Agilent 1100 series binary hplc pump

All capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) experiments were performed using Agilent 7100 CE capillary electrophoresis (Agilent Technologies, Waldbronn, Germany), the Agilent 6230 LC/MSD TOF system (Agilent Technologies, Palo Alto, CA, USA), an Agilent1100 series binary HPLC pump, and the G1603A Agilent CE-MS adapter- and G1607A Agilent CE-ESI–MS sprayer kit. For anionic metabolite analysis, the original Agilent stainless electrospray ionization (ESI) needle was replaced with an Agilent G7100-60,041 platinum ESI needle (Soga et al., 2009 (link)). System control and data acquisition were performed using the Agilent MassHunter Workstation, and data analysis was performed using Keio MasterHands software.

Mass spectrometer analysis was performed using the linear ion trap-Fourier transform ion cyclotron resonance mass spectrometer (LTQ-FTICR MS; Thermo Fisher) equipped with a nano-electrospray ion source (New Objective) and a nano-HPLC system. Nano-HPLC separation used a reverse nano-column (75 μm I.D. × 200 mm) packaged with Magic C18AQ resin (particle size 5 μm, pore size 200 A°; Michrom Bioresources) and an Agilent 1100 series binary HPLC pump (Agilent Technologies). The analytic program was set at a linear gradient from 10% to 50% ACN with a 60 min running cycle. The survey scan of MS analysis (m/z 320–2,000) was performed in LTQ-FTICR MS with a mass resolution of 100,000 at m/z 400. Top ten most abundant multiply charged ions were sequentially isolated for MS/MS by LTQ. The resulting data were applied to the MaxQuant software [23 (link)] for protein identification. Accurate label-free quantification was performed using the MaxLFQ program by normalization and maximal peptide ratio extraction methods [24 (link)]. The significance threshold for the identification was set to P < .01.

Plasma, urinary, and fecal levels of fecal purine metabolites including allantoin were measured using capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) as described previously [15 (link)]. All CE-TOFMS experiments were performed using an Agilent CE capillary electrophoresis system (Agilent Technologies, Santa Clara, CA, USA), an Agilent G3250AA LC/MSD TOF system (Agilent Technologies), an Agilent 1100 series binary HPLC pump, a, G1603A Agilent CE-MS adapter, and a G1607A Agilent CE-ESI-MS sprayer kit.

All capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) experiments were performed using Agilent 7100 CE capillary electrophoresis (Agilent Technologies), the Agilent 6230 LC/MSD TOF system (Agilent Technologies), an Agilent1100 series binary HPLC pump, and the G1603A Agilent CE-MS adapter- and G1607A Agilent CE-ESI-MS sprayer kit. For anionic metabolite analysis, the original Agilent stainless ESI needle was replaced with the Agilent G7100-60041 platinum ESI needle.⁶⁸ (link) System control and data acquisition were performed by Agilent MassHunter Workstation, and data analysis was done by Keio MasterHands software.