The tissue samples were collected during the THA revision surgery from a patient who suffered from a suddenly fractured long straight modular neck of a commonly used bi-modular femoral stem (Profemur® Z, Wright Medical Technology, now MicroPort Orthopedics, Arlington, TN, USA) with an oval Morse taper as the neck-stem junction. Both neck and stem were made of Ti alloy containing Ti, Al and Vanadium (V): 90Ti, 6Al and 4V (wt%). The study was approved by the author’s (SKF) institutional review board (IRB) and the patient gave informed consent to participate. Samples were sent for cultures, histologic and high-resolution multimodal examination. The biopsy tissue was stained to delimit the surgical biopsy borders with the Davidson Marking System® (DMS) in blue colour (#3408-5), from Bradley Products, Inc. (Minneapolis, MN, USA). The tissue was fixed in formalin (10% neutral buffered formalin) and placed in paraffin blocks with the standard process using an ExcelsiorTM AS Tissue Processor (Thermo Fisher Scientific, Waltham, MA, USA) and Tissue Embedding System TES99 (MEDITE Cancer Diagnostics, Chicago, IL, USA) (Figure 2 ).
Excelsior as tissue processor
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom
The Excelsior AS Tissue Processor is a laboratory instrument designed for the automated processing of tissue samples. It is used to prepare tissue specimens for histological examination and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Histostar embedding workstation, by Thermo Fisher Scientific (6 mentions)
Neutral buffered formalin, by Merck Group (4 mentions)
Embedding workstation, by Thermo Fisher Scientific (3 mentions)
Waterfall hm325 microtome, by Thermo Fisher Scientific (3 mentions)
Axio imager a2, by Zeiss (3 mentions)
Nuclear fast red stain, by Merck Group (3 mentions)
Nuclear fast red, by Merck Group (3 mentions)
Zen software, by Zeiss (3 mentions)
Superfrost plus adhesion slides, by Thermo Fisher Scientific (2 mentions)
Micron hm325 rotary microtome, by Thermo Fisher Scientific (2 mentions)
Pro 03, by Matsunami (2 mentions)
Neutral buffered formalin, by Thermo Fisher Scientific (2 mentions)
Hm 355s automatic microtome, by Thermo Fisher Scientific (2 mentions)
Axioskop 2 plus, by Zeiss (2 mentions)
Hematoxylin and eosin, by Diapath (1 mentions)
Rnascope multiplex fluorescent v2 assay, by Advanced Cell Diagnostics (1 mentions)
Paraplast plus, by Leica (1 mentions)
Hematoxylin and eosin, by Thermo Fisher Scientific (1 mentions)
Hm355s microtome, by Thermo Fisher Scientific (1 mentions)
Gemini stainer, by Thermo Fisher Scientific (1 mentions)
34 protocols using excelsior as tissue processor
1
Tissue Analysis of Fractured Modular Neck
2
Paraffin-embedding of Octopus embryos
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Live O. vulgaris embryos were obtained from the lab of E. Almansa (IEO, Tenerife), transferred to the lab of Developmental neurobiology and kept in a closed standalone system (Deryckere et al., 2020) (link).
Embryos were observed, staged and sampled daily, followed by overnight fixation in 4 % paraformaldehyde (PFA) in phosphate buffered saline (PBS). After a wash in PBS, embryos were manually dechorionated with forceps and transferred to embedding cassettes (Tissue-Tek Biopsy 6-Chamber Cassette, Sakura). For paraffin processing, the cassettes were immersed in 0.9 % NaCl overnight before progressive dehydration and paraffin-embedding using an Excelsior TM AS Tissue Processor and HistoStar TM Embedding Workstation (Thermo Scientific). 6 μm-thick transversal sections were made for subsequent immunohistochemistry or in situ hybridization.
Embryos were observed, staged and sampled daily, followed by overnight fixation in 4 % paraformaldehyde (PFA) in phosphate buffered saline (PBS). After a wash in PBS, embryos were manually dechorionated with forceps and transferred to embedding cassettes (Tissue-Tek Biopsy 6-Chamber Cassette, Sakura). For paraffin processing, the cassettes were immersed in 0.9 % NaCl overnight before progressive dehydration and paraffin-embedding using an Excelsior TM AS Tissue Processor and HistoStar TM Embedding Workstation (Thermo Scientific). 6 μm-thick transversal sections were made for subsequent immunohistochemistry or in situ hybridization.
