For the calcium influx assay, all experimental results are presented as the mean±SDEV. Analyses were performed and graphs drawn in Excel (Microsoft® Office 2003, USA). For the calculation of IC50 values, the analyses were performed and graphs were drawn in Prism 3.0 (GraphPad, San Diego, CA), and results presented as the mean±SDEV. For the LDH and Trypan blue staining assays, all experimental results are presented as the mean±SEM and statistical significance of differences between the means was determined by one-way ANOVA analysis followed by Dunnett’s post hoc test to compare relevant groups with controls. Where compounds were evaluated in the absence of H2O2, comparisons were done with control of untreated cells. Where compounds were evaluated in the presence of H2O2, the comparisons were done with control of 200μMH2O2 treatment alone. The level of statistical significance was taken at p<0.05. Prism 3.0 (GraphPad, San Diego, CA) statistical software was used to perform analyses.
Prism 3
Manufactured by GraphPad
Sourced in United States, Germany
Prism 3.0 is a data analysis and graphing software developed by GraphPad. It is designed to help researchers and scientists efficiently analyze and visualize data. Prism 3.0 provides a range of tools for curve fitting, statistical analysis, and creating publication-quality graphs.
Automatically generated - may contain errors
Lab products found in correlation
Instat, by GraphPad (5 mentions)
Sigmaplot 11, by Grafiti LLC (4 mentions)
Filter holder manifold, by Merck Group (4 mentions)
Pclamp 10, by Molecular Devices (3 mentions)
Clampfit module, by Molecular Devices (3 mentions)
Stopped flow instrument, by Applied Photophysics (3 mentions)
Prism 5, by GraphPad (3 mentions)
Oxytocin ria kit, by Phoenix Pharmaceuticals (2 mentions)
Taqman assay, by Thermo Fisher Scientific (2 mentions)
Trizol rneasy kits, by Thermo Fisher Scientific (2 mentions)
Probe quantitect pcr mix, by Qiagen (2 mentions)
2.0 lightcycler amplifier, by Roche (2 mentions)
Quantitect sybr green pcr kit, by Qiagen (2 mentions)
Sas version 9, by SAS Institute (2 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (2 mentions)
Multiskan ex, by Thermo Fisher Scientific (2 mentions)
Colorimetric celltiter 96 aqueous one solution cell proliferation assay mts assay, by Promega (2 mentions)
Axiovision le, by Zeiss (2 mentions)
Whatman gf c filters, by Merck Group (2 mentions)
Ls 1701, by Beckman Coulter (2 mentions)
522 protocols using prism 3
1
Calcium Influx, LDH, and Trypan Blue Assays
2
Statistical Analysis with SigmaStat and GraphPad
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Data were analyzed using SigmaStat 3.1 software (USA). Data with parametric distribution were submitted to the one-way ANOVA test followed by the Newman-Keuls test for comparison between groups. Significance levels were adjusted to 5% (p < 0.05). The graphs will be prepared using the GraphPad Prism 3.1 software (USA). Significance levels were adjusted to 5% (p < 0.05). The graphs will be prepared using the GraphPad Prism 3.1 software (California, USA).
3
Phenobarbital Pharmacokinetic Analysis
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Descriptive parameters means, standard deviations (SD) and medians of PB PK parameters were calculated using GraphPad Prism 3.02 (GraphPad Software, Inc., La Jolla, USA). The 95 % confidence intervals (CI) for medians were calculated by method of Bonett and Price (2002) .
Linear regression models and Mann-Whitney U test were used to evaluate the impact of co-medication on PB PK parameters using GraphPad Prism 3.02 (GraphPad Software, Inc., La Jolla, USA).
Linear regression models and Mann-Whitney U test were used to evaluate the impact of co-medication on PB PK parameters using GraphPad Prism 3.02 (GraphPad Software, Inc., La Jolla, USA).
