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Znf259 sirna sc 35282

Manufactured by Santa Cruz Biotechnology
Sourced in United States

ZNF259-siRNA (sc-35282) is a short interfering RNA (siRNA) targeting the ZNF259 gene. This product is designed for the specific knockdown of ZNF259 gene expression.

Automatically generated - may contain errors

2 protocols using znf259 sirna sc 35282

1

Plasmid Transfection and siRNA Silencing

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Plasmids pCMV6-ddk-myc and pCMV6-ddk-myc-ZNF259 were purchased from Origene (RC205721; Origene, Rockville, MD, USA). ZNF259-siRNA (sc-35282) and negative control-siRNA (sc-37007) were purchased from Santa Cruz Biotechnology. Transfection was carried out using the Lipofectamine 3000 reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions. Cells were harvested within 48 h after plasmid transfection. For siRNA treatment, cells were harvested after 72 h.
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2

Plasmid Transfection Protocol

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Plasmids pCMV6-ddk-myc and pCMV6-ddk-myc-ZNF259 were purchased from Origene (RC205721, Rockville, MD, USA). ZNF259-siRNA (sc-35282) and NC-siRNA (sc-37007) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Transfection was carried out using the Lipofectamine 3000 reagent (Invitrogen) according to the manufacturer’s instructions.
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