Mice were injected intraperitoneally with 50 μg TNP-LPS, 50 μg NP-Ficoll, or 150 μg adsorbed NP-KLH (Biosearch Technology) 1:1 ratio onto
Alu-Gel-S (Serva). Spleens, BM, and blood were taken after the indicated time points postimmunization. ASCs were analyzed by ELISpot as previously described (13 (
link)). In some experiments, mice received 100 μg of
anti–VCAM-1 (429) or rat IgG2a isotype control antibodies (both BD), intravenously, 24 h postinfection. Spleens and BM were analyzed 48 h after antibody treatment.
Andreani V., Ramamoorthy S., Pandey A., Lupar E., Nutt S.L., Lämmermann T, & Grosschedl R. (2018). Cochaperone Mzb1 is a key effector of Blimp1 in plasma cell differentiation and β1-integrin function. Proceedings of the National Academy of Sciences of the United States of America, 115(41), E9630-E9639.