Tj905

Human normal glial cell HEB and glioma cell lines (U87, TJ861, TJ905, U251, H4, and A172) were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). The cells were cultured with RPMI1640 containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Invitrogen, CA, USA)) at 37℃ and 5% CO₂. RPMI1640 and FBS were provided by Thermo Fisher Scientific (MA, USA). During the logarithmic growth phase, cells were treated with 0.25% trypsin (Thermo Fisher, HyClone, Utah, USA) for trypsinization and passage. It was found that CircPIP5K1A was the lowest expressed in U87 cells and the highest expressed in A172. Therefore, U87 and A172 cells were chosen as the research object in subsequent studies.

Human HEB and GBM cells (LN229, U251, U87, T98G, GOS3, and TJ905) were purchased from American Type Culture Collection (ATCC). These cells were authenticated using an STR assay (Beijing Genomics institution). HEB, LN229, U251, U87, GOS3, and TJ905 cells were cultured in DMEM containing 10% FBS (Clark Bioscience, Australia); T98G cells were cultured in MEM containing NEAA and 10% FBS (Clark Bioscience, Australia) and grown in a humidified incubator at 37 °C in an atmosphere of 5% CO₂.