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Modular incubator chamber

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The Modular Incubator Chamber is a versatile and configurable laboratory equipment designed to provide a controlled environment for cell and tissue culture applications. It maintains precise temperature, humidity, and gas composition settings to support optimal growth and development of biological samples.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (7 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (6 mentions) Polyjet, by SignaGen (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Albumax 2, by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (3 mentions) Tnf α, by R&D Systems (3 mentions) Fugene 6, by Promega (3 mentions) Glucose free dmem, by Thermo Fisher Scientific (3 mentions) Rpmi 1640 medium, by Corning (3 mentions) Cocl2, by Merck Group (3 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions) Hek293ft, by Thermo Fisher Scientific (2 mentions) Mcf 7, by American Type Culture Collection (2 mentions) Bevacizumab, by Roche (2 mentions) Mda mb 468, by American Type Culture Collection (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Glucose free medium, by Cell Biologics (2 mentions) Hpaecs, by Embrient Inc (2 mentions)

Spelling variants of this product

Modular Incubator Chamber (MIC-101; (11 citations) Hypoxia modular incubator chamber (8 citations) Modular chamber (7 citations) Incubator chamber (7 citations) Airtight modular incubator chamber (7 citations) Sealed modular incubator chamber (5 citations) Anaerobic modular incubator chambers (2 citations)

127 protocols using modular incubator chamber

1

Hypoxic Exposure in Pregnant Mice

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Timed pregnant mice (MATcKO, ODD-Luc) were placed in modular incubator chambers (Billups-Rothenberg Inc.) on E11.5, 13.5 or 15.5. The chamber was flushed with gas from a tank containing 8% O2/ 92% nitrogen (Airgas) and then clamped. The mice were maintained in this chamber for 4 hours with free access to food and water and tolerated this procedure well. For control experiments, pregnant dams were placed in the chamber and flushed with gas from a tank containing 21 % O2 / 79% nitrogen (room air), for 4 hours with free access to food and water.
Kenchegowda D., Natale B., Lemus M., Natale D.R, & Fisher S.A. (2016). Inactivation of maternal Hif-1α at mid-pregnancy causes placental defects and deficits in oxygen delivery to the fetal organs under hypoxic stress. Developmental biology, 422(2), 171-185.
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2

Rearing Lepidopteran Larvae in Controlled Environment

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Larvae (n=24 per diet) were reared for the entirety of larval growth in 48-well plates that were stored inside of modular incubator chambers (Billups-Rothenberg, del Mar, CA, USA), containing a small volume of 96% K2SO4 to maintain high humidity. Chambers were kept at a constant 34°C in a dark environmental chamber. Each day, larvae were removed from the environmental chamber and an additional day-specific volume of artificial diet was added to each well as follows: days 1 and 2, 10 µl; day 3, 20 µl; day 4, 30 µl; day 5, 40 µl; and day 6, 50 µl. Each larva was provisioned with a total of 160 µl of artificial diet. Larvae were kept in these conditions until they completely finished consuming their provisions, at which time they were removed from well plates and moved to pupation plates or declared dead and removed from the study. Death was indicated by repeated days of immobility, lack of growth, and blackened appearance.
Helm B.R., Slater G.P., Rajamohan A., Yocum G.D., Greenlee K.J, & Bowsher J.H. (2017). The geometric framework for nutrition reveals interactions between protein and carbohydrate during larval growth in honey bees. Biology Open, 6(6), 872-880.
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3

