All experiments were performed in accordance with the NIH and the University of Florida guidelines for the care and use of animals for experimental procedures, and the protocols were approved by University of Florida’s Institutional Animal Care and Use Committee. Adult male C57BL/6J (3–3.5 months old; 25.0 ± 2.0g) mice from our breeding facility were used in this study. ET-1 or L-NIO (Enzo Life Science Inc., Farmingdale, NY) were freshly prepared in saline (vehicle).
L nio
Manufactured by Enzo Life Sciences
L-NIO is a laboratory reagent used for inhibiting nitric oxide synthase (NOS) activity. It is a potent and selective inhibitor of endothelial NOS. L-NIO is commonly used in scientific research to study the role of nitric oxide in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Protease inhibitor tablet, by Roche (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Calcium green 1 am, by Thermo Fisher Scientific (1 mentions)
L arginine, by Merck Group (1 mentions)
Wortmannin, by Santa Cruz Biotechnology (1 mentions)
Bradykinin, by Abcam (1 mentions)
2 protocols using l nio
1
Investigating Vascular Effects in Mice
2
Comprehensive Cell Culture Protocol
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Dulbecco's modi ed Eagle's medium (DMEM) is purchased from PAN Biotech and Foetal Bovine Serum (FBS) from Invitrogen Life Technologies (Gaithersburg, MD). Human recombinant leptin, Bradykinin, Phenylmethylsulfonyal uoride (PMSF), Picoll, 5Fluorouracil (5FU), 5-Chloromethyl ouorescein diacetate (CMFDA), and all the primary antibodies are purchased from Abcam, USA. Propidium Iodide (PI), L-NAME, L-Arginine, 4',6-diamidino-2-phenylindole dihydrochloride (DAPI), recombinant VEGF were from Sigma Chemical Co (St. Louis, MO). L-NIO is purchased from Enzo life sciences and Wortmannin from Santa Cruz Biotechnologies. Collagen type-1 purchased from Pan Biotech GmbH Am Gewerbeperk. DAF-FM (4 amino-5-methylamino-2'7'di uoroscein) and Calcium green1/AM were from Molecular probes, Eugene, Oregon, USA. Protease inhibitor tablets from Roche Diagnostics. All the primary antibodies are purchased from Abcam, and all the secondary antibodies and DAB systems are purchased from Bangalore Gene, India. All the other chemicals are at least of the reagent grade and are obtained commercially.
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