4
Cytotoxicity Evaluation of Sfb Compounds
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Data are presented as mean ± S.D. Statistical analysis was performed using the Student t test (for two experimental groups) or One-Way ANOVA followed by Newman-Keuls post hoc test (for more than two experimental groups). Significance was set at p < 0.05. Studies were performed using the GraphPad Prism 3.0 software (GraphPad Software, La Jolla, CA, USA).
In the case of cytotoxicity studies, cell viability measures (% of viable cells) were plotted as a function of the decimal logarithm of Sfb concentration. The curves were best adjusted to a sigmoid curve using the GraphPad Prism 3.0 software (GraphPad Software, La Jolla, CA, USA). The goodness of adjustments was confirmed with R2 values, which were always above 0.85.
In the case of cytotoxicity studies, cell viability measures (% of viable cells) were plotted as a function of the decimal logarithm of Sfb concentration. The curves were best adjusted to a sigmoid curve using the GraphPad Prism 3.0 software (GraphPad Software, La Jolla, CA, USA). The goodness of adjustments was confirmed with R2 values, which were always above 0.85.
5
Nonparametric Analysis of Stimulated PBMCs
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Statistical analysis was done using Prism 3 software (Graph pad version 3, LA Jolla, USA). Data was presented as mean ± SEM. Variation between the groups was calculated by non parametric Mann Whitney test. P value less than 0.05 was considered as significant. Values of stimulated PBMCs were normalised by subtracting the unstimulated values for comparison among different study subjects.
6
Tubulin Polymerization Assay Protocol
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Tubulin polymerization assays were conducted using the Tubulin Polymerization Assay Fluorescence kit (cat#BK011P, Cytoskeleton Inc.; Denver, CO) according to the manufacturer's instructions. Briefly, 100 μg of the reconstituted tubulin protein was added to each well of a pre-warmed 96-well plate and exposed to vehicle (0.1% DMSO), vincristine, docetaxel, 1 , or 2 . The absorbance excitation 360 nm and emission 450 nm was recorded every min for 1 h using a TECAN Safire II microplate reader (TECAN U.S. Inc., Research Triangle Park, NC) at 37°C. The dose-response curves were plotted using Prism 3 (Graphpad Software Inc.; San Diego, CA).
7
Statistical Analysis of Experimental Data
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Data has been expressed as mean ± SEM. To compare the significance of all pairs of columns, Newman–Keuls method using one-way analysis of variance (ANOVA) was carried out. GraphPad prism3 software was used. F values represented in the results exhibit the variability between, and within the groups and reflect the degree of freedom (df). p values represent the probability. Interestingly, these two factors are dependent on each other. Further, p-value up to 0.05 has been considered significant.
8
Behavioral and Neurochemical Analyses
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Data are expressed as mean ± SEM with n = number of identical experiments. Statistical analyses were performed using Prism-3 software (version 3.00, Graph Pad, San Diego, CA). Student’s t test (pairwise comparisons) and one-way analysis of variance (ANOVA, multiple comparisons) were used as a statistical analysis, as appropriate. Behavioral results were statistically compared with a one-way analysis of variance (ANOVA), followed by Newman-Keuls post hoc test, for comparison between experimental groups. For neurochemical analyses and immunohistochemistry experiments, results were statistically analyzed by one-way ANOVA followed by Dunnett/Tukey’s post hoc test (for unequal N). Results were considered significant at P < 0.05.
9
Statistical Analysis of Case-Control Data
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Data were first entered into Microsoft Office Excel 2007 and GraphPad Prism 3.02 program was used to analyze the data. The values were expressed as mean ± standard deviation (mean ± SD). Student’s t-test was used for comparison of means of variables between case and control subjects. The level of statistical significance was set at P<0.05 for all tests and at 95% confidence interval.
10
Statistical Analysis of Experimental Data
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Data are presented as mean ± Std. Number of animals in each group is listed in text or figure legend. Numerical data were analyzed by unpaired or paired t-test using GraphPad Prism 3 statistical software when appropriate. P<0.05 was considered as statistically significant.
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