C maf

Manufactured by Santa Cruz Biotechnology

Sourced in United States

C-Maf is a protein-coding gene that produces the c-Maf transcription factor. This protein is involved in the regulation of gene expression and plays a role in various cellular processes. The C-Maf product is a useful tool for researchers studying the functions and mechanisms of this important transcription factor.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using c maf

Western Blot Analysis of Stem Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blot analysis was performed as in(4 (link)) using antibodies to Twist1/Twist2 (ab50887; Abcam, Cambridge, MA), c-Maf (sc7866; Santa Cruz Biotechnology, Santa Cruz, CA), Bmi1 (05-1322; EMD Millipore, Billerica, MA), or anti-GAPDH (EMD Millipore).

Zheng S., Hedl M, & Abraham C. (2015). Twist1 and Twist2 contribute to cytokine downregulation following chronic NOD2 stimulation of human macrophages through the coordinated regulation of transcriptional repressors and activators. Journal of immunology (Baltimore, Md. : 1950), 195(1), 217-226.

+ Open protocol

+ Expand

ChIP Assay for HDAC11 and c-Maf in DCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

ChIP assay was performed with DCs using a reagent kit (Sigma Aldrich) following the manufacturer's instructions. Briefly, DCs were fixed with 1% formaldehyde for 15 min, lysed and sonicated to shear the chromatin DNA to 100–500 bp. Cell lysates were precleared by incubation with protein G-agarose beads for 2 h at 4°C. The supernatant was collected and incubated overnight at 4°C with 2 μg of antibodies of HDAC11 or c-Maf (Santa Cruz Biotech) or isotype IgG (a negative control). The antibody-chromatin complex was precipitated by incubation with protein G-agarose beads for 1 h at 4°C. The beads were centrifuged, washed and eluted in elution buffer. DNA was recovered from the precipitated samples by reverse crosslinking at 65°C for 4 h and digested with proteinase K for 1 h at 45°C to remove proteins, then the immunoprecipitated DNA was recovered by phenol/chloroform extraction and ethanol precipitation. The DNA or input was analyzed by PCR with the miR-19a promoter primers (Beijing Yijie Biotech; Beijing, China). The results were presented as folds of input.

Luo X.Q., Shao J.B., Xie R.D., Zeng L., Li X.X., Qiu S.Q., Geng X.R., Yang L.T., Li L.J., Liu D.B., Liu Z.G, & Yang P.C. (2017). Micro RNA-19a interferes with IL-10 expression in peripheral dendritic cells of patients with nasal polyposis. Oncotarget, 8(30), 48915-48921.

+ Open protocol

+ Expand

Western Blot and Immunoprecipitation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blot and immunoprecipitations were carried out by standard method. Antibodies used for western blotting were against β-actin (Cell Signaling, Danvers, MA, USA; 4967), NQO1 (Abcam, Cambridge, MA, USA; ab28947), p53 (Santa Cruz Biotechnology, Santa Cruz, CA, USA; sc-6243), p21 (Santa Cruz Biotechnology; sc-28777), p16 (Santa Cruz Biotechnology; sc-28777), and NRF2 (Abcam; ab62352). Antibodies used for immunoprecipitations were against p53 (Abcam; ab28), c-MAF (Santa Cruz Biotechnology; sc-7866), KEAP1 (Santa Cruz Biotechnology; sc-33569).
The densitometry data were analyzed by ImageJ software (National Institutes of Health, Bethesda, MD, USA).

Liu K., Jin B., Wu C., Yang J., Zhan X., Wang L., Shen X., Chen J., Chen H, & Mao Z. (2015). NQO1 Stabilizes p53 in Response to Oncogene-Induced Senescence. International Journal of Biological Sciences, 11(7), 762-771.

+ Open protocol

+ Expand

Interaction of RORγ, Blimp-1, and c-Maf

Check if the same lab product or an alternative is used in the 5 most similar protocols

The HEK-293T cells (2 × 10⁶ cells/well) were transected with RORγ-Flag (5 μg) and or Blimp-1-Myc (5 μg) plasmids. According to the standard procedure, immunoprecipitation and immunoblotting were performed to analyze the combination of c-Maf (1:500, sc-7866, Santa Cruz, Dallas, TX, USA), Blimp-1 (1:500, sc-47732, Santa Cruz) and RORγ (1:500 sc-81371, Santa Cruz).

Chang K.K., Liu L.B., Jin L.P., Zhang B., Mei J., Li H., Wei C.Y., Zhou W.J., Zhu X.Y., Shao J., Li D.J, & Li M.Q. (2017). IL-27 triggers IL-10 production in Th17 cells via a c-Maf/RORγt/Blimp-1 signal to promote the progression of endometriosis. Cell Death & Disease, 8(3), e2666-.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Abs for KLF13, c-Maf, and α-actinin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA), and GAPDH was purchased from Millipore (Billerica, MA). Mouse anti-V5 Ab was purchased from Invitrogen (Carlsbad, CA). Rabbit anti-FLAG Ab was purchased from Cell Signaling (Danvers, MA). For Western blot analysis, cells were lysed in cell lysis buffer (150 mM NaCl; 1 mM EDTA; 1 mM ethylene glycol-bis(β-aminoethyl ester)-N,N,N′,N′-tetraacetic acid; 1% Triton; 2.5 mM sodium pyrophosphate; 1 mM β-glycerolphosphate; 1 mM Na₂VO₄, in 20 mM Tris, pH 7.4) with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific, Rockford, IL) and incubated on ice for 30 min. After centrifugation, the supernatant proteins (containing 25–50 μg protein) were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membranes. Appropriate primary Abs and secondary Abs were used for Western blots.

Kwon S.J., Crespo-Barreto J., Zhang W., Wang T., Kim D.S., Krensky A, & Clayberger C. (2014). KLF13 Cooperates with c-Maf To Regulate IL-4 Expression in CD4+ T Cells. Journal of immunology (Baltimore, Md. : 1950), 192(12), 5703-5709.

+ Open protocol

+ Expand

Comprehensive Immunological Assays Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The fluorochrome-labeled antibodies for flow cytometry were purchased from eBioscience (San Diego, CA). The antibodies of IL-10, A20, HDAC11, Sp, IRF, AP, CREB, c/EBP, c-Maf, NF-κB, STAT3, HDAC11 shRNA kit and A20 shRNA kit were purchased from Santa Cruz Biotech (Santa Cruz, CA). LPS, ChIP kit and luciferase kit were purchased from Sigma Aldrich (St. Louis., MO). The recombinant IL-4, IL-5, IL-13 and the ELISA kit of IL-4 were purchased from R&D Systems (Minneapolis, MN). The OVA-specific IgE ELISA kit was purchased from the Biomart (Beijing, China). The reagent kits for magnetic cell sorting were purchased from Miltenyi Biotech (San Diego, CA). Reagents for RT-qPCR and Western blotting were purchased from Invitrogen (Carlsbad, CA).

Li M.Y., Zhu M., Linghu E.Q., Feng F., Zhu B., Wu C, & Guo M.Z. (2016). Interleukin-13 suppresses interleukin-10 via inhibiting A20 in peripheral B cells of patients with food allergy. Oncotarget, 7(48), 79914-79924.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!