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M165 fc stereomicroscope

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The Leica M165 FC stereomicroscope is a high-performance, versatile imaging instrument. It provides a magnification range of 7.8x to 160x, enabling detailed observation and analysis of a wide variety of specimens. The M165 FC features a fully apochromatic optical system, ensuring accurate color reproduction and high-resolution imaging. Its ergonomic design and intuitive controls make it a reliable tool for various laboratory applications.

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Lab products found in correlation

Lsm 710, by Zeiss (4 mentions) Dfc425c camera, by Leica (3 mentions) Dfc500 12 megapixel camera, by Leica (3 mentions) Dfc450 c camera, by Leica (3 mentions) Dicerflx flx mice, by Jackson ImmunoResearch (2 mentions) Strain 00601, by Jackson ImmunoResearch (2 mentions) Stemi 2000 microscope, by Zeiss (2 mentions) Feratrack mri spio nanoparticles, by Miltenyi Biotec (2 mentions) Feratrack spio, by Miltenyi Biotec (2 mentions) Purecol ez gel type 1 collagen solution, by Advanced BioMatrix (2 mentions) Prussian blue iron assay kit, by Merck Group (2 mentions) H7904, by Merck Group (2 mentions) Cryostar nk70, by Thermo Fisher Scientific (2 mentions) Ix81 microscope, by Hamamatsu Photonics (2 mentions) Dmi6000 microscope, by Leica (2 mentions) Dfc425c digital camera, by Leica (2 mentions) Rm2035, by Leica (2 mentions) Dfc290 color digital camera, by Leica (2 mentions) Alkaline phosphatase conjugated anti dig antibody, by Roche (2 mentions) Alcian blue, by Merck Group (2 mentions)

Close spelling variants of this product

M165 FC (513 citations) Stereomicroscope (344 citations) M165 FC fluorescence stereomicroscope (14 citations) M165 stereomicroscope (12 citations) M165 FC epifluorescence stereomicroscope (2 citations)

88 protocols using m165 fc stereomicroscope

1

Conditional Dicer Knockout in Tbx18-Expressing Lineages

Cited 2 times
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Tbx18CreTg/+ mice that drive Cre recombinase expression in the peri-Wolffian duct stroma (gift from Feng Chen) [23 (link)] were bred with Dicerflx/flx mice ([24 (link)], Jackson Laboratories, Strain #00601, Bar Harbor, ME) to generate conditional knockout mice with loss of Dicer in the Tbx18-expressing lineages. Tbx18CreTg/+;Dicerflx/flx (mutant mice) were compared to littermates without the Tbx18Cre allele (controls). To detect Cre expression, Tbx18CreTg/+ mice were bred with Gt(ROSA)26Sortm9(CAG-tdTomato)hZE (CAG) mice ([25 (link)], Jackson Laboratories, Strain #007909) to generate Tbx18CreTg/+CAG offspring in which tdTomato fluorescence is expressed in lineages arising from Tbx18Cre-positive cells. Timed matings were performed; the morning a plug was observed was considered embryonic day 0.5. We detected red fluorescence from tdTomato positive mice with a Leica M165FC Stereo Microscope (Leica, Buffalo Grove, IL). DNA was isolated from tail clippings or embryonic tissue to perform polymerase chain reaction (PCR) genotyping. All animal experiments were carried out in accordance with the policies of the Institutional Animal Care and Use Committee at the University of Pittsburgh School of Medicine (Protocol #17091432). Animals were housed in the vivarium at Rangos Research Center at the UPMC Children’s Hospital of Pittsburgh, Pittsburgh, Pennsylvania.
Anslow M.J., Bodnar A.J., Cerqueira D.M., Bushnell D., Shrom B.E., Sims-Lucas S., Bates C.M, & Ho J. (2020). Increased rates of vesicoureteral reflux in mice from deletion of Dicer in the peri-Wolffian duct stroma. Pediatric research, 88(3), 382-390.
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2

