Measurements of steroid levels were done on GCMS-QP2010 Plus system by Shimadzu (Kyoto, Japan) consisting of a gas chromatograph equipped with automatic flow control, an AOC-20s autosampler, and a single quadrupole detector with an adjustable electron voltage of 10-195 V. A capillary column with a medium polarity RESTEK Rxi TM -50 (diameter 0.25 mm, length 15 m, film thickness 0.1 μm) was used for analyses. Electron impact ionization with electron voltage fixed at 70 V and emission current set to 160 μA was used. The temperatures of the injection port, ion source and interface were maintained at 220 °C, 300 °C, and 310 °C, respectively. Analyses were carried out in the splitless mode with a constant linear velocity of the carrier gas (He), which was maintained at 60 cm/s. The septum purge flow was set at 3 ml/min. The samples were injected using the high pressure mode (200 kPa), which was maintained for 1 min. The detector voltage was set to 1.2 kV.
Gcms qp2010 plus system
Manufactured by Shimadzu
Sourced in Japan
The GCMS-QP2010 Plus system is a gas chromatograph-mass spectrometer from Shimadzu. It is designed for the analysis and identification of a wide range of chemical compounds. The system combines a gas chromatograph (GC) with a quadrupole mass spectrometer (MS) to separate, detect, and identify substances in complex mixtures.
Automatically generated - may contain errors
Lab products found in correlation
Aoc 20s autosampler, by Shimadzu (4 mentions)
Rxi phase, by Shimadzu (3 mentions)
Aoc 20s autosampler, by Restek (2 mentions)
Gcms solution ver 2.50su3, by Shimadzu (2 mentions)
Zb 5ms fused silica, by Phenomenex (2 mentions)
Gcmssolution software, by Shimadzu (2 mentions)
β caryophyllene, by Extrasynthese (1 mentions)
Dodecane, by Merck Group (1 mentions)
β caryophyllene, by Merck Group (1 mentions)
Gc 2010 plus, by Shimadzu (1 mentions)
20 ml headspace vial, by Agilent Technologies (1 mentions)
Tq8040 mass spectrometer, by Shimadzu (1 mentions)
Rtx 5ms column, by Restek (1 mentions)
Trimethylchlorosilane, by Thermo Fisher Scientific (1 mentions)
2 isopropylmalic acid, by Merck Group (1 mentions)
Db 5 column, by Agilent Technologies (1 mentions)
Gc solution software, by Shimadzu (1 mentions)
Rtx 5ms capillary column, by Restek (1 mentions)
Equity 5 column, by Merck Group (1 mentions)
Equity 5 capillary column, by Merck Group (1 mentions)
35 protocols using gcms qp2010 plus system
1
Steroid Quantification by GC-MS
2
GC-MS Analysis of Rhododendron arboreum Leaves
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The hexane, chloroform, and ethyl acetate fractions of R. arboreum leaves were analyzed using a GCMS-QP2010 Plus system (Shimadzu, Kyoto, Japan). The instrument conditions and parameters used for analysis were as follows: Column oven temperature: 70 °C for first 5 min; increased to 250 °C at a rate of 10 °C/min and held for next 10 minutes; further increased to 300°C at a rate of 10 °C/min and held at this temperature for 10 min. Carrier gas: Helium. Injection temperature: 280 °C. Injection mode: Splitless. Sampling time: 1 min. Pressure: 110.8 kPa. Total flow: 38.9 mL/min. Column flow: 1.71 mL/min. Linear velocity: 47.9 cm/sec. Purge flow: 3 mL/min. All the discovered compounds were recognized by evaluating their mass spectra with those listed in the National Institute of Standard and Technology (NIST08s) and Wiley 7 libraries.
3
GC-MS Analysis of Organic Compounds
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A GCMS-QP2010 Plus system from Shimadzu (Kyoto, Japan) consisting of a gas chromatograph equipped with automatic flow control, an AOC-20s autosampler and a single quadrupole detector with an adjustable electron voltage of 10–195 V was utilized. A capillary column with a medium polarity RESTEK Rxi column (diameter 0.25mm, length 15 m, film thickness 0.1 μm) was used for analyses. Electron-impact ionization with electron voltage fixed at 70V and emission current set to 160 μA was used for the measurements. The temperatures of the injection port, ion source and interface were maintained at 220, 300, and 310°C, respectively. Analyses were carried out in the splitless mode with a constant linear velocity of the carrier gas (He), which was maintained at 60 cm/s. The septum purge flow was set to 3 mL/min. The samples were injected using the high pressure mode, which was applied at 200 kPa and maintained for 1 min. The detector voltage was set to 1.4 kV.
