Wild type cd1 females

Manufactured by Charles River Laboratories

Wild-type CD1 females are laboratory mice used in scientific research. They are a common strain of mice that have not been genetically modified. These mice are used as control animals in various experiments to establish baseline data.

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Lab products found in correlation

C57bl 6j, by Jackson ImmunoResearch (1 mentions) Cd 1 igs, by Charles River Laboratories (1 mentions) M2 media, by Merck Group (1 mentions) Dat tta, by Jackson ImmunoResearch (1 mentions) Rosa26ai9, by Charles River Laboratories (1 mentions) Rosa26ai9, by Jackson ImmunoResearch (1 mentions)

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CD1 females (37 citations)

4 protocols using wild type cd1 females

Inducible Fate Mapping of CXCR4+ Cells

Cited 3 times

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All mouse experiments were carried out with strict observance of protocols and guidelines approved by the University of Bonn Animal Care and Use Committee, Federal Government of Germany and European Union legislation. The protocols were approved by the Landesamt für Natur, Umwelt und Verbraucherschutz Nordrhein-Westfalen (Permit Number: 84-02.02.2016.A238). The mice were housed under controlled light (12 h light/dark cycle at an ambient temperature of 22°C). Water and mice chow were available ad libitum.
Cxcr4^{CreER(T2)-IRES-eGFP}(Cxcr4^CreER) mice were provided by Ralf Stumm (Werner et al., 2020 (link)). Rosa26^Ai9 (stock #007909; Madisen et al., 2010 (link)), Dat^tTA (stock #027178; Chen et al., 2015 (link)), and Ai82D (lgs7^TITLGFP; stock #023532; Madisen et al., 2015 (link)) mice were purchased from The Jackson Laboratory. For inducible fate-mapping studies, Cxcr4^CreER/+; Rosa26^Ai9/Ai9 males were bred with CD1 wild-type females (Charles River) to generate Cxcr4^CreER/+; Rosa26^Ai9/+ progeny. For intersectional fate mapping, Cxcr4^CreER/+; Dat^tTA/+; lgs7^{TITLGFP/TITLGFP} males were bred with CD1 wild-type females (Charles River) to generate Cxcr4^CreER/+; Dat^tTA/+; lgs7^TITLGFP progeny. Mice of either sex were used for experiments.

Petese A., Fries F.L., Broske B., Stumm R, & Blaess S. (2022). Lineage Analysis of Cxcr4-Expressing Cells in the Developing Midbrain Suggests That Progressive Competence Restriction in Dopaminergic Progenitor Cells Contributes to the Establishment of Dopaminergic Neuronal Diversity. eNeuro, 9(4), ENEURO.0052-22.2022.

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Heterozygous Esr1-Cre Mice Generation

Cited 1 time

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Male aggressors were generated by breeding homozygous Esr1 (ERa)-Cre males on a C57BL/6J background (The Jackson Laboratory, Bar Harbor, Maine) with wild type CD-1 females (Charles River Laboratories) ^{39 (link)}. This breeding strategy ensured that all F1 inherited heterozygous Esr1-Cre alleles that was confirmed by random genotyping of the offspring.

Deonaraine K., Wang Q., Cheng H., Chan K.L., Lin H.Y., Liu K., Parise L.F., Cathomas F., Leclair K.B., Flanigan M.E., Li L., Aleyasin H., Guevara C., Hao K., Zhang B., Russo S.J, & Wang J. (2020). Sex-specific peripheral and central responses to stress-induced depression and treatment in a mouse model. Journal of neuroscience research, 98(12), 2541-2553.

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Generation and Staging of Mutant Mice

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Two mutant mouse lines were designed and produced using CRISPR/Cas9 in collaboration with the Vanderbilt Genome Editing Resource in Nashville, TN, USA. The Vanderbilt Institutional Animal Care and Use Committee approved all experimental procedures in accordance with the ethics guidelines at Vanderbilt. Mice are socially housed within Vanderbilt’s animal facility with a 12-hour light-dark cycle. Both mouse lines are maintained in an outbred Crl:CD1(ICR), or CD-1® IGS background (Charles River, model #022) by breeding heterozygous males with wild-type CD1 females obtained from Charles River.
For stage-specific embryo collections, timed matings were performed by placing 8-week-old heterozygous male mice that had been single-housed for 1-2 weeks with 1 to 2 group-housed heterozygous females that were also 6-8 weeks old. Female mice with vaginal plugs were defined as being 0.5 days post coitum (or embryonic day 0.5 – E0.5). At desired developmental time points, embryonic tissues were micro-dissected in cold phosphate buffered saline (PBS) and staged according to Theiler staging criteria.
Mouse genotypes were determined using DNA extracted from adult tail biopsy or embryonic yolk sac lysates. Wild-type embryos from the same litters were used as controls for CR-null embryos.

Trinh L.T., Osipovich A.B., Sampson L., Wong J., Wright C.V, & Magnuson M.A. (2022). Differential regulation of alternate promoter regions in Sox17 during endodermal and vascular endothelial development. iScience, 25(9), 104905.

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Timed Embryo Isolation from Transgenic Mice

Cited 1 time

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Embryos were obtained through natural matings of heterozygous transgenic CAG-TAG1 males (Trichas et al., 2008 (link)) with wild-type CD1 females (Charles River). Noon of the day when the vaginal plug was found was considered 0.5 days post coitum (dpc). Embryos were isolated from the oviduct at 2.5 dpc in M2 media (Sigma) and then held in KSOM (Millipore catalogue number MR-020P-5F) before setting up for culture.

Watanabe T., Biggins J.S., Tannan N.B, & Srinivas S. (2014). Limited predictive value of blastomere angle of division in trophectoderm and inner cell mass specification. Development (Cambridge, England), 141(11), 2279-2288.

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