Hypoxia Regulation of A549 Cells

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The human lung adeno-carcinoma cell line A549 was grown in DMEM supplemented with penicillin, streptomycin and 10% heat-inactivated FBS at 37°C in a humidified atmosphere containing 5% CO2. Hypoxia was defined as 1% oxygen, which was achieved by culturing cells in modular incubator chambers (Billups-Rothenberg, Inc., Del Mar, CA, USA), which were flushed with gas mixtures (95% nitrogen/5% carbon dioxide) and sealed to maintain hypoxia.
Cells were seeded into 35-mm dishes (Iwaki, Chiba, Japan) at 2×105 cells/dish with 1.5 ml medium containing LW6 for 12 h. Cells were incubated under normoxia or hypoxia for 36 h and were then assessed for the expression of HIF-1α and the ratio of apoptotic cells. To analyze active caspase-3, the cells treated with LW6 for 12 h were exposed to hypoxia or normoxia for 48 h and the cells were then analyzed.
SATO M., HIROSE K., KASHIWAKURA I., AOKI M., KAWAGUCHI H., HATAYAMA Y., AKIMOTO H., NARITA Y, & TAKAI Y. (2015). LW6, a hypoxia-inducible factor 1 inhibitor, selectively induces apoptosis in hypoxic cells through depolarization of mitochondria in A549 human lung cancer cells. Molecular Medicine Reports, 12(3), 3462-3468.
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4

Hypoxic and Normoxic Cell Incubation

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Hypoxic cells were incubated under 1% O2 and 10% CO2 in modular incubator chambers (Billups-Rothenberg). Normoxic cells were incubated at 10% CO2 and 20% O2.
Mariani C.J., Vasanthakumar A., Madzo J., Yesilkanal A., Bhagat T., Yu Y., Bhattacharyya S., Wenger R.H., Cohn S.L., Nanduri J., Verma A., Prabhakar N.R, & Godley L.A. (2014). TET1-Mediated Hydroxymethylation Facilitates Hypoxic Gene Induction in Neuroblastoma. Cell reports, 7(5), 1343-1352.
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5

Culturing Plasmodium falciparum Strains

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P. falciparum W2, 3D7, and Dd2_Pold cultures were maintained, synchronized, and lysed as previously described (Straimer et al., 2017). Parasite cultures were grown in human erythrocytes (no blood type discrimination) purchased from the Stanford Blood Center (approved by Stanford University) or the Interstate Blood Bank (for Columbia University). at 3% hematocrit in RPMI-1640 media, supplemented with 25 mM HEPES, 50 mg L-hypoxanthine, 2mM L-glutamine, 0.225% sodium bicarbonate, 0.5% (wt/vol) AlbuMAXII (Invitrogen) and 10 μg/mL gentamycin. Cultures were maintained at 37°C in modular incubator chambers (Billups- Rothenberg) at 5% O2, 5% CO2 and 90% N2.
Bennett J.M., Narwal S.K., Kabeche S., Abegg D., Hackett F., Yeo T., Li V.L., Muir R.K., Faucher F.F., Lovell S., Blackman M.J., Adibekian A., Yeh E., Fidock D.A, & Bogyo M. (2024). Mixed Alkyl/Aryl Phosphonates Identify Metabolic Serine Hydrolases as Antimalarial Targets. bioRxiv.
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6

Islet Culture under Oxygen Levels

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Islets were cultured using a 24-well plate at a concentration of 250 IEQ/well in a cell culture insert (PICM01250, EMD Millipore, Billerica, MA, USA). IEQ is the standard unit used to express the estimate of islet number [24 (link)]. Cells were maintained in 800 μL of RPMI1640 medium (Life Technologies, Carlsbad, CA, USA) containing 5 mmol/L glucose and 10% heat inactivated fetal bovine serum (FBS, Atlanta Biologicals, Lawrenceville, GA, USA) at 37°C under seven different oxygen concentrations (1, 10, 21, 35, 50, 75, and 95% oxygen plus 5% CO2 and N2) in Modular Incubator Chambers (Billups-Rothenberg, San Diego, CA, USA). Oxygen toxicity experiments were performed using three day cultures; islet survival assessment was performed using seven day cultures.
Komatsu H., Cook C., Wang C.H., Medrano L., Lin H., Kandeel F., Tai Y.C, & Mullen Y. (2017). Oxygen environment and islet size are the primary limiting factors of isolated pancreatic islet survival. PLoS ONE, 12(8), e0183780.
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7