4
Amorphadiene Quantification Using GC-MS
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The samples were prepared according to the previously published
method.8 (link) Briefly, a 15% (v/v) dodecane
(Sigma-Aldrich, Zwijndrecht, The Netherlands) layer containing 56
mg/L β-caryophyllene as the internal standard was added after
induction of the bacterial cultures, thus trapping the produced amorphadiene.
After 24 h of incubation, the dodecane layers were collected and diluted
to amorphadiene concentrations ranging from 3.5 to 28 mg/L. Sample
analysis was performed on a Shimadzu GCMS-QP2010Plus system equipped
with a GC-2010 Plus gas chromatograph (GC) and an AOC-20i autoinjector.
Amorphadiene-containing extracts (2 μL) were injected splitless
into the HP-5MS (5% phenyl)-methylpolysiloxane GC column (Agilent
J&W 0.25 mm inner diameter, 0.25 μm thickness, 30 m length).
The injector temperature was set at 250 °C, and the column oven
initial temperature was started at 100 °C for 3 min, with an
increase of 15 °C/min to 130 °C and then 5 °C/min until
180 °C, followed by a temperature increase to 280 °C at
a rate of 20 °C/min and finally held for 10 min. To monitor m/z ion 189, the MS detector was set to
selected ion mode (SIM). The β-caryophyllene (Extrasynthese,
Lyon, France) standard curve was used to calculate the concentration
of amorphadiene, which was represented as the β-caryophyllene
equivalent.
method.8 (link) Briefly, a 15% (v/v) dodecane
(Sigma-Aldrich, Zwijndrecht, The Netherlands) layer containing 56
mg/L β-caryophyllene as the internal standard was added after
induction of the bacterial cultures, thus trapping the produced amorphadiene.
After 24 h of incubation, the dodecane layers were collected and diluted
to amorphadiene concentrations ranging from 3.5 to 28 mg/L. Sample
analysis was performed on a Shimadzu GCMS-QP2010Plus system equipped
with a GC-2010 Plus gas chromatograph (GC) and an AOC-20i autoinjector.
Amorphadiene-containing extracts (2 μL) were injected splitless
into the HP-5MS (5% phenyl)-methylpolysiloxane GC column (Agilent
J&W 0.25 mm inner diameter, 0.25 μm thickness, 30 m length).
The injector temperature was set at 250 °C, and the column oven
initial temperature was started at 100 °C for 3 min, with an
increase of 15 °C/min to 130 °C and then 5 °C/min until
180 °C, followed by a temperature increase to 280 °C at
a rate of 20 °C/min and finally held for 10 min. To monitor m/z ion 189, the MS detector was set to
selected ion mode (SIM). The β-caryophyllene (Extrasynthese,
Lyon, France) standard curve was used to calculate the concentration
of amorphadiene, which was represented as the β-caryophyllene
equivalent.
5
GC-MS Analysis of A. willeanum Bulb Extract
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Gas chromatography mass spectroscopy analysis was performed as previously described by Leikshmi and coworkers [15 ]. 1ml of A. willeanum H. bulb extract was analysed using a GCMS-QP2010 PlusSystem (Shimadzu, Japan). Helium was used as carrier gas in the constant flow mode at 1 ml/min. GC-MS oven initial temperature was 50 °C (for 2 min) which was gradually increased by rate of 5 °C/min up to 280 °C and maintained for 9 min. Injection port temperature was ensured as 250 °C and detector temperature was 300 °C. The ionization voltage was 70eV. 30 m long RTS volatile column was used for separation of compounds. Quadrupole Mass Detector was employed to detect compounds when they were vented from the column. Using MS data library WILEY7.LIB the spectrum was analysed and compounds were identified [15 ].
6
GC-MS Characterization of Compounds
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Measurements were carried out on a GC–MS-QP2010-Plus system (Shimadzu, Kyoto, Japan) in electron impact ionization (EI) mode. The column was DB-5MS fused silica (30 m × 2.5 mm; 0.25 μm film thickness) from Agilent, USA. Other instrumentation included an FW-80 high-speed grinder (Tianjin Taisite Instrument Co., Ltd, Tianjin, China), an AL104 electronic balance (METTLER TOLEDO Instrument Co., Ltd., Shanghai, China), a Tanon-4100 automatic digital gel image analysis system (Shanghai Tianlong Technology Co., Ltd., Shanghai, China), and an SPX-250B-Z biochemical incubator (Shanghai Bo Xun Industrial Co., Ltd., Shanghai, China)29 .
