The immunofluorescence analysis was performed as previously described28 (link). Rabbit anti-Collagen I and mouse anti-α-SMA were employed as the primary antibodies. Alexa Fluor 594-conjugated goat anti-mouse/rabbit IgG (Invitrogen) was used as the secondary antibody. Nuclei were stained with 1 μg/ml 4',6-diamidino-2-phenylindole (DAPI; Sigma). The images were captured using a confocal fluorescence microscope (Olympus, Tokyo, Japan).
Alexa fluor 594 conjugated goat anti mouse rabbit igg
Manufactured by Thermo Fisher Scientific
Alexa Fluor 594-conjugated goat anti-mouse/rabbit IgG is a secondary antibody conjugated with the Alexa Fluor 594 fluorescent dye. It is designed to detect and visualize primary antibodies raised in mouse or rabbit.
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2 protocols using alexa fluor 594 conjugated goat anti mouse rabbit igg
1
Immunofluorescence Analysis of Collagen I and α-SMA
2
Immunofluorescence staining of brain tissue
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Frozen sections were washed with 0.01 M PBS for 10 minutes and then treated with 0.3% Triton X-100 at RT for 20 minutes. After blocking with 10% normal goat serum at 37°C for 30 minutes, the samples were incubated with primary antibodies (MAP2, GALC, GFAP, PSD95, and SYP) overnight at 4°C under humidified conditions and then incubated with Alexa Fluor 594-conjugated goat anti-mouse/rabbit IgG (1 : 500, Invitrogen) at RT for 60 min. Finally, sections were stained with 4′,6-diamidino-2-phenylindole (DAPI) and covered with fluorescence mounting medium (Dako).
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