HUVEC, HPAEC, or HMVEC-L were seeded at 4 × 104 cells per well onto EMD Millipore Millicell EZ Slides (PEZGS0416; Sigma Millipore), grown in complete medium for 48 hours, and subsequently serum starved for 16 hours. After receiving inhibitors and/or human recombinant VEGF-165 stimulation, cells were fixed in 4% paraformaldehyde (Boston Bioproducts), permeabilized in cold methanol (Fisher), and immunofluorescence staining was performed. Nuclei were stained using 4’, 6-diamidino-2-phenylindole (DAPI; Cell Signaling; Catalog No. 8961S). All images were captured on a Zeiss Apotome 2 microscope by using 20×, 0.8 NA Plan Apochromat objective magnifying 2X to 4X and processed using ImageJ software (Version 1.8.0_112; https://imagej.nih.gov/ij ).
Emd millipore millicell ez slides
Manufactured by Merck Group
The EMD Millipore Millicell EZ Slides are a type of laboratory equipment used for cell culture applications. The slides provide a transparent, permeable membrane that allows for the growth and study of cells in a controlled environment.
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2 protocols using emd millipore millicell ez slides
1
VEGF-Induced Endothelial Cell Imaging
2
VEGF-induced Endothelial Cell Imaging
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HUVECs, HPAECs or HMVEC-Ls were seeded at 4×104 cells per well onto EMD Millipore Millicell EZ Slides (PEZGS0416, Sigma Millipore), grown in complete medium for 48 h and subsequently serum starved for 16 h. After receiving inhibitors and/or human recombinant VEGF-165 stimulation, cells were fixed in 4% paraformaldehyde (Boston Bioproducts), permeabilized in cold methanol (Thermo Fisher Scientific), and immunofluorescence was performed. Nuclei were stained using 4′, 6-diamidino-2-phenylindole (DAPI; 8961S, Cell Signaling Technology). All images were captured on a Zeiss Apotome 2 microscope using a 20×/0.8 NA Plan Apochromat objective magnifying 2× to 4× and processed using ImageJ software.
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