7
Comprehensive Calystegine Extract Analysis
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According to the method defined by Bourebaba et al. [27 (link)], total calystegine extract was characterized by gas chromatography–mass spectrometry analysis. First, a step of trimethylsilyl trifluoroacetamide (MSTFA) derivatization was performed preceding chromatographic analysis, and then, a GCMS-QP2010 plus system (Shimadzu, Kyoto, Japan) prepared with a DB-5 ms column (30 m × 0.25 mm I.D. × 0.25 μm df, Quadrex Corporation, Woodbridge, CT) was engaged for the characterization. The extract compounds separation was carried out in accordance with the following temperature program: initial temperature of 100 °C kept for 5 min, then increased to 300 °C at 10 °C/min, sustained during 5 min. The injection volume in split mode (split ratio 1:10) was 0.5 µl with the injector temperature at 250 °C, and the gaz carrier was He at 36.5 cm/s. interface temperature, 280 °C; ion source temperature, 250 °C; mass range, m/z 50–600; scan speed, 2500 amu/seg; and event time, 0.20 seg., were the MS detection parameters employed for the experiment. Using the GCMS solution (ver. 2.50SU3, Shimadzu) program, data collection and handling was carried out.
8
Steroid Quantification by GC-MS
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Measurements of steroid levels were done on a GCMS-QP2010 Plus system by Shimadzu (Kyoto, Japan) consisting of a gas chromatograph equipped with automatic flow control, an AOC-20s autosampler, and a single quadrupole detector with an adjustable electron voltage of 10-195 V. A capillary column with a medium polarity RESTEK Rxi column (diameter 0.25 mm, length 15 m, film thickness 0.1 μm) was used for analyses. Electronimpact ionization with electron voltage fixed at 70 V and emission current set to 160 μA was used. The temperatures of the injection port, ion source and interface were maintained at 220 °C, 300 °C, and 310 °C, respectively. Analyses were carried out in the splitless mode with a constant linear velocity of the carrier gas (He), which was maintained at 60 cm/s. The septum purge flow was set at 3 ml/min. The samples were injected using the high pressure mode (200 kPa), which was maintained for 1 min. The detector voltage was set to 1.4 kV.
9
Steroid Analysis via GCMS-QP2010 Plus
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Measurements of steroid levels were done on a GCMS-QP2010 Plus system by Shimadzu (Kyoto, Japan) consisting of a gas chromatograph equipped with automatic flow control, an AOC-20s autosampler, and a single quadrupole detector with an adjustable electron voltage of 10-195 V. A capillary column with a medium polarity RESTEK Rxi phase (diameter 0.25 mm, length 15 m, film thickness 0.1 μm) was used for analyses. Electron impact ionization with electron voltage fixed at 70 V and emission current set to 160 μA was used. The temperatures of the injection port, ion source and interface were maintained at 220 °C, 300 °C, and 310 °C, respectively. Analyses were carried out in the splitless mode with a constant linear velocity of the carrier gas (He), which was maintained at 60 cm/s. The septum purge flow was set at 3 ml/min. The samples were injected using the high pressure mode (200 kPa), which was maintained for 1 min. The detector voltage was set to 1.4 kV.
10
GC-MS Analysis of Chemical Compounds
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Measurements were carried out on a Shimadzu GC-MS-QP2010-Plus system with electron impact ionization (EI). Data were processed and elaborated with Shimadzu GC-MS Solution software (Shimadzu, v 2.70, Kyoto, Japan), equipped with a Gerstel MPS2 Multi-Purpose Sampler (Tegent, Germany). The column was ZB-5MS fused silica (30 m × 2.5 mm; 0.25 μm film thickness) from Phenomenex, USA. Other instrumentation included FW-80 high speed grinder (Tianjin Taisite Instrument Co., LTD, Tianjin, China), AL104 electronic balance (METTLER TOLEDO Instrument Co., LTD, Shanghai, China), and 20 mL headspace vial equipped with PTFE/silicone rubber pad and magnetic cap (Agilent, California, CA, USA).
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