Potassium sorbate

Manufactured by Merck Group

Sourced in United States, Germany, Portugal, Argentina

Potassium sorbate is a chemical compound used as a food preservative. It is a white, crystalline solid that is soluble in water. Potassium sorbate is commonly used to inhibit the growth of mold, yeast, and other microorganisms in a variety of food and beverage products.

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Lab products found in correlation

49 protocols using potassium sorbate

Antimicrobial Evaluation of Phenolic Compounds

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The two phenolic compounds used in the study, i.e., vanillin and cinnamic acid, and the synthetic compounds, i.e., sodium benzoate, potassium sorbate, and sodium diacetate, were purchased from Sigma-Aldrich (Munich, Germany). The phenolic compounds were selected based on a previous investigation that indicated very high antimicrobial activity of the two natural compounds against food spoilage yeasts [27 (link)]. The stock solutions of the phenolics and synthetic compounds were prepared in 10% (v/v) ethanol.

Kimani B.G., Takó M., Veres C., Krisch J., Papp T., Kerekes E.B, & Vágvölgyi C. (2023). Activity of Binary Combinations of Natural Phenolics and Synthetic Food Preservatives against Food Spoilage Yeasts. Foods, 12(6), 1338.

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Pectin-based Antioxidant Evaluation

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Deionized water (MilliQ, Millipore, USA) was used. Food grade pectin with a low degree of methyl esterification (GENU™ pectin type LM-12 CG) was a gift from CP Kelco (Denmark). Potassium sorbate, bovine serum albumin standard, D-galacturonic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-tripyridyl-s-triazine (TPTZ), and FeCl₃.6H₂O were obtained from Sigma–Aldrich (St. Louis, USA). The other chemicals were of analytical quality from Merck (Argentina).

De'Nobili M.D., Bernhardt D.C., Basanta M.F, & Rojas A.M. (2021). Sunflower (Helianthus annuus L.) Seed Hull Waste: Composition, Antioxidant Activity, and Filler Performance in Pectin-Based Film Composites. Frontiers in Nutrition, 8, 777214.

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Characterization of Swine Belly Tissue Phantom

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A homogenous custom elastic phantom and two samples of excised swine belly tissue were used. An elastic phantom with agarose concentration (by weight) of 1% was prepared by dissolving agar (Sigma-Aldrich, St. Louis, MO) into distilled deionized water. The solution was then magnetically stirred while a preservative of 1% potassium sorbate (Sigma-Aldrich, St. Louis, MO) and 1% cellulose particles (Sigma-Aldrich, St. Louis, MO) with size 20 μm were added to provide ultrasonic scattering. The solution was heated to 90 °C, degassed, poured in a 9 × 9 × 6 cm and a 9 × 9 × 4 cm containers for shear wave measurements (width × length × height) without tissue layer and with tissue layer respectively, and a 5 × 5 × 1.6 cm container for speed of sound and attenuation measurements (width × length × height) and cooled to room temperature. The tissue samples consisted of the skin, subcutaneous fat, and muscle. Fig. 1 shows pictures of the tissue samples. The tissue samples thickness were approximately 23.75 mm for Sample 1 and 23.25 mm for Sample 2. All experimental measurements were obtained in saline bath close to body temperature (33 °C to 35 °C).

Amador C., Aristizabal S., Greenleaf J.F, & Urban M.W. (2016). Phase Aberration and Attenuation Effects on Acoustic Radiation Force-Based Shear Wave Generation. IEEE transactions on ultrasonics, ferroelectrics, and frequency control, 63(2), 222-232.

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Cultivation of Coriolus hirsutus Fungus

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Coriolus hirsutus (MYA-828) was obtained from the American Type Culture Collection (ATCC; Manassas, VA). The additives include KCl and mannitol (BDH Inc.; Toronto, ON), glycerol (MP Biomedicals; Solon, OH), sucrose and CuSO₄ (ACP Chemical Inc.; Montreal, QC) as well as bovine serum albumin (BSA), dextran, sodium benzoate, sorbitol, potassium sorbate and the substrate syringaldazine (SG) (Sigma Chemical Co.; St. Louis, MO). The anhydrous ethanol was obtained from Commercial Alcohols Inc. (Branchville, QC). Other chemicals used for the preparation of reagents and buffers were purchased from Fisher Scientific (Fair Lawn, N.J.) and prepared in deionized water, using Milli Q plus (Millipore; Milford, MA).

