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4 protocols using e e 2 4 hexadienal

1

Retention Index Determination of Volatile Compounds

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A mixture of n-alkanes (C8—C20) dissolved in hexane for retention index determination was purchased from Fluka (Buchs, Switzerland). Reference compounds, (E)-2-hexen-1-ol (> 96%), (E)-2-octen-1-ol (> 97%), 1-decyne (> 98%), 1-dodecene (≥ 99%), (E,E)-2,4-hexadienal (≥ 97%), 2-cyclohexen-1-ol (> 95%), (E)-2-hexenal (≥ 99%), (E)-2-octenal (≥ 95%), 4-tert-butylcyclohexyl acetate (mixture of E and Z, > 98%), allyl cyclohexanepropionate (≥ 98%), cyclopentanemethanol (≥ 98%), cyclopentanol (≥ 99%), cyclopentanone (≥ 99%), decanal (≥ 98%), 1-dodecanol (≥ 98%), dodecyl acetate (> 97%), hexyl acetate (≥ 99%), hexyl salicylate (≥ 99%), limonene (> 97%), nonanal (≥ 97%), octyl acetate (≥ 99%), p-cymene (> 99%), 1-tridecanol (≥ 99%), and α-hexylcinnamaldehyde (≥ 95%) were supplied by Sigma Aldrich (Munich, Germany).
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2

Volatile Compounds in Food Analysis

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The following compounds (food grade, purity in parenthesis) were purchased from Sigma-Aldrich (St. Louis, MO, USA): ethyl acetate (≥99%), ethanol (96%), hexanal (≥97%), 4-methylpentan-2-ol (98%), E-2-hexenal (≥95%), hexan-1-ol (≥98%), E-3-hexen-1-ol (97%), E,E-2,4-hexadienal (95%), nonanal (≥98%), acetic acid (≥99.5%), E-2-decenal, pentanoic acid (≥99%), hexanoic acid (≥98%), and E,E-2,4-decadienal (≥89%).
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3

Volatile Compound Identification Protocol

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The standard compounds employed in this study for identification and quantification were supplied by Sigma-Aldrich (Darmstadt, Germany), including hexanal, (E)-2-hexenal, nonanal, (E,E)-2,4-hexadienal, (E)-2-nonenal, benzaldehyde, pentanol, hexanol, (Z)-3-hexen-1-ol, (E)-2-hexen-1-ol, 2-ethyl-1-hexanol, nonanol, benzyl alcohol, 2-phenylethanol, limonene, α-ocimene, linalool, citronellol, nerol, geraniol, and isovalerone. Sodium chloride and 4-methyl-2-pentanol (internal standard) were purchased from Merck (Darmstadt, Germany).
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4

Characterization of Volatile Organic Compounds

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Deionized water was produced using a Milli-Q water purification system (Millipore, Billerica, MA, USA). N-alkanes (C7–C40) and 1-hexanol were obtained from the J&K Scientific Corporation (Beijing, China). (Z)-3-Hexen-1-ol, linalool, cis-linalool oxide (furanoid), trans-linalool oxide (furanoid), cis-linalool oxide (pyranoid), trans-linalool oxide (pyranoid), (E)-2-nonenal, α-ionone, β-ionone, cis-3-hexenyl isovalerate, and cis-3-hexenyl hexanoate were purchased from the TCI Corporation (Tokyo, Japan). Phenylacetaldehyde and 1-octen-3-one were obtained from the Alfa Aesar Corporation (Shanghai, China). 1-Octen-3-ol, β-farnesene, geraniol, nerolidol, (E)-2-hexenal, hexanal, octanal, (E,E)-2,4-heptadienal, melonal, (E)-2-octenal, 2,2,6-trimethylcyclohexanone, 3-octen-2-one, dihydroactinidiolide, methyl salicylate, hexyl hexanoate, and 1-ethyl-1H-pyrrole-2-carboxaldehyde were obtained from the Aladdin Corporation (Shanghai, China). (E,E)-2,4-Hexadienal, β-damascenone, and β-octalactone were purchased from the Sigma-Aldrich Corporation (Shanghai, China).
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