3
Hematoxylin-Eosin Staining of Spleen and Bone Marrow
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Spleen tissue and sternum were collected and fixed in 10% neutral buffered formalin (Sigma) for 24 hours and then processed for paraffin embedding (Thermo Scientific ExcelsiorTM AS Tissue Processor and HistoStarTM Embedding Workstation). Sections of 5 μm were mounted on SuperfrostTM Plus Adhesion slides (Thermo Scientific) and stained with hematoxylin and eosin (Diapath). The degree of splenic or bone marrow colonization by leukemic cells was determined by staining with human HLA-A (Abcam) with a digital image analysis algorithm created on the ImageJ software platform.
4
Developmental Expression of TRα and TRβ in Tadpole Liver
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Livers were collected from wild type tadpoles at NF stage 54, 58, 61, 66, and TRdKO tadpoles at NF stage 54, stage 58, and stage 61. The livers were fixed with NBF overnight and transferred into 70% ethanol, processed with a tissue processor (Excelsior AS Tissue Processor; Thermo Fisher Scientific, Waltham, MA), and sectioned at 5 μm. RNAscope Multiplex Fluorescent V2 Assay (Advanced Cell Diagnostics (ACD), Newark, CA) was carried out to detect TRα and TRβ mRNAs. Target probes and the control β-actin probe were designed by ACD (Table S2 ).
5
Histological Analysis of Lung Tissues
6
Tissue Harvesting and Processing Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animal were euthanised with 10% CO2 for five minutes at each time point. They were confirmed dead when they stopped breathing, did not have a heartbeat, and did not respond to a firm toe pinch. The animals were shaved, a dorsal midline incision was made using a scalpel, and the adjacent fascia was released via gentle dissection to find the explanted tissue. The dissected tissues were transferred into a bowl filled with 1xPBS before being fixed in the 10% neutral buffered formalin.
The skins with implanted scaffolds, kidneys, and livers of the euthanised animals were fixed in 10% (v/v) neutral buffered formalin overnight and processed and processed in an automated Excelsior™ AS Tissue Processor (Thermo Fisher Scientific, Waltham, MA, USA) for 16 h. Tissues were embedded in paraffin (Paraplast® Plus, Leica Biosystems, Richmond, IL, USA) and cut into 3 to 5 μm sections with a rotary microtome Histo-Tek SRM II (Sakura Finetek, Torrance, CA, USA). The sections were transferred into a water bath at 37 °C and then mounted on frosted-end slides (HmbG GmbH, Hamburg, Germany) for basic staining.
The skins with implanted scaffolds, kidneys, and livers of the euthanised animals were fixed in 10% (v/v) neutral buffered formalin overnight and processed and processed in an automated Excelsior™ AS Tissue Processor (Thermo Fisher Scientific, Waltham, MA, USA) for 16 h. Tissues were embedded in paraffin (Paraplast® Plus, Leica Biosystems, Richmond, IL, USA) and cut into 3 to 5 μm sections with a rotary microtome Histo-Tek SRM II (Sakura Finetek, Torrance, CA, USA). The sections were transferred into a water bath at 37 °C and then mounted on frosted-end slides (HmbG GmbH, Hamburg, Germany) for basic staining.
7
Histological Analysis of Hybrid Gonads
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
38 hatched hybrid individuals and 11 Guinea fowl controls were raised to maturity and in every two weeks between the 16th and 30th week of growth 4–5 hybrids and one or two control animals were sacrificed for histological analysis. The gonads were removed, imaged, and then fixed in 8% paraformaldehyde solution for 1–2 days (Excelsior AS Tissue Processor, No.: A82300001, Thermofisher).
