Immunofluorescence assays were performed as described previously36 (link). The primary antibody, anti-Ki67 (sc-23900), was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-FHL2 (ab12327) was obtained from Abcam (Cambridge, UK). Anti-β-catenin (#8480) was obtained from Cell Signaling Technology (Danvers, MA, USA). The fluorescein isothiocyanate (FITC)-conjugated donkey anti-mouse (D110081-0100) and Cy3-conjugated donkey anti-rabbit (D110052-010) secondary antibodies were obtained from Sangon Biotech (Shanghai, China).
Anti β catenin 8480
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-β-catenin (#8480) is a primary antibody product from Cell Signaling Technology. The antibody recognizes β-catenin, a key mediator of the Wnt signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Anti ki67 sc 23900, by Santa Cruz Biotechnology (1 mentions)
β tubulin 10094 1 ap, by Proteintech (1 mentions)
Anti mouse igg hrp ap308p, by Merck Group (1 mentions)
Anti c myc ht101, by Transgene (1 mentions)
Anti lmnb1 101237 t32, by Sino Biological (1 mentions)
Hrp anti hemagglutinin, by Roche (1 mentions)
Anti stub1 12496 r034, by Sino Biological (1 mentions)
Anti β tubulin hc101, by Transgene (1 mentions)
4 protocols using anti β catenin 8480
1
Immunofluorescence Assay Protocol for Cell Markers
2
Western Blot Analysis of Protein Markers
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Total protein was extracted from 1 × 106 cells, and the protein concentration was determined by the BCA assay. 20 μg of protein sample solution was used for western blot analysis; the primary antibodies, anti-ANTXR1 (ab21270), anti-fetal hemoglobin (ab137096), anti-hemoglobin subunit beta antibody (ab214049), anti-LRP6 (ab75358), anti-cyclin D1 (ab40754), anti-GAPDH (ab181602), anti-GFP (ab290), anti-Flag antibody (ab205606) from Abcam, and anti-β-catenin (#8480) and anti c-Jun (#9165s) were purchased from Cell Signaling Technology. The membrane was then washed three times with TBST, the secondary HRP-conjugated antibody was applied, and the membrane was incubated for 1 hour at room temperature. Finally, immunoreactive protein bands were visualized using the ECL system, and the optical density of the protein bands was quantified using ImageJ software (http://imagej.nih.gov/ij ).
3
Antibody Validation for Western Blotting
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Anti-ME2 (ab139686; dilution: 1/1000) was purchased from Abcam (Cambridge, UK). Antibodies of β-actin (66009-1; dilution: 1/3000) and β-tubulin (10094-1-AP; dilution: 1/3000) were from Proteintech (Chicago, IL, USA). The anti-β-catenin (8480; dilution: 1/1000) was ordered from Cell Signaling Technology (Beverly, MA, USA). Dimethyl pyruvate (371173) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
4
Wnt-3a Signaling Pathway Regulation
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Recombinant Human Wnt-3a (NP-149122) was purchased from an R&D system (Minneapolis, MN, USA).
Horseradish peroxidase (HRP)-anti-Flag (M2) (A8592), anti-β-actin (A1978), anti-rabbit-IgG-HRP (AP132P), and anti-mouse-IgG-HRP (AP308P) were purchased from Sigma (St. Louis, MO, USA). HRP-anti-hemagglutinin (12013819001) was purchased from Roche Applied Science (Switzerland, Basel). anti-c-Myc (HT101) and anti-β-tubulin (HC101) were purchased from TransGen Biotech (Beijing, China). anti-β-Catenin (8480) and anti-non-phospho β-Catenin (19807) were purchased from Cell Signaling Technology (Danvers, MA, USA). anti-LMNB1 (101237-T32) and anti-STUB1 (12496-R034) were purchased from Sino Biological (Beijing, China). anti-LEF1 (sc-374522) and anti-Arc (sc-17839) were purchased from Santa Cruz Biotechnology (San Diego, CA, USA).
Empty vector pcDNA3.1 was kindly provided by Dr Jun Cui (Sun Yat-sen University, Guangzhou, China). Target genes were cloned from A549 cDNA and subcloned into the pcDNA3.1 vector. The plasmids were confirmed by DNA sequencing at Sangon Biotech (Shanghai, China).
Horseradish peroxidase (HRP)-anti-Flag (M2) (A8592), anti-β-actin (A1978), anti-rabbit-IgG-HRP (AP132P), and anti-mouse-IgG-HRP (AP308P) were purchased from Sigma (St. Louis, MO, USA). HRP-anti-hemagglutinin (12013819001) was purchased from Roche Applied Science (Switzerland, Basel). anti-c-Myc (HT101) and anti-β-tubulin (HC101) were purchased from TransGen Biotech (Beijing, China). anti-β-Catenin (8480) and anti-non-phospho β-Catenin (19807) were purchased from Cell Signaling Technology (Danvers, MA, USA). anti-LMNB1 (101237-T32) and anti-STUB1 (12496-R034) were purchased from Sino Biological (Beijing, China). anti-LEF1 (sc-374522) and anti-Arc (sc-17839) were purchased from Santa Cruz Biotechnology (San Diego, CA, USA).
Empty vector pcDNA3.1 was kindly provided by Dr Jun Cui (Sun Yat-sen University, Guangzhou, China). Target genes were cloned from A549 cDNA and subcloned into the pcDNA3.1 vector. The plasmids were confirmed by DNA sequencing at Sangon Biotech (Shanghai, China).
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