Mir 505 3p

Manufactured by GenePharma

Sourced in China

MiR-505-3p is a microRNA (miRNA) product offered by GenePharma. miRNAs are small, non-coding RNA molecules that play a role in gene expression regulation. The core function of MiR-505-3p is to provide a specific miRNA sequence for research purposes.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Pcdna3, by GenePharma (1 mentions) Mir 129 5p mimic, by GenePharma (1 mentions) Mir 410 3p, by GenePharma (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions)

2 protocols using mir 505 3p

Knockdown and overexpression of gene targets

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The short hairpin RNA (shRNA) against KTN1-AS1 (sh-KTN1-AS1#1/2) or CBP (sh-CBP#1/2), miR-505-3p mimics, and their relative reference (sh-NC and NC mimics) were synthesized by GenePharma (Shanghai, China). To overexpress ZNF326, pcDNA3.1/ZNF326 (GenePharma, Shanghai, China) was synthesized, and the empty pcDNA3.1 vector was taken as an internal reference. Cell transfection was performed through Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). Forty-eight hours later, transfection efficiency was proved by RT-qPCR.

Xie X., Wen Q., Yang X., Chen W., Liu Y., Liu W., Zhang T., Xu C, & Shi K. (2022). H3K27ac-activated lncRNA KTN1-AS1 aggravates tumor progression by miR-505-3p/ZNF326 axis in ovarian cancer. Annals of Translational Medicine, 10(10), 599.

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Chondrocyte Overexpression and Inflammation

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For overexpression, miR-410-3p, miR-505-3p, and miR-129-5p mimics (GenePharma) and negative control vectors were added to the culture medium for transfection of cultured chondrocytes using Lipofectamine™ 2000 (Invitrogen). 24 h later, cells were treated with 100 ng/ml lipopolysaccharide (LPS) (Sigma) for 6 h to induce inflammation.

Pan H., Dai H., Wang L., Lin S., Tao Y., Zheng Y., Jiang R., Fang F, & Wu Y. (2020). MicroRNA-410-3p modulates chondrocyte apoptosis and inflammation by targeting high mobility group box 1 (HMGB1) in an osteoarthritis mouse model. BMC Musculoskeletal Disorders, 21, 486.

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