Serotonin

Manufactured by Merck Group

Sourced in United States, Germany, France, Spain, Macao, Japan

Serotonin is a laboratory reagent used for analytical and research purposes. It is a monoamine neurotransmitter that plays a role in various physiological processes. The core function of serotonin is to facilitate chemical signaling in the body and brain.

Automatically generated - may contain errors

Lab products found in correlation

159 protocols using serotonin

Serotonin's Impact on Fungal Growth

Check if the same lab product or an alternative is used in the 5 most similar protocols

The effect of serotonin on radial growth was estimated by placing a mycelial plug (3 mm diameter) of the Fgdon⁺ strain in the center of a Petri dish (5.5 cm) containing PDA broth added with 0, 1 or 5 mM of serotonin (Sigma-Aldrich, Lyon, France). Radial growth was estimated after 24 and 48 h incubation at 26°C. To measure the impact on fungal sporulation, 1 ml of mung bean liquid medium containing a final concentration of 0, 1 or 5 mM serotonin was inoculated with 10⁴ conidia of the Fg DON⁺ strain and conidia were counted daily using a Thoma cell during one week. Conidia germination was performed on water agar (2%) containing serotonin (0, 1 or 5 mM). For each counting, the proportion of germinated conidia was estimated over a minimal total number of 100 conidia. Counting was performed 3 times for each serotonin concentration and for each incubation time after depositing conidia on the agar (3, 6 or 9 h).

Pasquet J.C., Chaouch S., Macadré C., Balzergue S., Huguet S., Martin-Magniette M.L., Bellvert F., Deguercy X., Thareau V., Heintz D., Saindrenan P, & Dufresne M. (2014). Differential gene expression and metabolomic analyses of Brachypodium distachyon infected by deoxynivalenol producing and non-producing strains of Fusarium graminearum. BMC Genomics, 15(1), 629.

+ Open protocol

+ Expand

Quantification of Serotonin Metabolites in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Charcoal stripped fetal bovine serum (FBS) was purchased from Atlanta Biologicals (Lawrenceville, GA, USA). Serotonin and N-acetylSerotonin were purchased from Sigma-Aldrich (St. Louis, MO, USA). HPLC grade acetonitrile, water and acetic acid (Fisher scientific, Pittsburgh, PA, USA) were used for HPLC. For LC-MS system, acetonitrile, water and formic acid (Sigma-Aldrich, St. Louis, MO, USA) were used. Dulbecco’s modified Eagle’s medium (DMEM) with high glucose (4,500 mg/L), 1% penicillin-streptomycin solution (10,000 units of penicillin and 10 mg of streptomycin in 1 mL 0.9% NaCl), DMSO, ethanol, HEPES (1 M), L-3,4-dihydroxyphenylalanine (L-DOPA), NaOH, non-essential amino acids (NEAA) (100×), RPMI-1640 medium, Serotonin, sodium pyruvate (100 mM), Triton^® X-100, were purchased from Sigma-Aldrich (St. Louis, MO, USA). FBS, 0.05% trypsin/0.53 mM EDTA solution, 1×PBS (pH 7.4), L-glutamine (200 mM) were supplied by Thermo Fisher Scientific (Waltham, MA, USA). Deuterated standard of N-acetylSerotonin [N-acetylSerotonin-d₇ (NAS-D₇)] was received from the National Institute of Mental Health Chemical Synthesis and Drug Supply Program NIMH # A-906, Bethesda, MD, USA.

Slominski A.T., Kim T.K., Kleszczyński K., Semak I., Janjetovic Z., Sweatman T., Skobowiat C., Steketee J.D., Lin Z., Postlethwaite A., Li W., Reiter R.J, & Tobin D.J. (2019). Characterization of Serotonin and N-acetylserotonin Systems in the Human Epidermis and Skin Cells. Journal of pineal research, 68(2), e12626.

+ Open protocol

+ Expand

Structural Characterization of XcAP-1-Serotonin Complex

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Purified recombinant XcAP-1 (19.8 mg/ml) was incubated with serotonin (Sigma, US) at 1:1.5 molar ratio (XcAP-1:serotonin) at 37 °C for 30 min. The complex was crystalized using the hanging drop vapor diffusion method in 0.1 M MES pH 5.4, 3.0 M (NH₄)₂SO₄ at room temperature. Mature crystals were obtained after a 7-day incubation period and were flash cooled in liquid nitrogen in 0.1 M MES pH 5.4, 3.7 M (NH₄)₂SO₄. Diffraction data were collected with the Southeast Regional Collaborative Access Team (SER CAT, Beamline 22-ID, Wavelength 1.000 Å) at the Advanced Photon Source (Argonne National Laboratory) and processed using HKL2000^{51 (link)}. The complex crystallized in the space group P2₁ with two complexes contained in the asymmetric unit (Table 2). The structure complex was solved by molecular replacement with Phaser from the CCP4i suit (8.0.006) using the XcAP-1 model structure predicted by AlphaFold2^{52 (link)}. The model was manually corrected using Coot (0.9.8.1)^{53 (link)} and refined with the phenix.refine (1.20.1)^{54 (link)} tool with a TLS model. Alphafold2 was also used to obtain tridimensional models of mature XcAP-2 and XcAP-3.

