjugated secondary antibodies (1:5000, Cell Signaling Technologies, 7074 and 7072, respectively).
Kamareddine L., Robins W.P., Berkey C.D., Mekalanos J.J, & Watnick P.I. (2018). The Drosophila immune deficiency pathway modulates enteroendocrine function and host metabolism. Cell metabolism, 28(3), 449-462.e5.
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2

Quantifying Drosophila AKT Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ten whole female flies (5–7 days old) were homogenized in PBS (Teknova P0191). Protein samples were reduced and denatured in SDS-PAGE sample buffer (BioRad 161–0747) for 20 min 95°C, separated on a 4–20% polyacrylamide gel (BioRad 456–1096) and transferred to a PVDF membrane (BioRad 1704272). Extracts were immunoblotted with rabbit anti-AKT (1:1000, Cell Signaling Technologies 9272), rabbit anti-Ser505-phospho-dAKT (1:1000, Cell Signaling Technologies 4054), mouse anti-β-Tubulin (1:2500, Developmental Studies Hybridoma Bank AB_579794), anti-mouse and anti-rabbit IgG horseradish peroxidase-conjugated secondary antibodies (1:5000, Cell Signaling Technologies, 7074 and 7072, respectively).
Kamareddine L., Wong A.C., Vanhove A.S., Hang S., Purdy A.E., Pearson K.K., Asara J.M., Ali A., Morris JG J.r, & Watnick P.I. (2017). Activation of Vibrio cholerae quorum sensing promotes survival of an arthropod host. Nature microbiology, 3(2), 243-252.
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3

Quantifying Drosophila AKT Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ten whole female flies (5–7 days old) were homogenized in PBS (Teknova P0191). Protein samples were reduced and denatured in SDS-PAGE sample buffer (BioRad 161–0747) for 20 min 95°C, separated on a 4–20% polyacrylamide gel (BioRad 456–1096) and transferred to a PVDF membrane (BioRad 1704272). Extracts were immunoblotted with rabbit anti-AKT (1:1000, Cell Signaling Technologies 9272), rabbit anti-Ser505-phospho-dAKT (1:1000, Cell Signaling Technologies 4054), mouse anti-β-Tubulin (1:2500, Developmental Studies Hybridoma Bank AB_579794), anti-mouse and anti-rabbit IgG horseradish peroxidase-conjugated secondary antibodies (1:5000, Cell Signaling Technologies, 7074 and 7072, respectively).
Kamareddine L., Wong A.C., Vanhove A.S., Hang S., Purdy A.E., Pearson K.K., Asara J.M., Ali A., Morris JG J.r, & Watnick P.I. (2017). Activation of Vibrio cholerae quorum sensing promotes survival of an arthropod host. Nature microbiology, 3(2), 243-252.
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