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Rabbit anti sin3a

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Rabbit anti-Sin3A is a primary antibody that specifically binds to the Sin3A protein. Sin3A is a transcriptional regulator that is involved in gene expression and chromatin remodeling. This antibody can be used for applications such as Western blotting and immunoprecipitation to detect and study the Sin3A protein.

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Lab products found in correlation

Streptavidin dylight650, by Thermo Fisher Scientific (2 mentions) Streptavidin dylight 800, by Thermo Fisher Scientific (2 mentions) Anti mouse irdye 800cw, by LI COR (2 mentions) Odyssey infrared imaging system, by LI COR (2 mentions) Rabbit anti histone h3, by Abcam (2 mentions) Rabbit anti tbp, by Cell Signaling Technology (2 mentions) Rabbit anti hdac3, by Santa Cruz Biotechnology (2 mentions) Anti rabbit irdye 680lt, by LI COR (2 mentions) Mab377, by Abcam (2 mentions)

2 protocols using rabbit anti sin3a

1

Quantitative Western Blot Analysis

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Quantitative Western blot was performed using Odyssey Infrared Imaging System (Licor). Primary antibodies used in this study include rabbit anti-MeCP2 C-terminus (1:4000, in house), mouse anti-MeCP2 N-terminus (1:4000, Sigma M7433), mouse anti-NeuN (1:500, Millipore MAB377), rabbit anti-Avi tag (1:5000, Abcam ab106159, listed as anti-Tavi in main text, detects the minimal peptide substrate of biotin ligase BirA regardless of biotinylation status), rabbit anti-HDAC3 (1:1000, Santa Cruz sc-11417), rabbit anti-TBLR1 (1:1000, Bethyl A300–408A), rabbit anti-Sin3A, (1:500, Thermo Scientific PA1-870), rabbit anti-Histone H3 (1:1000, Abcam ab1791), and rabbit anti-TBP (1:1000, Cell Signaling #8515). Secondary antibodies include anti-rabbit IRDye 680LT (1:10,000, Licor), anti-mouse IRDye 800CW (Licor), Streptavidin Dylight 650 (1:10,000, Fisher 84547) and Streptavidin Dylight 800 (1:10,000, Fisher 21851). Quantification of protein expression levels was carried out following Odyssey Infrared Imaging System protocols. Scans of full-length Western blot membranes are provided in Supplementary Figs. 11–13.
Johnson B.S., Zhao Y.T., Fasolino M., Lamonica J.M., Kim Y.J., Georgakilas G., Wood K.H., Bu D., Cui Y., Goffin D., Vahedi G., Kim T.H, & Zhou Z. (2017). Biotin tagging of MeCP2 in mice reveals contextual insights into the Rett syndrome transcriptome. Nature medicine, 23(10), 1203-1214.
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2

Quantitative Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantitative Western blot was performed using Odyssey Infrared Imaging System (Licor). Primary antibodies used in this study include rabbit anti-MeCP2 C-terminus (1:4000, in house), mouse anti-MeCP2 N-terminus (1:4000, Sigma M7433), mouse anti-NeuN (1:500, Millipore MAB377), rabbit anti-Avi tag (1:5000, Abcam ab106159, listed as anti-Tavi in main text, detects the minimal peptide substrate of biotin ligase BirA regardless of biotinylation status), rabbit anti-HDAC3 (1:1000, Santa Cruz sc-11417), rabbit anti-TBLR1 (1:1000, Bethyl A300–408A), rabbit anti-Sin3A, (1:500, Thermo Scientific PA1-870), rabbit anti-Histone H3 (1:1000, Abcam ab1791), and rabbit anti-TBP (1:1000, Cell Signaling #8515). Secondary antibodies include anti-rabbit IRDye 680LT (1:10,000, Licor), anti-mouse IRDye 800CW (Licor), Streptavidin Dylight 650 (1:10,000, Fisher 84547) and Streptavidin Dylight 800 (1:10,000, Fisher 21851). Quantification of protein expression levels was carried out following Odyssey Infrared Imaging System protocols. Scans of full-length Western blot membranes are provided in Supplementary Figs. 11–13.
Johnson B.S., Zhao Y.T., Fasolino M., Lamonica J.M., Kim Y.J., Georgakilas G., Wood K.H., Bu D., Cui Y., Goffin D., Vahedi G., Kim T.H, & Zhou Z. (2017). Biotin tagging of MeCP2 in mice reveals contextual insights into the Rett syndrome transcriptome. Nature medicine, 23(10), 1203-1214.
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