Bou-Mitri C, & Kermasha S. (2018). Lyoprotection and stabilization of laccase extract from Coriolus hirsutus, using selected additives. AMB Express, 8, 152.

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Buffers for Immunoassay Development

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All buffers were prepared in Milli-Q water and stored in amber glass bottles at room temperature (RT, 22 ± 1 °C) unless stated otherwise. The pH values were adjusted using 6 M hydrochloric acid (Merck) or 5 M sodium hydroxide solution (J.T.Baker, Phillipsburg, NJ, USA).

Phosphate-buffered saline (PBS), pH 7.6: 10 mM sodium phosphate monobasic dihydrate (Sigma-Aldrich), 70 mM sodium phosphate dibasic dihydrate (Sigma-Aldrich), 145 mM sodium chloride (Sigma-Aldrich).

Washing buffer, pH 7.6: 0.75 mM potassium phosphate monobasic (Sigma-Aldrich), 6.25 mM potassium phosphate dibasic (Sigma-Aldrich), 0.025 mM potassium sorbate (Sigma-Aldrich), 0.05% Tween 20 (Serva, Heidelberg, Germany).

Assay buffer (Tris–EDTA), pH 7.6, storage at 4 °C: 125 mM tris(hydroxymethyl)-aminomethane (Tris, Merck), 187.5 mM sodium chloride, 13.375 mM ethylenediaminetetraacetic acid disodium salt dihydrate (Na₂EDTA·2H₂O, Sigma-Aldrich).

Citrate buffer, pH 4.0, storage at 4 °C: 220 mM sodium citrate monobasic (Sigma-Aldrich).

TMB stock solution in dry N,N-dimethylacetamide (DMA, Sigma-Aldrich), storage under argon at 4 °C: 8 mM tetrabutylammonium borohydride (Sigma-Aldrich), 40 mM 3,3’,5,5’-tetramethylbenzidine (TMB, Serva).

Ecke A., Westphalen T., Hornung J., Voetz M, & Schneider R.J. (2021). A rapid magnetic bead-based immunoassay for sensitive determination of diclofenac. Analytical and Bioanalytical Chemistry, 414(4), 1563-1573.

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Characterization of Maize-Derived Compounds

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The de Man, Rogosa and Sharpe (MRS) agar/broth, glycerol (Analytical grade), nutrient agar/broth, Vogel’s agar/broth, ethyl acetate (HPLC grade), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2-oxopropanoic acid (pyruvic acid), 2-hydroxypropane-1,2,3-tricarboxylic acid (citric acid), 2-hydroxybutanedioic acid (malic acid), 2-hydroxypropanoic acid (malonic acid), propanedioic acid (lactic acid), butanedioic acid (succinic acid), β-carotene, sodium benzoate, potassium sorbate and sodium acetate were mainly purchased from Sigma-Aldrich, St. Louis, MO, USA. Maize grains (FMC Variety C-7065) were collected from a grain market in Faisalabad, Pakistan.

Salman M., Tariq A., Ijaz A., Naheed S., Hashem A., Abd_Allah E.F., Soliman M.H, & Javed M.R. (2020). In Vitro Antimicrobial and Antioxidant Activities of Lactobacillus coryniformis BCH-4 Bioactive Compounds and Determination of their Bioprotective Effects on Nutritional Components of Maize (Zea mays L.). Molecules, 25(20), 4685.

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Calcium-Enriched Brine for Cottage Cheese

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The cover brine contained 160.0 mM calcium chloride (CaCl₂) (Brenntag, Durham, NC, USA), 12.0 mM potassium sorbate (Sigma-Aldrich, St. Louis, MO, USA), 40.4 mM anhydrous calcium hydroxide Ca(OH)₂ (Brenntag, Durham, NC, USA) and acetic acid in the form of vinegar (Fleischmann's Vinegar Company Inc., Cerritos, CA, USA) to adjust the pH to 5.20 ± 0.05 (29 (link)). The cover brine and CJM were mixed in a 50:50 (v:v) ratio diluting all constituents by 50%.