Lu S., Andersen J.F., Bosio C.F., Hinnebusch B.J, & Ribeiro J.M. (2023). Acid phosphatase-like proteins, a biogenic amine and leukotriene-binding salivary protein family from the flea Xenopsylla cheopis. Communications Biology, 6, 1280.

+ Open protocol

+ Expand

Transfection and Treatments of N1E-115 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The murine neuroblastoma cell line N1E-115 was cultured as previously described [14 (link)]. Transient transfection was performed using Lipofectamine 2000 reagent (Life Technologies) according to the manufacturer´s instructions. For transfection, we used plasmids encoding: eYFP, CDK5-eCFP, CDK5-mCherry, eGFP-Tau[R406W] as well as the above mentioned 5-HT7R WT and mutants under the control of a CMV promotor. For treatments, we applied 10 µM serotonin (Sigma), 50 µM 3-Isobutyl-1-methylxanthin (Tocris) and 5 µM forskolin (Sigma).

Ackmann J., Brüge A., Gotina L., Lim S., Jahreis K., Vollbrecht A.L., Kim Y.K., Pae A.N., Labus J, & Ponimaskin E. (2024). Structural determinants for activation of the Tau kinase CDK5 by the serotonin receptor 5-HT7R. Cell Communication and Signaling : CCS, 22, 233.

+ Open protocol

+ Expand

Melanoma Cell Lines: Cultivation and Compound Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols

B16F10, A375, SH4, RPMI-7951 and SK-MEL-24 melanoma cell lines were purchased from ATCC. MeWo and MEL-JUSO cell lines were kindly provided by Dr. A. Roesh (Universitätsklinikum Essen, Essen, Germany). The MEL-JUSO and MeWo cell lines were both originally purchased from ATCC. B16F10 murine cells, A375 and SH4 human malignant melanoma cell lines were maintained Dulbecco Modified Eagle’s Medium (DMEM). Human RPMI-7951 malignant melanoma cells were maintained in Eagle’s MEM. SK-MEL-24 were maintained in Eagle in Earle’s BSS with non-essential amino acids. MeWo and MEL-JUSO cell lines were maintained in Roswell Park Memorial Institute (RPMI) medium. All media were supplemented with 10% FCS (15% for SK-MEL-24) and penicillin streptomycin. Cells were incubated at 37 °C in 5% CO², and all cell lines were routinely confirmed to be mycoplasma-free (MycoAlert Mycoplasma Detection Kit, Lonza). The NIH Clinical Collection (NCC) composed of 770 small molecules mainly dissolved in DMSO at a concentration of 10 μM was obtained from the NIH, Tegaserod (Sigma) was dissolved in DMSO, serotonin (Sigma) was dissolved in water. MK-2206, ZSTK474, KU-0063794, Vemurafenib, Cobimetinib (Selleckchem) were dissolved in DMSO.

Liu W., Stachura P., Xu H.C., Umesh Ganesh N., Cox F., Wang R., Lang K.S., Gopalakrishnan J., Häussinger D., Homey B., Lang P.A, & Pandyra A.A. (2020). Repurposing the serotonin agonist Tegaserod as an anticancer agent in melanoma: molecular mechanisms and clinical implications. Journal of Experimental & Clinical Cancer Research : CR, 39, 38.

+ Open protocol

+ Expand

Potentiometric Neurotransmitter Sensor

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sodium tetrakis[3,5-bis(trifluoromethyl)phenyl]borate (NaTFPB), calix[4]arene (CX4), 2-nitrophenyl octyl ether (o-NPOE), high-molecular-weight poly(vinyl chloride) (PVC), tetrahydrofuran (THF, inhibitor-free, for HPLC), potassium hydrogen phosphate, potassium dihydrogen phosphate, D-(+)-Glucose monohydrate, potassium chloride, sodium chloride, calcium chloride, magnesium chloride, ammonium chloride, hydrochloric acid (HCl),

γ

-Aminobutyric acid (GABA), L-Glutamic acid monosodium salt hydrate, serotonin, dopamine, Acetylcholinesterase from Electrophorus electricus (AChE), and OmniPur Tris HCl buffer were purchased from Sigma Aldrich. We prepared all stock solutions with MiliQ deionized water (resistivity of 18.2 MΩ·cm) unless stated otherwise. The sheep brain tissue was purchased from a local market (Tehran Market, Los Angeles, CA, USA).