Conditional Dicer Knockout in Tbx18-Expressing Lineages

Cited 2 times
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Tbx18CreTg/+ mice that drive Cre recombinase expression in the peri-Wolffian duct stroma (gift from Feng Chen) [23 (link)] were bred with Dicerflx/flx mice ([24 (link)], Jackson Laboratories, Strain #00601, Bar Harbor, ME) to generate conditional knockout mice with loss of Dicer in the Tbx18-expressing lineages. Tbx18CreTg/+;Dicerflx/flx (mutant mice) were compared to littermates without the Tbx18Cre allele (controls). To detect Cre expression, Tbx18CreTg/+ mice were bred with Gt(ROSA)26Sortm9(CAG-tdTomato)hZE (CAG) mice ([25 (link)], Jackson Laboratories, Strain #007909) to generate Tbx18CreTg/+CAG offspring in which tdTomato fluorescence is expressed in lineages arising from Tbx18Cre-positive cells. Timed matings were performed; the morning a plug was observed was considered embryonic day 0.5. We detected red fluorescence from tdTomato positive mice with a Leica M165FC Stereo Microscope (Leica, Buffalo Grove, IL). DNA was isolated from tail clippings or embryonic tissue to perform polymerase chain reaction (PCR) genotyping. All animal experiments were carried out in accordance with the policies of the Institutional Animal Care and Use Committee at the University of Pittsburgh School of Medicine (Protocol #17091432). Animals were housed in the vivarium at Rangos Research Center at the UPMC Children’s Hospital of Pittsburgh, Pittsburgh, Pennsylvania.
Anslow M.J., Bodnar A.J., Cerqueira D.M., Bushnell D., Shrom B.E., Sims-Lucas S., Bates C.M, & Ho J. (2020). Increased rates of vesicoureteral reflux in mice from deletion of Dicer in the peri-Wolffian duct stroma. Pediatric research, 88(3), 382-390.
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3

Maize Root Histological Analysis

Cited 1 time
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The root tips of maize primary roots were collected following salt treatment for 4 d and fixed in a 50% formalin-acetic acid-alcohol (FAA) fixative solution, before being embedded in paraffin as previously described (Qin et al., 2017 (link)). The paraffin sections of 10 µm thickness were prepared using a Leica RM2265 microtome (Leica Biosystems, Nussloch, Germany) and placed on glass slides. After staining with toluidine blue, the sections were observed and imaged using a Leica M165FC stereo microscope (Leica Biosystems, Nussloch GmbH, Germany).
Xing L., Zhang L., Zheng H., Zhang Z., Luo Y., Liu Y, & Wang L. (2023). ZmmiR169q/ZmNF-YA8 is a module that homeostatically regulates primary root growth and salt tolerance in maize. Frontiers in Plant Science, 14, 1163228.
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4

Atherosclerotic Plaque Quantification in Mice

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The procedures used were adopted from the Eitzman Lab (29 (link)). Apoe−/− mice were perfused with saline at physiological pressure and then fixed using formalin with a 25-gauge needle inserted into the left ventricle at a rate of 1 ml/min. The arterial tree was then meticulously dissected and placed in 70% ethanol. After staining with Oil Red O, the aorta was pinned on wax. Pinned aortic tissue was imaged using a SPOT camera (Spot Imaging; Sterling Heights, MI, USA) mounted to a Leica M165FC Stereomicroscope (Leica Biosystems; Nussloch, Germany). Identical settings were used for all samples. The surface area occupied by atherosclerotic plaques was quantified at the aortic arch and major branches with Image-Pro Plus software (Media Cybernetics, Bethesda, MD, USA). The lesion area was expressed as a percentage of total surface area examined.
Shaw M.K., Tse K.Y., Zhao X., Welch K., Eitzman D.T., Thipparthi R.R., Montgomery P.C., Thummel R, & Tse H.Y. (2017). T-Cells Specific for a Self-Peptide of ApoB-100 Exacerbate Aortic Atheroma in Murine Atherosclerosis. Frontiers in Immunology, 8, 95.
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5

Multimodal Microscopy for Zebrafish and C. elegans

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For zebrafish, a Leica SP8 (Leica, Bannockburn, IL) laser scanning confocal microscope (LSCM) was used throughout manuscript. A HC PL APO 20x/0.75 IMM CORR CS2 objective, HC PL APO 40x/1.10 W CORR CS2 0.65 water immersion objective, and an HCX Plan Apochromat 63×/1.40–0.06 NA OIL objective were used. Images were acquired using LAS-X software. Images taken with the SP8 LSCM were obtained through lightning, a built-in deconvolution algorithm. A Leica DMi8 (Leica, Bannockburn, IL) with a X-light v2 confocal unit spinning disk was also used, equipped with an 89 North – LDI laser and a Photometrics Prime-95B camera. Optics used were either 10x/0.32 NA air objective, HC PL APO 63X/1.40 NA oil CS2, HC PL APO 40X/1.10 NA WCS2 CORR, a 40X/1.15 N.A. 19 Lamda S LWD, or 100Å~/1.4 N.A. HC Pl Apo oil emersion objective. A Leica M165 FC stereomicroscope equipped with DFC9000 GT sCMOS camera was used for phenotypic analysis of embryos.
For live cell imaging of C. elegans embryos, a spinning disk confocal system was used. The system is equipped with a Nikon Eclipse and is an inverted microscope with a 60X 1.40NA objective, a CSU-22 spinning disc system and a Photometrics EM-CCD camera from Visitech International. Images were obtained every 2 minutes with a 1-micron z-stack step size.
Rathbun L.I., Aljiboury A.A., Bai X., Hall N.A., Manikas J., Amack J.D., Bembenek J.N, & Hehnly H. (2020). PLK1- and PLK4-mediated asymmetric mitotic centrosome size and positioning in the early zebrafish embryo.. Current biology : CB, 30(22), 4519-4527.e3.
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6