Rothwell M.A., Zhai Y., Pagán-Medina C.G, & Pérez-Díaz I.M. (2022). Growth of ɣ-Proteobacteria in Low Salt Cucumber Fermentation Is Prevented by Lactobacilli and the Cover Brine Ingredients. Microbiology Spectrum, 10(3), e01031-21.

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Antimicrobial Agents and Food Preservation

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Sodium dichloroisocyanurate, trichloroisocyanuric acid, 4-chloro-3,5-dimethylphenol, McLean Biochemical Technology Co., Ltd., Shanghai, China; terbium chloride(TbCl₃), Nycodenz, tea polyphenols (catechin content accounted for 41.31%, epicatechin content accounted for 19.72%, quercetin content accounted for 3.71%, and gallic acid content accounted for 4.75%), Macklin Biochemical Technology Co., Ltd., Shanghai, China; nisin, nitrite, potassium sorbate, D-isoascorbic acid, sodium diacetate, ԑ-polylysine hydrochloride, Sigma-Aldrich Trading Co., Ltd., Shanghai, China; tryptose Sulfite cycloserine Agar Base (TSC), thioglycollate medium (FTG), Hi-tech Industrial Park Haibo Biotechnology Co., Ltd., Shandong, China; whey protein fermentation powder, yinong biology science and technology Co., Ltd., Shanghai, China; Other reagents such as glutaraldehyde, silver nitrate standard solution and hydrogen peroxide solution are analytically pure in China.

Liang D., Liu S., Li M., Zhu Y., Zhao L., Sun L., Ma Y, & Zhao G. (2023). Effects of Different Bacteriostats on the Dynamic Germination of Clostridium perfringens Spores. Foods, 12(9), 1834.

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Characterization of Native Cassava Starch

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Native cassava starch (13.1% moisture) was food-grade (Bernesa S.A., Lomas de Zamora Buenos Aires, Argentina). The starch used was characterized by a degree of purity of 92–98% w/w. The amylose content was 19 % w/w, the amylopectin content was 81 % w/w, and the average molecular weight was 68 × 10⁶ Da for amylopectin and 0.8 × 10⁶ Da for amylose [18 (link)]. Analytical-grade glycerol (Biopack, Zárate, Buenos Aires, Argentina) and potassium sorbate (Sigma, St. Louis, MI, USA) were used. Deionized water was employed.

Otálora González C.M., Felix M., Bengoechea C., Flores S, & Gerschenson L.N. (2024). Development and Characterization of Edible Films Based on Cassava Starch Modified by Corona Treatment. Foods, 13(3), 468.

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Flor Velum Yeast Metabolism in Bee Pollen Wine

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To identify bee pollen addition influence on flor velum yeast metabolism during biological aging, oenological parameters, major volatile compounds, and organic acids were assessed in all samples. Flor velum at a concentration of 7.4 × 10⁴ ± 114 CFU/mL was inoculated in 200 mL of bee pollen supplemented wine inside Greiner Bio-One flasks (Kremsmünster, Germany) stored in the same conditions as the test tubes noted in Section 2.3. Under the same conditions, 200 mg/L of potassium sorbate (Sigma–Aldrich, Saint Louis, MO, United States) were added to another 200 mL flask of wine to control the ethanol amount evaporated during the biological aging process. All the experiments were conducted in triplicate. Once the experiment was finished, wines were filtered using 0.2 µm membrane filters (Darmstad, Germany) and bottled using an inert atmosphere (N₂) to avoid changes until their characterization and sensory analysis.

Sancho-Galán P., Amores-Arrocha A., Jiménez-Cantizano A, & Palacios V. (2019). Use of Multiflora Bee Pollen as a Flor Velum Yeast Growth Activator in Biological Aging Wines. Molecules, 24(9), 1763.

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