Mk111

Manufactured by Takara Bio

Sourced in Japan

The MK111 is a laboratory instrument used for the amplification and detection of nucleic acids. It is designed to perform real-time quantitative PCR (qPCR) analysis. The core function of the MK111 is to precisely measure and analyze the levels of specific DNA or RNA sequences within a sample.

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Lab products found in correlation

Mk118, by Takara Bio (3 mentions) Cobas e601, by Roche (1 mentions) Beckman au5800 automatic biochemical analyzer, by Beckman Coulter (1 mentions) Advia centaur, by Siemens (1 mentions)

4 protocols using mk111

Bone Turnover Markers Serum Analysis

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ELISA kits were used to measure the serum levels of human bone Alkaline Phosphatase (bALP; Cusabio, CSB-E09033h, Houston, TX, USA), procollagen I N-terminal Peptide (PINP; Cusabio, CSB-E11226h), carboxyterminal propeptide of type I procollagen (PICP; Cusabio, CSB-E08079h), cross linked N-telopeptide of type I collagen (NTX; Cusabio, CSB-E09233h), and cross linked C-telopeptide of type I collagen (CTX; Novusbio, NBP2-69073, Centennial, CO, USA).
EIA kits were used to measure serum levels of human osteocalcin in the undercaboxylated (unOC) and carboxylated (cOC) form (Takara, Shiga, Japan, MK-118 and MK-111, respectively).

Mandatori D., Penolazzi L., Pelusi L., Lambertini E., Michelucci F., Porreca A., Cerritelli P., Pipino C., Di Iorio A., Bruni D., Di Nicola M., Buda R., Piva R, & Pandolfi A. (2021). Three-Dimensional Co-Culture System of Human Osteoblasts and Osteoclast Precursors from Osteoporotic Patients as an Innovative Model to Study the Role of Nutrients: Focus on Vitamin K2. Nutrients, 13(8), 2823.

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Bovine Osteocalcin Concentration Analysis

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Cows were randomly selected within KET and CON groups, respectively, for the analysis of cOC (n = 16 in KET and n = 15 in CON) and ucOC (n = 17 in KET and n = 18 in CON) concentrations, and plasma samples from BW–1 and BW1 were used. The analysis was performed using cOC and ucOC ELISA kits (MK111 and MK118, Takara, Shiga, Japan), and the intra-and inter-assay coefficients of variation (CV) were 4.3 and 3.6% for cOC and 5.9 and 7.8% for ucOC, respectively.

Choi W., Ro Y., Choe E., Hong L., Kim D., Kim S., Yoon I, & Kim D. (2023). Comparison of prepartum blood parameters in dairy cows with postpartum ketosis and new risk prediction candidates. Frontiers in Veterinary Science, 10, 1161596.

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Acute Stress Bioactive Osteocalcin Measurement

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The acute stress was carried out as previously described (Prather et al., 2009 (link)) under University of Pittsburgh IRFB -- #0307004. Consent was obtained and subjects of both sexes were aged between 41-58 years and were in good health with no record of serious illness sample size was determined based on previously performed studies (Prather et al., 2009 (link)). In short, subjects engaged in a 10-minute evaluative speech task and blood was sampled before, immediately after and 30 minutes after the completion of the test. Circulating levels of bioactive osteocalcin in humans was estimated using a bioactive osteocalcin ratio according to a recently published method (Bonneau et al., 2017) that has greatly improved the accuracy of detecting undercarboxylated osteocalcin levels in human serum to within a 4.5-5.7% coefficient of variability. For human samples, separate ELISAs are used to measure total osteocalcin levels (ALPCO, 38-OSTHU-E01) and carboxylated osteocalcin levels (Takara Bio, MK111). Briefly, total and fully carboxylated osteocalcin levels were normalized to pre-stress levels and then undercarboxylated osteocalcin level was estimated for each participant by dividing Gla17-OCN by total-OCN.

Berger J.M., Singh P., Khrimian L., Morgan D.A., Chowdhury S., Arteaga-Solis E., Horvath T.L., Domingos A.I., Marsland A.L., Yadav V.K., Rahmouni K., Gao X.B, & Karsenty G. (2019). Mediation of the acute stress response by the skeleton. Cell metabolism, 30(5), 890-902.e8.

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Serum Biomarkers Assessment Protocol

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Blood sample were harvested in the morning after at least 8 hours fasting. Serum was gathered after centrifugation at 3,000 r/min for 10 minutes. Serum ucOC were assayed by ELISA (MK118, Takara Bio Inc, Japan), with the intra assay coe cient of variation of 4.58%-6.66% and the inter assay coe cient of variation of 5.67%-9.87%. Serum cOC were measured by ELISA (MK111, Takara Bio Inc, Japan), with the intra assay coe cient of variation of 3.3%-4.8%, the inter assay coe cient of variation of 0.7%-2.4%.
Serum calcium (Ca), phosphorus (Pi), and alkaline phosphatase (ALP) measurements were performed by a Beckman Automatic Biochemical Analyzer (AU5800, Beckman Coulter, Indianapolis, IN, USA). Serum OC, β-isomer of C-terminal telopeptides of type I collagen (β-CTX), N-terminal prepeptide of type 1 procollagen (P1NP) were measured by electrochemiluminescence immunoassay (Roche Cobas e601, Mannheim, Germany). Parathyroid hormone (PTH) was measured using chemiluminescence (Siemens ADVIA Centaur, Munich, Germany). 25hydroxyvitamin D (25OHD) was measured by electrochemiluminescence immunoassay (Roche Cobas e601, Mannheim, Germany). Creatinine (Cr) was measured by an automated Roche electrochemiluminescence system (E170; Roche Diagnostics, Basel, Switzerland). All measurements were completed by central laboratory of PUMCH.

Liu S., Pang Q., Guan W., Yu F., Wang O., Li M., Xing X., Yu W., Jiang Y, & Xia W. (2024). Association of serum osteocalcin with bone microarchitecture and muscle mass in Beijing community-dwelling postmenopausal women. Endocrine, 84(1).

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