Advia 120e

Each blood sample was transferred to CBC bottles (EDTA 3 K; BD Biosciences, San Jose, CA, USA) and assayed on a hematology analyzer (Advia 120E; Siemens Healthcare, Malvern, PA USA). Hematological measurements included eosinophil (EOS), mean corpuscular hemoglobin concentration (MCHC), hematocrit (HCT), neutrophil (NEU), hemoglobin (HGB), mean corpuscular volume (MCV), large unstained cell (LUC), lymphocyte (LYM), red blood cell (RBC), white blood cell (WBC), platelet (PLT), mean corpuscular hemoglobin (MCH), monocyte (MONO), mean platelet volume (MPV), basophil (BASO), red cell distribution width (RDW), and hemoglobin distribution width (HDW).

The rats were fasted overnight before necropsy. Blood was collected from the abdominal aorta under anesthesia with isoflurane. Three milliliters of blood was collected in a CBC bottle (EDTA 3 K; BD Biosciences, San Jose, CA, USA) and analyzed using an autohematoanalyzer (Advia120E; Siemens). The remaining blood was transferred into Vacutainer^® tubes (sodium citrate 3.2%, BD Biosciences) and centrifuged at 3,000 rpm for 10 minutes. The plasma was separated and clotting time was determined using a coagulometer (ACL 7000; Instrumentation Laboratory, Bedford, MA, USA). The hematological parameters studied were as follows: total leukocyte and differential leukocyte (neutrophil, lymphocyte, monocyte, eosinophil, and basophil) count, total erythrocyte count, hemoglobin concentration, hematocrit, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration, reticulocytes, platelets, prothrombin time, and activated partial thromboplastin time.

The animals were fasted overnight before necropsy. Blood was collected from the abdominal aorta under anesthesia with isoflurane. We collected 3 mL of blood in a CBC (counting blood cells) bottle (ethylenediaminetetraacetic acid 3 K; Becton, Dickinson and Company, Franklin Lakes, NJ, USA) and analyzed it using an autohematoanalyzer (ADVIA120E; Siemens AG). Other blood was transferred into vacutainer tubes (sodium citrate 3.2%; Becton, Dickinson and Company) and centrifuged at 3,000 rpm for 10 minutes. The plasma was separated and used to determine clotting time by using a coagulometer (ACL 7000; Instrumentation Laboratory, Bedford, MA, USA). Hematological parameters were as follows: total leukocyte and differential leukocyte (neutrophil, lymphocyte, monocyte, eosinophil, and basophil) counts, total erythrocyte count, hemoglobin concentration, hematocrit (Ht) level, mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC), reticulocyte, platelet, prothrombin time, and activated partial thromboplastin time.