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Anti vcam1 429

Manufactured by BD

Anti-VCAM1 (429) is a laboratory reagent used for research purposes. It functions as an antibody that specifically binds to the Vascular Cell Adhesion Molecule 1 (VCAM-1) protein.

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2 protocols using anti vcam1 429

1

Immunohistochemical Staining of Cryosections

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Cryosections 7um in thickness were cut and fixed in cold acetone.
Sections were stained with anit-ICAM1 (3E2; BD Biosciences); anti-VCAM1 (429; BD
Biosciences); anti-MADCAM1(MECA367; Bio X Cell); anti-IgD (11-26c.2a;
Biolegend); anti-Vitronectin (347317; R&D); rat IgG2a isotype control
(2A3; Bio X Cell) or anti-CD35 (8C12; BD Biosciences) using described protocols
(36 (link)).
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2

Antigen-Specific Humoral Immunity Modulation

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Mice were injected intraperitoneally with 50 μg TNP-LPS, 50 μg NP-Ficoll, or 150 μg adsorbed NP-KLH (Biosearch Technology) 1:1 ratio onto Alu-Gel-S (Serva). Spleens, BM, and blood were taken after the indicated time points postimmunization. ASCs were analyzed by ELISpot as previously described (13 (link)). In some experiments, mice received 100 μg of anti–VCAM-1 (429) or rat IgG2a isotype control antibodies (both BD), intravenously, 24 h postinfection. Spleens and BM were analyzed 48 h after antibody treatment.
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