Kidney tissues were subjected to western blot analysis using a procedure described previously.24 After blocking nonspecific binding with 5% nonfat milk in phosphate‐buffered saline solution or 1 h at room temperature, membranes were incubated with the primary antibodies overnight at 4°C. The primary antibodies include anti‐NDUFV1 antibody (PA5‐98007; Invitrogen), anti‐SDHA antibody (11,998; Cell Signalling Technology), anti‐HSP60 antibody (12,165; Cell Signalling Technology), anti‐PHB1 antibody (2426; Cell Signalling Technology), anti‐VDHC antibody (4661; Cell Signalling Technology), anti‐Cox IV antibody (4850; Cell Signalling Technology), anti‐Bax antibody (2772; Cell Signalling Technology), anti‐Bcl‐2 antibody (3498; Cell Signalling Technology), anti‐cleaved Caspase 3 antibody (9664; Cell Signalling Technology), and anti‐Actin antibody (3700; Cell Signalling Technology). After three times washing in Tris‐buffered saline with Tween (TBST), membranes were then incubated with an appropriate secondary antibody for 1 h at room temperature. Membranes were developed using a chemiluminescence reagent. The Image J software was used to analyse the densitometry of the blots.
Anti hsp60 antibody
Manufactured by Cell Signaling Technology
The Anti-HSP60 antibody is a laboratory reagent used in research applications to detect the presence and abundance of the heat shock protein 60 (HSP60) in biological samples. HSP60 is a chaperone protein involved in the folding and transport of other proteins within cells. This antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to study the expression and localization of HSP60 in various cell types and tissues.
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Lab products found in correlation
Anti sdha antibody, by Cell Signaling Technology (1 mentions)
Anti cox 4 antibody, by Cell Signaling Technology (1 mentions)
Anti bax antibody, by Cell Signaling Technology (1 mentions)
Anti bcl 2 antibody, by Cell Signaling Technology (1 mentions)
Anti actin antibody, by Cell Signaling Technology (1 mentions)
Anti cleaved caspase 3 antibody, by Cell Signaling Technology (1 mentions)
Alexa 647, by Thermo Fisher Scientific (1 mentions)
Tcs sp8 confocal microscope, by Leica (1 mentions)
Alexa 555, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti hsp60 antibody
1
Western Blot Analysis of Kidney Proteins
2
Immunofluorescence Localization of Proteins
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Cells were fixed with 4% paraformaldehyde before treatment with 0.2% Triton-PBS for 15 min. Samples were then washed with PBST (0.1% Tween, PBS) and incubated with blocking solution (1% bovine serum albumin in PBST) for 1 h at room temperature, followed by incubation with anti-Myc antibody (MBL) or anti-HSP60 antibody (Cell Signaling Technology) at 4°C overnight. After incubation, the cells were washed with PBST 2 times and incubated with the secondary antibodies Alexa 555 and Alexa 647 (Invitrogen) for 1 h at room temperature. The samples were then incubated with DAPI for 10 min to stain the nuclei. The cells were analyzed using a TCS SP8 confocal microscope (Leica).
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