Amirghasemi F., Soleimani A., Bawarith S., Tabassum A., Morrel A, & Mousavi M.P. (2023). FAST (Flexible Acetylcholine Sensing Thread): Real-Time Detection of Acetylcholine with a Flexible Solid-Contact Potentiometric Sensor. Bioengineering, 10(6), 655.

+ Open protocol

+ Expand

Vasoactive Compound Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serotonin, U46619, quinacrine, indomethacin, and SQ29548 were obtained from Sigma-Aldrich and dissolved on the day of the study.

Lawandy I., Liu Y., Shi G., Zhang Z., Scrimgeour L.A., Pavlov V., Jaworski R., Sellke F.W, & Feng J. (2019). Increased coronary arteriolar contraction to serotonin in juvenile pigs with metabolic syndrome. Molecular and Cellular Biochemistry, 461(1), 57-64.

+ Open protocol

+ Expand

Aβ-induced paralysis in transgenic worms

Check if the same lab product or an alternative is used in the 5 most similar protocols

After egg-synchronized, the transgenic snb-1/Aβ_1-42 worm were placed at 20 °C on NGM plates seeded with OP50 for 48 h. The worms were treated with 0, 1 mg/mL, 5 mg/mL, 15 mg/mL DXN for another 48 h, respectively. 30 worms in each group were washed with M9 buffer for three times and were transferred into 200 μL M9 buffer containing 1 mg serotonin (Sigma), paralyzed worms were scored after 5 min. Animals were considered to be paralyzed if they did not move at all within 5 sec. Worm strain CL2122 without Aβ expression in nerve cells was used as a transgenic control.

Zhi D., Wang D., Yang W., Duan Z., Zhu S., Dong J., Wang N., Wang N., Fei D., Zhang Z., Wang X., Wang M, & Li H. (2017). Dianxianning improved amyloid β-induced pathological characteristics partially through DAF-2/DAF-16 insulin like pathway in transgenic C. elegans. Scientific Reports, 7, 11408.

+ Open protocol

+ Expand

Aporphine Derivatives: Sourcing and Applications

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aporphine derivatives were obtained from the vendors indicated: R-(−)-apomorphine (Sigma Aldrich, A4393), (−)nuciferine (Cerilliant, PHY83282), D-Glaucine (Santa Cruz Biotechnology, sc-490895), (+)boldine (Sigma Aldrich, 67592), (+)-Bulbocapnine (Santa Cruz Biotechnology, sc-257199). Other chemicals sourced for assays were 3-Isobutyl-1-methylxanthine (Sigma Aldrich, I5879), serotonin (Sigma Aldrich, H9523) and forskolin (Cell Signaling Technology, 3828).

Chan J.D., Acharya S., Day T.A, & Marchant J.S. (2016). Pharmacological profiling an abundantly expressed schistosome serotonergic GPCR identifies nuciferine as a potent antagonist. International Journal for Parasitology: Drugs and Drug Resistance, 6(3), 364-370.

+ Open protocol

+ Expand

Mass Spectrometry Analysis of Neurotransmitters

Check if the same lab product or an alternative is used in the 5 most similar protocols

6-OHDA hydrochloride, MPP⁺ iodide, chloral hydrate, ketamine, xylazine, paraformaldehyde, MPTP, dopamine, serotonin, homovanillic acid (HVA), 3,4-dihydroxyphenylacetic acid (DOPAC), ascorbic acid, and [²H₅]-benzoyl chloride were purchased from Sigma Aldrich (Saint Louis, MO). [²H₄]-PEA and [²H₄]-OEA were from Cayman Chemical (Ann Arbor, MI, USA). Tandem Mass Tag™ 6-plex (TMTsixplex™) reagents kits for isotopic labeling were from Thermo Fisher Scientific (Waltham, MA, USA). All analytical solvents were of the highest grade, and were obtained from Honeywell (Muskegon, MI) or Sigma-Aldrich. ARN19702 was synthetized as described [8 (link)].

Palese F., Pontis S., Realini N., Torrens A., Ahmed F., Assogna F., Pellicano C., Bossù P., Spalletta G., Green K, & Piomelli D. (2022). Targeting NAAA counters dopamine neuron loss and symptom progression in mouse models of parkinsonism. Pharmacological research, 182, 106338.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!