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Prepx polya mrna isolation kit

Manufactured by Takara Bio
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The PrepX PolyA mRNA Isolation Kit is a laboratory tool designed for the extraction and purification of polyadenylated messenger RNA (mRNA) from various biological samples. The kit utilizes magnetic beads coated with oligo(dT) sequences to selectively capture and isolate the poly(A) tails of mRNA molecules, allowing for their separation from other cellular components.

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Lab products found in correlation

Prepx rna seq for library kit, by Illumina (4 mentions) Tapestation 2200, by Agilent Technologies (4 mentions) Hiseq 2500 high output v3 flow cell, by Illumina (3 mentions) Longamp taq 2x master mix, by New England Biolabs (2 mentions) Longamp taq 2 master mix, by New England Biolabs (2 mentions) Nextseq 500 mid output flow cell, by Illumina (1 mentions)

4 protocols using prepx polya mrna isolation kit

1

Automated RNA-Seq Library Preparation

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Polyadenylated mRNAs were selected from total RNA samples using oligo-dT-conjugated magnetic beads on an Apollo324 automated workstation (PrepX PolyA mRNA isolation kit, Takara Bio USA). Entire poly-adenylated RNA samples were immediately converted into stranded Illumina sequencing libraries using 200 bp fragmentation and sequential adapter addition on an Apollo324 automated workstation following manufacturer’s specifications (PrepX RNA-Seq for Illumina Library kit, Takara Bio USA). Libraries were enriched and indexed using 12 cycles of amplification (LongAmp Taq 2x MasterMix, New England BioLabs Inc.) with PCR primers which include a 6 bp index sequence to allow for multiplexing (custom oligo order from Integrated DNA Technologies). Excess PCR reagents were removed using magnetic bead-based cleanup on an Apollo324 automated workstation (PCR Clean DX beads, Aline Biosciences). Resulting libraries were assessed using a 2200 TapeStation (Agilent Technologies) and quantified by QPCR (Kapa Biosystems). Libraries were pooled and sequenced on one lane of a HiSeq2500 high output v3 flow cell using single end, 50 bp reads (Illumina).
Park H.R., O’Sullivan M., Vallarino J., Shumyatcher M., Himes B.E., Park J.A., Christiani D.C., Allen J, & Lu Q. (2019). Transcriptomic response of primary human airway epithelial cells to flavoring chemicals in electronic cigarettes. Scientific Reports, 9, 1400.
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2

RNA-Seq Library Preparation Automated

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polyadenylated mRNAs were selected from total RNA samples using oligo-dT-conjugated magnetic beads on an Apollo324 automated workstation (PrepX PolyA mRNA isolation kit, Takara Bio USA). Entire poly-adenylated RNA samples were immediately converted into stranded Illumina sequencing libraries using 200bp fragmentation and sequential adapter addition on an Apollo324 automated workstation following manufacturer’s specifications (PrepX RNA-Seq for Illumina Library kit, Takara Bio USA). Libraries were enriched and indexed using 12 cycles of amplification (LongAmp Taq 2× MasterMix, New England BioLabs Inc.) with PCR (polymerase chain reaction) primers which included a 6bp index sequence to allow for multiplexing (custom oligo order from Integrated DNA Technologies). Excess PCR reagents were removed using magnetic bead-based cleanup (PCR Clean DX beads, Aline Biosciences). RNA integrity was checked with a 2200 TapeStation (Agilent Technologies), and after libraries were created, they were quantified by QPCR (Kapa Biosystems). Libraries were pooled and sequenced on one lane of a HiSeq 2500 high output v3 flow cell using single end, 50 bp reads (Illumina).
Singh D., Marrocco A., Wohlleben W., Park H.R., Diwadkar A.R., Himes B.E., Lu Q., Christiani D.C, & Demokritou P. (2021). Release of particulate matter from nano-enabled building materials (NEBMs) across their lifecycle: potential occupational health and safety implications. Journal of hazardous materials, 422, 126771.
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3

Automated RNA-Seq Library Preparation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Libraries for sequencing were prepared as previously described29 (link). Polyadenylated mRNAs were selected from total RNA samples using oligo-dT-conjugated magnetic beads on an Apollo324 automated workstation (PrepX PolyA mRNA isolation kit, Takara Bio USA). Entire poly-adenylated RNA samples were immediately converted into stranded Illumina sequencing libraries using 200 bp fragmentation and sequential adapter addition on an Apollo324 automated workstation following manufacturer’s specifications (PrepX RNA-Seq for Illumina Library kit, Takara Bio USA). Libraries were enriched and indexed using 12 cycles of amplification (LongAmp Taq 2 × MasterMix, New England BioLabs Inc.) with PCR primers which include a 6 bp index sequence to allow for multiplexing (custom oligo order from Integrated DNA Technologies). Excess PCR reagents were removed using magnetic bead-based cleanup on an Apollo324 automated workstation (PCR Clean DX beads, Aline Biosciences). Resulting libraries were assessed using a 2200 TapeStation (Agilent Technologies) and quantified by QPCR (Kapa Biosystems). Libraries were pooled and sequenced on one lane of a HiSeq 2500 high output v3 flow cell using single end, 50 bp reads (Illumina).
Park H.R., Vallarino J., O’Sullivan M., Wirth C., Panganiban R.A., Webb G., Shumyatcher M., Himes B.E., Park J.A., Christiani D.C., Allen J, & Lu Q. (2021). Electronic cigarette smoke reduces ribosomal protein gene expression to impair protein synthesis in primary human airway epithelial cells. Scientific Reports, 11, 17517.
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4

RNA-seq Library Preparation and Sequencing

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mRNA was extracted from the isolated buds and bulks using Direct-zol RNA mini kit (Thermo Fisher Scientific). Polyadenylated mRNAs were selected from 500ng total RNA samples using oligo-dT-conjugated magnetic beads on an Apollo324 automated workstation (PrepX PolyA mRNA isolation kit, Takara Bio USA). Entire poly-adenylated RNA samples were immediately converted into stranded Illumina sequencing libraries using 200bp fragmentation and sequential adaptor addition on an Apollo324 automated workstation following manufacturer’s specifications (PrepX RNA-seq for Illumina Library kit, Takara Bio USA). Libraries were enriched and indexed using 14 cycles of amplification (LongAmpTaq 2x MasterMix, New England BioLabs Inc.) with PCR primers which included a 6bp index sequence to allow for multiplexing (custom oligo order from Integrated DNA Technologies). Excess PCR reagents were removed using magnetic bead-based cleanup on an Apollo324 automated workstation (PCR Clean DX beads, Aline Biosciences). Resulting libraries were assessed using a 2200 TapeStation (Agilent Technologies) and quantified by qPCR (Kapa Biosystems). Libraries were pooled and sequenced on an Illumina NextSeq 500 mid output flow cell using single end, 75bp reads.
Sharon N., Chawla R., Mueller J., Vanderhooft J., Whitehorn J.L., Rosenthal B., Gürtler M., Estanboulieh R.R., Shvartsman D., Gifford D.K., Trapnell C, & Melton D. (2019). A Peninsular Structure Coordinates Asynchronous Differentiation with Morphogenesis to Generate Pancreatic Islets. Cell, 176(4), 790-804.e13.
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