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Lipase activity assay kit 3

Manufactured by Merck Group
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The Lipase Activity Assay Kit III is a laboratory equipment product designed to measure the activity of the enzyme lipase. It provides a colorimetric method for quantifying lipase levels in various samples. The kit includes the necessary reagents and buffers to perform the assay.

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Lab products found in correlation

Lpl activity assay kit, by Merck Group (1 mentions) Pltp activity assay kit, by Merck Group (1 mentions) Pierce fluorescent protease assay kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Lipase Activity Kit Activity Assay Kits Lipase

4 protocols using lipase activity assay kit 3

1

Lipoprotein Lipase Activity Assay

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LPL activity was determined with a 50 μl aliquot of culture medium after 30 min of treatment with 50 units of heparin using an LPL activity assay kit (Sigma) according to the manufacturer's instructions. Intracellular total lipase activity was measured using a lipase activity assay kit III (Sigma).
Barilla S., Liang N., Mileti E., Ballaire R., Lhomme M., Ponnaiah M., Lemoine S., Soprani A., Gautier J.F., Amri E.Z., Le Goff W., Venteclef N, & Treuter E. (2020). Loss of G protein pathway suppressor 2 in human adipocytes triggers lipid remodeling by upregulating ATP binding cassette subfamily G member 1. Molecular Metabolism, 42, 101066.
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2

Enzyme Activity Assays in Bacillus odoriphaga

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100mg B. odoriphaga collected at three developmental stages, respectively, was used to assay the activity of enzymes. There biological replicates were designed for this study. According to the NR annotation of 45 genes selected randomly in the common differential expression of genes among three comparisons, ten DEGs that could be expressed as eight functional enzymes were selected to validate the gene expression profiles. Pectate lyase (PEL) activity and glucose dehydrogenase (GLD) activity were assayed as described by Kovtunovych et al. [28 ] and Fender et al. [29 (link)], respectively. The activity of glutathione s-transferase (GST) was detected according to Glutathione S-Transferase Fluorescent Activity Kit (Enzo life science, USA). The assay of Carboxylesterase (CarE) activity referenced the methods of Zou et al. [30 (link)]. Alpha-Amylase Assay Kit (Abnova, USA), Lipase Activity Assay Kit III (Sigma, USA), Nitric Oxide Synthase Assay Kit (Antibodies-online, Germany), Fatty Acid Synthase assay Kit (Solarbio, China) were used to assayed the activity of alpha-amylase (AA), lipase (Lip), nitric oxide synthase (NOS) and fatty acid synthase (FAS).
Gao H., Zhai Y., Wang W., Chen H., Zhou X., Zhuang Q., Yu Y, & Li R. (2016). Transcriptome Analysis and Discovery of Genes Relevant to Development in Bradysia odoriphaga at Three Developmental Stages. PLoS ONE, 11(2), e0146812.
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3

Serum Enzyme Activity Assays

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Serum enzyme activities were determined using a commercially available kit. CETP enzyme activity was assayed using a cell-free assay according to the manufacturer’s protocol (Roar Biomedical).
LCAT activity assays were performed with an LCAT Activity Assay Kit (Fluorometric) from Cell Biolabs (Cat# STA-615). Whole-serum lipase activity was evaluated using Lipase Activity Assay Kit III from Sigma-Aldrich (Cat# MAK048), and PLTP activity assays were performed using PLTP Activity Assay Kit from Sigma-Aldrich (Cat# MAK108).
Zhu L., Luu T., Emfinger C.H., Parks B.A., Shi J., Trefts E., Zeng F., Kuklenyik Z., Harris R.C., Wasserman D.H., Fazio S, & Stafford J.M. (2018). CETP Inhibition Improves HDL Function but Leads to Fatty Liver and Insulin Resistance in CETP-Expressing Transgenic Mice on a High-Fat Diet. Diabetes, 67(12), 2494-2506.
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4

Quantifying Lipase and Protease Activities

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Total lipase activity was estimated using the fluorometric Lipase Activity Assay Kit III (Sigma-Aldrich). Total protease concentrations were estimated using the Pierce Fluorescent Protease Assay Kit (Thermo Fisher Scientific). In each case, fluorescence was measured using a Tecan Infinite200 plate spectrophotometer. Lipase and protease activity were also tested visually by agar diffusion assays (SI Appendix, SI Methods).
Shukla S.P., Plata C., Reichelt M., Steiger S., Heckel D.G., Kaltenpoth M., Vilcinskas A, & Vogel H. (2018). Microbiome-assisted carrion preservation aids larval development in a burying beetle. Proceedings of the National Academy of Sciences of the United States of America, 115(44), 11274-11279.
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