Gonads were washed under running water, dehydrated in increasing concentrations of ethanol, then transferred to paraffin at 75 °C and placed in an embedding cassette (Paraffin Dispenser WD-4C, No.: 205510, Kunz Instrumentz) for the preparation of histological sections. Paraffin was congealed on a cooling plate (Cooling Plate CP-4D, No.: 205600, Kunz Instrumentz) and the paraffin blocks were cut into 3–4 µm-thick sections. After the hardening of the sections, hematoxylin-eosin staining was performed (Shandon Varistain 24–4 Slide Stainer, No.: 8358-30-1025) and the sections were covered for microscopic examination (Zeiss Axioskop 2 plus) at x100 magnification.
Gonads were washed under running water, dehydrated in increasing concentrations of ethanol, then transferred to paraffin at 75 °C and placed in an embedding cassette (Paraffin Dispenser WD-4C, No.: 205510, Kunz Instrumentz) for the preparation of histological sections. Paraffin was congealed on a cooling plate (Cooling Plate CP-4D, No.: 205600, Kunz Instrumentz) and the paraffin blocks were cut into 3–4 µm-thick sections. After the hardening of the sections, hematoxylin-eosin staining was performed (Shandon Varistain 24–4 Slide Stainer, No.: 8358-30-1025) and the sections were covered for microscopic examination (Zeiss Axioskop 2 plus) at x100 magnification.
8
Alcian Blue Staining of Cartilage Organoids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For Alcian Blue detection of cartilage, organoids were fixed in 4% PFA as described above and processed for routine paraffin embedding using the Excelsior AS Tissue Processor (rapid biopsy setting; Thermo Fisher Scientific). Samples were embedded in wax and 5μm sections cut using a Waterfall HM325 microtome (Thermo Fisher Scientific). Sections were dewaxed, hydrated through graded alcohols to running water, then covered with Alcian Blue Solution (1% Alcian blue in 3% acetic acid, pH 2.5). After 10 minutes, sections were washed in tap water for 2 minutes and counterstained for 7 minutes in Nuclear Fast Red stain (0.1% Nuclear Fast Red [Sigma Aldrich, St Louise, MO] and 5% ammonium potassium sulfate in water). Following staining, sections were dehydrated in graded alcohols, cleared in Safsolvent (Bacto Laboratories, NSW, Australia), and coverslipped. Images were acquired on a Zeiss Axio Imager A2 with Zeiss Zen software (Zeiss Microscopy, Thornwood, NY).
9
Histological Analysis of Lung Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dissected lung tissues were fixed in 10% neutral buffered formalin (Sigma-Aldrich, St. Louis, USA). With the use of an automated tissue processor (Excelsior AS Tissue Processor, Thermo Scientific, Waltham, USA), airway samples underwent dehydration process in ascending grades of ethanol and two washes of xylene. Tissue samples were embedded in paraffin for analyses (HistoStar Embedding Workstation, Thermo Scientific, Waltham, USA). Tissue sections cut at 4-micron thickness were prepared using a microtome sectioner (Micron HM325 Rotary Microtome, Thermo Scientific, Waltham, USA) and heated water bath. Following mounting on coated slides (PRO-03; Matsunami, Osaka, Japan), sections were deparaffinised in xylene and rehydrated in graded ethanol prior to staining. Haematoxylin and Eosin (H&E) staining was used to assess the structural integrity, inflammation and the absence or presence of additional pathologies.
10
Histological Analysis of Spleen Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Spleen tissue was fixed overnight in 10% neutral buffered formalin (Sigma) and then transferred to 50% ethanol. The samples were processed for paraffin embedding (Thermo Scientific Excelsior AS Tissue Processor and HistoStar Embedding Workstation). Sections of 4 μm were mounted on Superfrost Plus Adhesion slides (Thermo Fisher Scientific) and stained with hematoxylin and eosin (Diapath). For CD3 and Cre stainings, heat-induced antigen retrieval was performed, and the sections were subsequently blocked with normal goat (Cre) or horse (CD3) serum. The sections were incubated overnight with the primary antibody at 4°C and incubated with the secondary antibody (conjugated with biotin-free HRP) for 30-45 min at room temperature. Visualisation was performed using a peroxidase substrate, and hematoxylin counterstaining was carried out.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!