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Tlc sg

Manufactured by Merck Group
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TLC-SG is a thin-layer chromatography (TLC) product from Merck Group, designed for separation and analysis of chemical compounds. It serves as a platform for performing TLC, a fundamental technique in analytical chemistry. The product provides a solid support for the separation process, facilitating the identification and purification of various substances.

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Lab products found in correlation

Wallac wizard 1470 020 gamma counter, by PerkinElmer (4 mentions) Wallace wizard 1470 020 gamma counter, by PerkinElmer (1 mentions)

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TLC-SG F254 (2 citations) TLC-SG (Al) strips (2 citations)

6 protocols using tlc sg

1

Radiochemical Purity Determination

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Radiochemical purity was determined by thin layer chromatography (TLC-SG, Merck) using acetone as the mobile phase to quantify 99mTcO4-. Strips radioactivity was determined by a gamma counter (Wallac Wizard 1470–020 Gamma Counter, PerkinElmer Inc.). 99mTcO2 was removed from the preparation using a 0.45 μm syringe filter [32 (link)].
de Oliveira-Mendes B.B., Miranda S.E., Sales-Medina D.F., Magalhães B.D., Kalapothakis Y., de Souza R.P., Cardoso V.N., de Barros A.L., Guerra-Duarte C., Kalapothakis E, & Horta C.C. (2019). Inhibition of Tityus serrulatus venom hyaluronidase affects venom biodistribution. PLoS Neglected Tropical Diseases, 13(4), e0007048.
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2

Radiolabeling of NE-LAP with Technetium-99m

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Radiolabeling of NE-LAP was carried out in a sealed vial containing 1.0 mL NE-LAP and 100 μL SnCl2·H2O solution in 0.25 mol L −1HCl (1.0 mg/mL). The pH was adjusted to 7.4 using NaOH (1 mol L −1), and vacuum was performed to the vial. An aliquot of 0.1 mL of Na99mTcO4 (3.7 MBq) was added to the vial and maintained at room temperature for 15 min.
Radiolabeling yield was determined by thin-layer chromatography (TLC-SG, Merck, Darmstadt, Germany) using acetone as the mobile phase to quantify 99mTcO4− . Radioactivity was determined using a gamma counter (Wallac Wizard 1470–020 Gamma Counter, PerkinElmer Inc., Waltham, Massachusetts, USA). 99mTcO2 was removed from the preparation using a 0.45 μm syringe filter [26 (link)].
Mendes Miranda S.E., de Alcântara Lemos J., Fernandes R.S., Silva J.D., Ottoni F.M., Townsend D.M., Rubello D., Alves R.J., Cassali G.D., Ferreira L.A, & de Barros A.L. (2020). Enhanced antitumor efficacy of lapachol-loaded nanoemulsion in breast cancer tumor model. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 133, 110936.
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3

Radiochemical Purity Analysis of 99mTc

Cited 1 time
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Radiochemical purity analyses were performed by thin layer chromatography on silica gel (TLC-SG; Merck, Darmstadt, Germany) using acetone as mobile phase to quantify free 99mTcO4−. The radioactivity was determined by a gamma counter (Wallac Wizard 1470–020 Gamma Counter; PerkinElmer Inc., Waltham, Massachusetts, USA). The solution was purified from 99mTcO2 using a 0.22 μm syringe filter, as previously described [23 (link),24 ].
Monteiro L.O., Fernandes R.S., Oda C.M., Lopes S.C., Townsend D.M., Cardoso V.N., Oliveira M.C., Leite E.A., Rubello D, & de Barros A.L. (2017). Paclitaxel-loaded folate-coated long circulating and pH-sensitive liposomes as a potential drug delivery system: A biodistribution study. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 97, 489-495.
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4

Radiolabeling of Paclitaxel with Technetium-99m

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A 0.1 ml aliquot of the PTX dispersion was added to 300 µg of SnCl2•2H2O in an acid solution (HCl 0.25 M) and adjusted to pH 7.4 using NaOH solution (0.1 M). A 0.1 ml aliquot of Na99mTcO4 (37 MBq) was added and the solution was kept at room temperature for 15 min.
Radiochemical purity was determined by Thin Layer Chromatography on silica gel (TLC-SG; Merck, Darmstadt, Germany) using acetone as mobile phase to quantify free 99mTcO4−. The radioactivity was determined using a Wallace Wizard 1470–020 Gamma Counter (PerkinElmer Inc., Waltham, Massachusetts, USA). The solution was purified from 99mTcO2 using a 0.22 µm syringe filter, as previously described by Fernandes et al. [12 (link)].
Monteiro L.O., Fernandes R.S., Castro L.C., Cardoso V.N., Oliveira M.C., Townsend D.M., Ferretti A., Rubello D., Leite E.A, & de Barros A.L. (2017). Technetium-99 m radiolabeled paclitaxel as an imaging probe for breast cancer in vivo. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 89, 146-151.
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5

Radiochemical Purity Analysis of [99mTc]O4−

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Radiochemical purity analyses were performed by thin-layer chromatography (TLC) on silica gel (TLC-SG; Merck, Darmstadt, Germany) to determine the amount of free [99mTc]O4−, and both solutions were purified from [99mTc]O2 using a 0.22-μm syringe filter. More details are given in ESM.
Fernandes R.S., Silva J.O., Mussi S.V., Lopes S.C., Leite E.A., Cassali G.D., Cardoso V.N., Townsend D.M., Colletti P.M., Ferreira L.A., Rubello D, & de Barros A.L. (2018). Nanostructured Lipid Carrier Co-loaded with Doxorubicin and Docosahexaenoic Acid as a Theranostic Agent: Evaluation of Biodistribution and Antitumor Activity in Experimental Model. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 20(3), 437-447.
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6

Radiolabeling of NE-LAP with Technetium-99m

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Radiolabeling of NE-LAP was carried out in a sealed vial containing 1.0 mL NE-LAP and 100 μL SnCl2·H2O solution in 0.25 mol L −1HCl (1.0 mg/mL). The pH was adjusted to 7.4 using NaOH (1 mol L −1), and vacuum was performed to the vial. An aliquot of 0.1 mL of Na99mTcO4 (3.7 MBq) was added to the vial and maintained at room temperature for 15 min.
Radiolabeling yield was determined by thin-layer chromatography (TLC-SG, Merck, Darmstadt, Germany) using acetone as the mobile phase to quantify 99mTcO4− . Radioactivity was determined using a gamma counter (Wallac Wizard 1470–020 Gamma Counter, PerkinElmer Inc., Waltham, Massachusetts, USA). 99mTcO2 was removed from the preparation using a 0.45 μm syringe filter [26 (link)].
Mendes Miranda S.E., de Alcântara Lemos J., Fernandes R.S., Silva J.D., Ottoni F.M., Townsend D.M., Rubello D., Alves R.J., Cassali G.D., Ferreira L.A, & de Barros A.L. (2020). Enhanced antitumor efficacy of lapachol-loaded nanoemulsion in breast cancer tumor model. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 133, 110936.
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