Wholemount Confocal Imaging of Ductal Network

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For wholemount confocal imaging, samples were dissected under a Leica M165FC stereomicroscope to yield portions containing a large area of ductal network (approximately 3 mm x 3 mm). Adipose tissue was removed using dissecting scissors (Rios et al., 2014 (link)). Small pieces of tissue were cleared in 80% glycerol overnight and then covered with mounting medium.
Liu R., Hu H., McNeil M., Xu J., Bi X., Lou P., Guerrero-Juarez C.F., Dai X., Plikus M.V., Shuai J., Yu Z, & Lv C. (2022). Dormant Nfatc1 reporter-marked basal stem/progenitor cells contribute to mammary lobuloalveoli formation. iScience, 25(3), 103982.
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7

Imaging and Quantifying Hatching Gland Cells

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Images were taken using a Zeiss Confocal (LSM710 META), an AxioImager microscope (Zeiss) equipped with an Apotome, using the AxioVision software (Zeiss) or a Leica M165FC stereo microscope with DFC425C camera and the Leica Application Suite V3.8 software and processed using the ImageJ software and Adobe Photoshop. Cell shapes of hatching gland cells were determined by the shape descriptor tool in ImageJ, calculating circularity by 4π × Area/Perimeter2, and solidity by Area/Convex area.
Hatzold J., Wessendorf H., Pogoda H.M., Bloch W, & Hammerschmidt M. (2021). The Kunitz-type serine protease inhibitor Spint2 is required for cellular cohesion, coordinated cell migration and cell survival during zebrafish hatching gland development. Developmental biology, 476, 148-170.
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8

Zebrafish Embryonic Cyst Formation Analysis

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Zebrafish strains: zebrafish were maintained according to institutional and national ethical and animal welfare guidelines. All experiments were performed under UK Home Office licenses 40/3708 and 70/8588. The zebrafish lines Tg(-26wt1b:EGFP)li164 (link) and tmem33sh44343 (link) were used.
Zebrafish morpholino injections: Embryos were injected at the 1 cell stage. A morpholino complementary to the ATG region of zebrafish pkd2 was injected at 1 ng (5’-AGGACGAACGCGACTGGAGCTCATC-3’)42 (link). A concentration of 0.4 ng or 1 ng control morpholino (5’- CCTCTTACCTCAGTTACAATTTATA-3’) was injected in these experiments.
Image acquisition and analysis: Zebrafish embryos were imaged at 52–55hpf when cysts became visible, using a Leica M165FC stereo microscope and images were acquired using Leica LASX software. Images were quantified using FIJI v1.52i. Zebrafish renal phenotype analysis was double blinded.
Arhatte M., Gunaratne G.S., El Boustany C., Kuo I.Y., Moro C., Duprat F., Plaisant M., Duval H., Li D., Picard N., Couvreux A., Duranton C., Rubera I., Pagnotta S., Lacas-Gervais S., Ehrlich B.E., Marchant J.S., Savage A.M., van Eeden F.J., Wilkinson R.N., Demolombe S., Honoré E, & Patel A. (2019). TMEM33 regulates intracellular calcium homeostasis in renal tubular epithelial cells. Nature Communications, 10, 2024.
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9

Visualizing Alzheimer's in Fly Eyes

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Eye images of 6-day-old female flies expressing Aβ under the control of the GMR-Gal4 driver at 25 °C were taken using a Leica M165 FC stereo microscope. At least 8 flies per genotype were investigated.
Burnouf S., Gorsky M.K., Dols J., Grönke S, & Partridge L. (2015). Aβ43 is neurotoxic and primes aggregation of Aβ40 in vivo. Acta Neuropathologica, 130(1), 35-47.
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10

Microscopic Analysis of Pid Mutants

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The screen for Pid mutants was performed using a Zeiss M2Bio dissecting microscope. Fluorescence images were taken on a Leica DM6000 B upright microscope with 400× amplification. Silencing onset of active transgenes was analyzed using a Leica M165 FC stereo microscope with a 1× objective (120× amplification) or a 4× objective (480× amplification).
de Albuquerque B.F., Luteijn M.J., Cordeiro Rodrigues R.J., van Bergeijk P., Waaijers S., Kaaij L.J., Klein H., Boxem M, & Ketting R.F. (2014). PID-1 is a novel factor that operates during 21U-RNA biogenesis in Caenorhabditis elegans. Genes & Development, 28(7), 683-688.
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