Neonatal Rat Cardiac Myocyte Isolation and Transfection

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Neonatal rat cardiac ventricular myocytes were isolated from 1–3 day old Sprague Dawley rats as described21 (link) and maintained in 10% fetal bovine serum containing culture media after isolation. For RNA interference ON-TARGETplus SMARTpool reagent against rat TGFbetaR1 was purchased from Dharmacon. ON-TARGETplus non-targeting pool was used as control. 24 hours after plating, cells were transfected with 25 nM siRNA using DharmaFECT 1 reagent following the manufacturer’s protocol in OptiMEM media (Gibco). 24 hours prior to cytokine stimulation cells were serum-starved. TNFα (50ng/ml), IL-1β (10ng/ml) (both R&D Systems) or TGFβ1 (5ng/ml, Sigma) were used for cytokine stimulation. For hypoxia studies, cells were exposed to a nitrogen atmosphere containing 5% CO2 and 1% O2 in modular incubator chambers (Billups-Rothenberg). PC3 cells transfected with hypoxia responsive elements (HRE) driving expression of GFP22 (link) and transcript expression of Slc2a1 (GLUT1) served to confirm effective hypoxia. RNA was harvested 24 hours after cytokine stimulation and/or exposure to hypoxia.
Rainer P.P., Hao S., Vanhoutte D., Lee D.I., Koitabashi N., Molkentin J.D, & Kass D.A. (2014). Cardiomyocyte-Specific TGFβ Suppression Blocks Neutrophil Infiltration, Augments Multiple Cytoprotective Cascades, and Reduces Early Mortality after Myocardial Infarction. Circulation research, 114(8), 1246-1257.
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8

Hypoxic culture of BMDM cells

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BMDM cells were seeded at a density of 2x105/ml. After an overnight incubation, the cells were washed twice in PBS and 1 ml culture medium without glucose (Life Technologies, Grand Island, NY) was added to each well. The plates were placed inside modular incubator chambers (Billups-Rothenberg, Del Mar, CA) with anaerobic colorimetric indicator strips that detect a 0.2% oxygen threshold (Becton Dickinson). The chamber was flushed with a gas mixture of 95% N2 and 5% CO2 for 20 min at room temperature at 6 L/min. After flushing, the chambers were sealed and maintained at 37°C for 18 hours.
Zhao J., Mou Y., Bernstock J.D., Klimanis D., Wang S., Spatz M., Maric D., Johnson K., Klinman D.M., Li X., Li X, & Hallenbeck J.M. (2015). Synthetic Oligodeoxynucleotides Containing Multiple Telemeric TTAGGG Motifs Suppress Inflammasome Activity in Macrophages Subjected to Oxygen and Glucose Deprivation and Reduce Ischemic Brain Injury in Stroke-Prone Spontaneously Hypertensive Rats. PLoS ONE, 10(10), e0140772.
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9

Hypoxic and Normoxic Cell Incubation

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Hypoxic cells were incubated under 1% O2 and 10% CO2 in modular incubator chambers (Billups-Rothenberg). Normoxic cells were incubated at 10% CO2 and 20% O2.
Mariani C.J., Vasanthakumar A., Madzo J., Yesilkanal A., Bhagat T., Yu Y., Bhattacharyya S., Wenger R.H., Cohn S.L., Nanduri J., Verma A., Prabhakar N.R, & Godley L.A. (2014). TET1-Mediated Hydroxymethylation Facilitates Hypoxic Gene Induction in Neuroblastoma. Cell reports, 7(5), 1343-1352.
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10

Hypoxic Cell Culture Conditions

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Hypoxic conditions were achieved by culturing cells in modular incubator chambers (Billups-Rothenberg Inc., Del Mar, CA, USA). The chambers, in which cell culture dishes and distilled water were housed, were flushed with mixed gas (95% nitrogen/5% carbon dioxide) to achieve the target oxygen pressure monitored using a JKO-02 Ver. III monitor (JIKCO, Tokyo, Japan), then sealed and incubated at 37°C.
Wada Y., Hirose K., Harada T., Sato M., Watanabe T., Anbai A., Hashimoto M, & Takai Y. (2018). Impact of oxygen status on 10B-BPA uptake into human glioblastoma cells, referring to significance in boron neutron capture therapy. Journal of Radiation Research, 59(2), 122-128.
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