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2 protocols using paclitaxel t7191

1

Sensitizing Cervical Cancer Cells

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The human cervical cancer cell lines HeLa (HPV 18-positive) and CaSki (HPV16-positive) were obtained from the ATCC (Manassas, VA). Cells were routinely tested for mycoplasma and characterized by short tandem report profiling (AmpFISTR identifier kit, Applied Biosystems, Foster, CA, cat. 4322288). HeLa and CaSki cells were transfected with siRNAs using DharmaFECT transfection reagent (Thermo Scientific, USA) according to the manufacturer's protocol. The HPV 16 and 18 siRNA sequences are listed in Table S1. Cisplatin (P4394) and paclitaxel (T7191) were obtained from Sigma-Aldrich, USA. For combination therapy, cells were exposed to E6/E7 siRNA along with chemotherapeutic agents.
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2

Cinnamaldehyde-Induced Apoptosis Pathway

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Cinnamaldehyde (CA; W228613), cisplatin (1134357), paclitaxel (T7191), (Z-VAD-FMK; caspase inhibitor; V116), 3-methyladenine (3-MA; autophagy inhibitor; M9281), chloroquine (CQ; autophagy inhibitor; C6628), compound C (AMPK inhibitor; P5499), SBI-0206965 (ULK1 inhibitor; SML1540), BIX-01294 (G9a inhibitor; B9311), and thapsigargin (TG; ER stress inducer; T9033) were purchased from Sigma-Aldrich (St. Louis, MO USA).
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