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Goat anti mouse igm af488 secondary

Manufactured by Thermo Fisher Scientific

Goat anti-mouse IgM AF488 secondary is a fluorescently labeled secondary antibody used for detecting and visualizing mouse IgM primary antibodies in various immunoassays and imaging applications.

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2 protocols using goat anti mouse igm af488 secondary

1

Immunofluorescence Staining Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
For Figures 4I and J, immunofluorescence was conducted as published12 (link), with using anti-TFAP2C (SantaCruz 8977, 1:100), anti-OCT4 (sc8628-X, 1:100), anti-PAX6 (R&D Systems AF3369, 1:100). For Supplementary Figure 6F, immunostaining was performed as previously described51 (link). Primary antibodies used: rabbit anti-KLF17 polyclonal (1:500, Sigma HPA024629), mouse anti-TRA-1-60 IgM (1:300, BD), and secondary antibodies used: goat anti-rabbit IgG AF555 secondary (1:400, ThermoFisher), goat anti-mouse IgM AF488 secondary (1:400, ThermoFisher).
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2

Immunofluorescence Staining Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
For Figures 4I and J, immunofluorescence was conducted as published12 (link), with using anti-TFAP2C (SantaCruz 8977, 1:100), anti-OCT4 (sc8628-X, 1:100), anti-PAX6 (R&D Systems AF3369, 1:100). For Supplementary Figure 6F, immunostaining was performed as previously described51 (link). Primary antibodies used: rabbit anti-KLF17 polyclonal (1:500, Sigma HPA024629), mouse anti-TRA-1-60 IgM (1:300, BD), and secondary antibodies used: goat anti-rabbit IgG AF555 secondary (1:400, ThermoFisher), goat anti-mouse IgM AF488 secondary (1:400, ThermoFisher).
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